Lactose Uptake Driven by Galactose Efflux in Streptococcus
... proton symport and a lactose permease (18). That galactosefermenting mutants of S. thermophilus could be derived from wild-type Gal- strains (21), and that the former transported galactose at a significantly greater rate than the Galparental strains, would support the presence of distinct galactose ...
... proton symport and a lactose permease (18). That galactosefermenting mutants of S. thermophilus could be derived from wild-type Gal- strains (21), and that the former transported galactose at a significantly greater rate than the Galparental strains, would support the presence of distinct galactose ...
Cloning and nucleotide sequence of a gene upstream of the eaeA
... alvei, and E. coli strain RDEC-1 that causes diarrhea in rabbits, and is thought to be a hot spot for insertion of virulence factor genes in the E. co/i chromosome. Lai and Donnenberg [ 161 reported that genes located between eueA and eaeB as well as downstream of eaeB are required for attaching and ...
... alvei, and E. coli strain RDEC-1 that causes diarrhea in rabbits, and is thought to be a hot spot for insertion of virulence factor genes in the E. co/i chromosome. Lai and Donnenberg [ 161 reported that genes located between eueA and eaeB as well as downstream of eaeB are required for attaching and ...
Carbohydrate metabolism File
... protein phosphatase-1, the enzyme is in activated to phosphorylase b in a reaction that involves hydrolytic removal of the phosphate from the serine residue. Reactivation requires rephosphorylation with ATP and a specific enzyme, phosphorylase kinase ...
... protein phosphatase-1, the enzyme is in activated to phosphorylase b in a reaction that involves hydrolytic removal of the phosphate from the serine residue. Reactivation requires rephosphorylation with ATP and a specific enzyme, phosphorylase kinase ...
LIVER GENE EXPRESSION DURING THE TRANSITION DURING THE DRY PERIOD
... effects of ad libitum or restricted intake of moderate-energy diets during the entire dry period on pre-partum metabolism and post-partum metabolism and performance. A corn silage-based diet (26% of diet dry matter) providing 0.72 Mcal/lb during the far-off dry period (first 5 wk of an 8wk dry perio ...
... effects of ad libitum or restricted intake of moderate-energy diets during the entire dry period on pre-partum metabolism and post-partum metabolism and performance. A corn silage-based diet (26% of diet dry matter) providing 0.72 Mcal/lb during the far-off dry period (first 5 wk of an 8wk dry perio ...
Chapter 15 Cori and Alanine Cycles: Cori Cycle: Occurs between
... Glucose from Fatty Acids: →Usually, fatty acids have an even number of carbon atoms FAeven →→ Acetyl-CoA X which is not converted to glucose FAodd →→ Acetyl-CoA + a single propionyl-CoA→ proprionate→oxaloacetate TAG → glycerol backbone → DHAP →glucose ...
... Glucose from Fatty Acids: →Usually, fatty acids have an even number of carbon atoms FAeven →→ Acetyl-CoA X which is not converted to glucose FAodd →→ Acetyl-CoA + a single propionyl-CoA→ proprionate→oxaloacetate TAG → glycerol backbone → DHAP →glucose ...
... iii) Why are transport proteins (e.g. K+ channel) considered (at least by me) to be enzymes, even though they do not change the chemical structure of the substrate? (1 pt) i) Good substrates have many interactions with the active site, leading to small off-rates. Therefore the substrate is bound lon ...
งานนำเสนอ PowerPoint
... permit its substrate and product to equilibrate • most of other reactions in a pathway function close to equilibrium • committed step = rate-limiting step ...
... permit its substrate and product to equilibrate • most of other reactions in a pathway function close to equilibrium • committed step = rate-limiting step ...
GENETICS TEST IV - Daytona State College
... • A nucleotide sequence organized as triplets that encodes the amino acid sequence of a polypeptide, including an initiation codon and a termination codon. • In some viruses, overlapping genes have been identified in which initiation at different AUG positions out of frame with one another leads to ...
... • A nucleotide sequence organized as triplets that encodes the amino acid sequence of a polypeptide, including an initiation codon and a termination codon. • In some viruses, overlapping genes have been identified in which initiation at different AUG positions out of frame with one another leads to ...
Alkaptonuria and Aspergillus nidulans
... The clinical features of the disease ten to fall into two categories, acute and chronic. In the so-called acute form of the disease, abnormalities appear in the first month of life. Babies may show poor weight gain, an enlarged liver and spleen, a distended abdomen, swelling of the legs, and an incr ...
... The clinical features of the disease ten to fall into two categories, acute and chronic. In the so-called acute form of the disease, abnormalities appear in the first month of life. Babies may show poor weight gain, an enlarged liver and spleen, a distended abdomen, swelling of the legs, and an incr ...
