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m5zn_a4ac3a22336dedd
m5zn_a4ac3a22336dedd

... the flow (or transcription) of genetic information from DNA to mRNA. Transcription factors perform this function alone or with other proteins in a complex, by promoting (as an activator), or blocking (as a repressor) the recruitment of RNA polymerase (the enzyme that performs the transcription of ge ...
CLN Carbohydrat es part3
CLN Carbohydrat es part3

... include: Lactose (glucose and galactose) and maltose (malt product) and are both ...
Document
Document

... • Fermentative result: Acid production on both (open and covered) tubes. The acid produced changes the pH indicator, bromthymol blue, from green to yellow. e.g. Escherichia coli • Oxidative result: Acid production in the open tube (aerobic) and not the oil-covered tube (anaerobic) indicates an oxid ...
experiment six
experiment six

... To collect the vein blood and separate the serum before or after injection, respectively To measure the concentration of blood glucose in serum, and compare the change of blood glucose level before or after injection of hormone To confirm the effect of adrenalin and insulin on blood glucose level ...
glycogen disappears
glycogen disappears

... • Glycogen represents the principal storage form of carbohydrate in the mammalian body, mainly in the liver and muscle. • In the liver, its major function is to provide glucose for extrahepatic tissues. In muscle, it serves mainly as a ready source of metabolic fuel for use in muscle. • Glycogen is ...
presentation source
presentation source

... Adrenergic receptors, second messenger and effects of catecholamines ...
Glucose (C6H12O6), also known as D
Glucose (C6H12O6), also known as D

... People with this deadly disease must perform regular checks on their glucose level in their blood. The most inexpensive and widely used way of monitoring glucose level is to poke their fingers to draw the blood needed for the test. A 2011 report projected that the global blood glucose test strips an ...
From DNA sequence to application: possibilities and
From DNA sequence to application: possibilities and

... sequences.Comparison of the complete nucleotide sequencesof several LAB bacteriophages has revealed that their chromosomeshave a fixed, modular structure, each module having a set of genesinvolved in a specific phase of the bacteriophage life cycle. LAB bacteriophage genesand DNA sequenceshave been ...
High carbohydrate diet : which reduces gluconeogenesis by
High carbohydrate diet : which reduces gluconeogenesis by

... Some tissues, such as the brain, red blood cells, kidney medulla, lens and cornea of the eye, tests and exercising muscle, require a continuous supply of glucose as a metabolic fuel. Liver glycogen, can meet these needs for only ten to eighteen hours in the absence of dietary intake of carbohydrates ...
pdf
pdf

... classic systems in which these issues have been explored are antitermination in bacteriophage λ and in attenuation of transcription in bacterial biosynthetic operons, in particular the trp operon in E. coli. Although some of the mechanistic details may be peculiar to bacteria, especially the need fo ...
Control of Gene Expression
Control of Gene Expression

... how often transcription is initiated. Regulatory DNA sequences do not work by themselves. To have any effect, these sequences must be recognized by proteins called transcription regulators,which bind to the DNA. It is the combination of a DNA sequence and its associated protein molecules that acts a ...
How do bacteria respond to their environment?
How do bacteria respond to their environment?

... to nitrogen deprivation Stringent response • Make less ribosomal protein • Make less ribosomal RNA • Make less transfer RNA ...
metabolism and function of carbohydrates
metabolism and function of carbohydrates

... gastro enteric path they work and which products they form? 7. Which enzymes hydrolyze polysaccharides? What is their specificity? (what kind of bond they hydrolyze?) In which departments of a gastro enteric path they work and which products they form? 8. Name carbohydrate which is important dietary ...
1. A Draw the structure of glucose using either a ring or straight
1. A Draw the structure of glucose using either a ring or straight

... C. Ferredoxin (Fd) is an iron containing protein present in the photosynthetic apparatus; during photosynthesis the iron undergoes a 1-electron redox reaction with an Eo' = -0.42v. Similarly cytochrome b5 is an iron-protein present in the microsomes with an Eo' = 0.0 v. For the reaction at pH = 7 ...
CARBOHYDRATES 2016
CARBOHYDRATES 2016

... Sucrose, common table sugar, has a glycosidic bond linking the anomeric hydroxyls of glucose & fructose. Because the configuration at the anomeric C of glucose is α (O points down from ring), the linkage is α(1→2). The full name of sucrose is α-D-glucopyranosyl-(1→2)-β-Dfructopyranose.) Lactose, mil ...
SUMMARY Evidence 1s summarized showing that thymine methyls
SUMMARY Evidence 1s summarized showing that thymine methyls

... glucosyl-hydroxymethyl group at C5 and the virus encodes most enzymes necessary to replicate and express such DNA (29). Furthermore, only one or a few thymines per gene are likely to be the important ones to replace with ...
Network Regulation of the Escherichia coli Maltose System
Network Regulation of the Escherichia coli Maltose System

... regulator Mlc is a repressor for malT transcription. The repressor activity of Mlc is controlled by the transport status of the glucose-specific enzyme EIICB of the PTS that causes sequestration (and inactivation as a repressor) of Mlc when glucose is transported. The second level of MalT regulation ...
What limits the liver`s capacity to convert amino acids to glucose?
What limits the liver`s capacity to convert amino acids to glucose?

... liver is most likely the result of degradation of hepatic proteins. Amino acids from these are rapidly used as substrate for gluconeogenesis. Alanine from skeletal muscles is also a major contributor to hepatic glucose production. However, degradation of muscle proteins begins somewhat later than he ...
finalcarbohydrat met..
finalcarbohydrat met..

... B. It is activated by chloride ions (cl-). C. It acts on cooked starch and glycogen breaking α 1-4 bonds, converting them into maltose [a disaccharide containing two glucose molecules attached by α 1-4 linkage]. This bond is not attacked by -amylase. Because both starch and glycogen also contain 1-6 ...
Exam 2 Answer Key Spring 1996 Mcbio 316 - page 1
Exam 2 Answer Key Spring 1996 Mcbio 316 - page 1

... As expected, ØPK2 would be repressed by a ØPK2 lysogen c. Suggest a simple explanation for the behavior of ØPK1 on each of these strains. Either the repressor produced by the ØPK2 lysogen can also repress any superinfecting ØPK1 (that is, the two phage are homoimmune) or the ØPK2 lysogen produces a ...
PowerPoint Slides - Evo-Ed
PowerPoint Slides - Evo-Ed

... • In oxic conditions, the cit operon promoter is still inhibited by a repressor protein. • The genes in the cit operon, including the original copy of citT are not transcribed. • However, the promoters downstream of the cit operon are not (and never were) repressed by oxygen. ...
Table 3S
Table 3S

... in a reverse pathway of synthesis of Cys from Met (Rodionov et al., 2004). The yxeK operon, yhcL, and ytlI are known to be strongly up-regulated during growth on methionine as sole sulfur source (relative to sulfate-grown cells; (Auger et al., 2002) . YhcL is a symporter that functions in the uptake ...
Transcription – Gene regulation
Transcription – Gene regulation

... Computational identification of transcription factor binding sites is difficult because they consist of short, degenerate sequences that occur frequently by chance. The problem is not easy to define (therefore: it is „complex“) because - the motif is of unknown size - the motif might not be well con ...
Document
Document

... amino groups, sulfated hydroxyl groups,etc. Glycosaminoglycans tend to be negatively charged because of the presence of acidic groups. It is important component of connective tissues. Some examples of glycosaminoglycan in nature include heparin as an anticoagulant , hyaluronic acid as a component of ...
Genetic Markers of E. coli
Genetic Markers of E. coli

... Overproduction of the lac repressor protein Leads to high levels of the lac repressor protein, inhibiting transcription from the lac promoter. lacY Galactoside permease mutation Blocks lactose utilization. lacZ∆M15 Partial deletion of β-d-galactosidase gene Allows complementation of β-galactosidase ...
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Lac operon



lac operon (lactose operon) is an operon required for the transport and metabolism of lactose in Escherichia coli and many other enteric bacteria. Although glucose is the preferred carbon source for most bacteria, the lac operon allows for the effective digestion of lactose when glucose is not available. Gene regulation of the lac operon was the first genetic regulatory mechanism to be understood clearly, so it has become a foremost example of prokaryotic gene regulation. It is often discussed in introductory molecular and cellular biology classes at universities for this reason.Bacterial operons are polycistronic transcripts that are able to produce multiple proteins from one mRNA transcript. In this case, when lactose is required as a sugar source for the bacterium, the three genes of the lac operon can be expressed and their subsequent proteins translated: lacZ, lacY, and lacA. The gene product of lacZ is β-galactosidase which cleaves lactose, a disaccharide, into glucose and galactose. LacY encodes lactose permease, a protein which becomes embedded in the cytoplasmic membrane to enable transport of lactose into the cell. Finally, lacA encodes galactoside O-acetyltransferase. Layout of the lac operon.It would be wasteful to produce the enzymes when there is no lactose available or if there is a more preferable energy source available, such as glucose. The lac operon uses a two-part control mechanism to ensure that the cell expends energy producing the enzymes encoded by the lac operon only when necessary. In the absence of lactose, the lac repressor halts production of the enzymes encoded by the lac operon. In the presence of glucose, the catabolite activator protein (CAP), required for production of the enzymes, remains inactive, and EIIAGlc shuts down lactose permease to prevent transport of lactose into the cell. This dual control mechanism causes the sequential utilization of glucose and lactose in two distinct growth phases, known as diauxie.
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