Ketamalt® 50
... – sugars, starches, amino acids and propylene glycol. In addition, Ketamalt® contains DIASTASE, a natural starch converting enzyme that converts starches present in the rumen to simple sugars. Propylene glycol is only one source of oral glucose precursor used to reverse ketosis in ruminants. Althoug ...
... – sugars, starches, amino acids and propylene glycol. In addition, Ketamalt® contains DIASTASE, a natural starch converting enzyme that converts starches present in the rumen to simple sugars. Propylene glycol is only one source of oral glucose precursor used to reverse ketosis in ruminants. Althoug ...
Additional file 5: Method SI Constructions of other knock
... introduced into strain 5008 by intergeneric conjugation, and single-crossover exconjugants were screened by thiostrepton resistance (TsrR). Subsequently, marker-free deletion mutant JG34 was selected from the initial TsrR exconjugants after several rounds of nonselective growth, and confirmed by PCR ...
... introduced into strain 5008 by intergeneric conjugation, and single-crossover exconjugants were screened by thiostrepton resistance (TsrR). Subsequently, marker-free deletion mutant JG34 was selected from the initial TsrR exconjugants after several rounds of nonselective growth, and confirmed by PCR ...
CHEM 331 Problem Set #6
... 6. The enzyme glucose oxidase isolated from the mold Penicillium notatum catalyzes the oxidation of β-D-glucose to D-glucono-δ-lactone. This enzyme is highly specific for the β anomer of glucose and does not affect the α anomer. In spite of this specificity, the reaction catalyzed by glucose oxidas ...
... 6. The enzyme glucose oxidase isolated from the mold Penicillium notatum catalyzes the oxidation of β-D-glucose to D-glucono-δ-lactone. This enzyme is highly specific for the β anomer of glucose and does not affect the α anomer. In spite of this specificity, the reaction catalyzed by glucose oxidas ...
A glucose-responsive transcription factor that regulates
... tribution, specificity, and carbohydrate responsiveness of ChREBP’s DNA-binding activity are consistent with those of the putative glucose-responsive transcription factor. Furthermore, ChREBP contains several consensus phosphorylation sites that may regulate the activity of this protein. These data ...
... tribution, specificity, and carbohydrate responsiveness of ChREBP’s DNA-binding activity are consistent with those of the putative glucose-responsive transcription factor. Furthermore, ChREBP contains several consensus phosphorylation sites that may regulate the activity of this protein. These data ...
Candida albicans MBP1
... separating and growing them on selective media. A mbp1/swi4 double mutant was isolated using this technique in order to assess the activity of C. albicans MBP1. B) Gene disruptions of MBP1 via Geneticin (G418) resistance cassette (KNMX4) in YJJ1000 strain and Swi4 disruption using a URA3 cassette in ...
... separating and growing them on selective media. A mbp1/swi4 double mutant was isolated using this technique in order to assess the activity of C. albicans MBP1. B) Gene disruptions of MBP1 via Geneticin (G418) resistance cassette (KNMX4) in YJJ1000 strain and Swi4 disruption using a URA3 cassette in ...
The Enterobacteriaceae
... NAD is reduced to NADH2 by accepting electrons during glycolytic conversion of glucose to pyruvate NADH2 in turn reduces pyruvate with oxidation of NADH2 to NAD which supports continued anaerobic glycolysis, and generation from pyruvate of alcohols, carboxylic acids, and CO2 gas End products of gluc ...
... NAD is reduced to NADH2 by accepting electrons during glycolytic conversion of glucose to pyruvate NADH2 in turn reduces pyruvate with oxidation of NADH2 to NAD which supports continued anaerobic glycolysis, and generation from pyruvate of alcohols, carboxylic acids, and CO2 gas End products of gluc ...
intolerance to lactose and other dietary sugars
... (Madzarovova-Nohejlova, 1973). Given the unusual distribution of the disaccharide, deficiency has not been of any nutritional consequence until recently, but it may become a problem because of its introduction as a sweetener in foods. Sodium-Dependent Glucose Transporter (SGLT1). SGLT1 is encoded by ...
... (Madzarovova-Nohejlova, 1973). Given the unusual distribution of the disaccharide, deficiency has not been of any nutritional consequence until recently, but it may become a problem because of its introduction as a sweetener in foods. Sodium-Dependent Glucose Transporter (SGLT1). SGLT1 is encoded by ...
NEISSERIA
... Specimen: urethral,cervical smears &swabs (transport medium). Gram film: intracellular Gram -ve diplococci Culture: selective media Oxidase +ve acid production from glucose Latex agglutination ...
... Specimen: urethral,cervical smears &swabs (transport medium). Gram film: intracellular Gram -ve diplococci Culture: selective media Oxidase +ve acid production from glucose Latex agglutination ...
NEISSERIA
... Specimen: urethral,cervical smears &swabs (transport medium). Gram film: intracellular Gram -ve diplococci Culture: selective media Oxidase +ve acid production from glucose Latex agglutination ...
... Specimen: urethral,cervical smears &swabs (transport medium). Gram film: intracellular Gram -ve diplococci Culture: selective media Oxidase +ve acid production from glucose Latex agglutination ...
RBTopic3_6 Enzymes - wfs
... this, the reactions may occur faster. Enzymes are organic catalysts. They are proteins. 2. The amino acids that make up these enzymes allow a tertiary and/or quaternary structure. Because each enzyme has a specific amino acid sequence, enzymes have a specific three-dimensional shape. 3. The molecule ...
... this, the reactions may occur faster. Enzymes are organic catalysts. They are proteins. 2. The amino acids that make up these enzymes allow a tertiary and/or quaternary structure. Because each enzyme has a specific amino acid sequence, enzymes have a specific three-dimensional shape. 3. The molecule ...
First Homework Assignment
... reducing sugars and the beta linkages would be hydrolyzed by a beta-galactosidase. Similarly, connecting glucose as a glucopyranoside to galactose also gives 8 possible types of linkage. These are all reducing sugars and the alpha type of linkage would be cut by an alpha glucosidase. Finally, the tw ...
... reducing sugars and the beta linkages would be hydrolyzed by a beta-galactosidase. Similarly, connecting glucose as a glucopyranoside to galactose also gives 8 possible types of linkage. These are all reducing sugars and the alpha type of linkage would be cut by an alpha glucosidase. Finally, the tw ...
Endocrinology – glucose homeostasis
... • Acts primarily on the liver, where it stimulates glycogenolysis and gluconeogenesis and thus increases hepatic glucose output. Glucagon also stimulates ketogenesis, providing an alternative fuel for those tissues that can use it and sparing glucose for those that cannot do without. • Also causes l ...
... • Acts primarily on the liver, where it stimulates glycogenolysis and gluconeogenesis and thus increases hepatic glucose output. Glucagon also stimulates ketogenesis, providing an alternative fuel for those tissues that can use it and sparing glucose for those that cannot do without. • Also causes l ...
reprint - Oleg Igoshin
... Similarly, cotranscription of multiple (redundant) gene regulators from the same operon results in increased CV in the regulated gene as compared to the uncoupled regulator configuration (Figures 2D and S1E, N). Here, we assumed that the gene regulatory logic was an OR gate (i.e., each regulator by ...
... Similarly, cotranscription of multiple (redundant) gene regulators from the same operon results in increased CV in the regulated gene as compared to the uncoupled regulator configuration (Figures 2D and S1E, N). Here, we assumed that the gene regulatory logic was an OR gate (i.e., each regulator by ...
Glucose Metabolism
... A. Glucose in the bloodstream comes from the digestion and/or from glycogen stored in the liver and muscle. B. When glucose in the bloodstream enters the cytosol (internal fluid) of our cells, it is immediately converted to glucose – 6 – phosphate. 1. This is an exergonic process and not reversible. ...
... A. Glucose in the bloodstream comes from the digestion and/or from glycogen stored in the liver and muscle. B. When glucose in the bloodstream enters the cytosol (internal fluid) of our cells, it is immediately converted to glucose – 6 – phosphate. 1. This is an exergonic process and not reversible. ...
Lac operon
lac operon (lactose operon) is an operon required for the transport and metabolism of lactose in Escherichia coli and many other enteric bacteria. Although glucose is the preferred carbon source for most bacteria, the lac operon allows for the effective digestion of lactose when glucose is not available. Gene regulation of the lac operon was the first genetic regulatory mechanism to be understood clearly, so it has become a foremost example of prokaryotic gene regulation. It is often discussed in introductory molecular and cellular biology classes at universities for this reason.Bacterial operons are polycistronic transcripts that are able to produce multiple proteins from one mRNA transcript. In this case, when lactose is required as a sugar source for the bacterium, the three genes of the lac operon can be expressed and their subsequent proteins translated: lacZ, lacY, and lacA. The gene product of lacZ is β-galactosidase which cleaves lactose, a disaccharide, into glucose and galactose. LacY encodes lactose permease, a protein which becomes embedded in the cytoplasmic membrane to enable transport of lactose into the cell. Finally, lacA encodes galactoside O-acetyltransferase. Layout of the lac operon.It would be wasteful to produce the enzymes when there is no lactose available or if there is a more preferable energy source available, such as glucose. The lac operon uses a two-part control mechanism to ensure that the cell expends energy producing the enzymes encoded by the lac operon only when necessary. In the absence of lactose, the lac repressor halts production of the enzymes encoded by the lac operon. In the presence of glucose, the catabolite activator protein (CAP), required for production of the enzymes, remains inactive, and EIIAGlc shuts down lactose permease to prevent transport of lactose into the cell. This dual control mechanism causes the sequential utilization of glucose and lactose in two distinct growth phases, known as diauxie.