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Acute nutritional ketosis: implications for exercise performance and metabolism Open Access
Acute nutritional ketosis: implications for exercise performance and metabolism Open Access

... with growing evidence to support a performanceenhancing effect during an exercise longer than 1 h [43,44] (for reviews, see [45-47]). Numerous studies have investigated dietary and pharmacological strategies to increase the exogenous provision of carbon units to the TCA cycle during exercise. Confli ...
Vitamin B2 -- Riboflavin
Vitamin B2 -- Riboflavin

... associated with an increased risk of cervical dysplasia. The effect of certain drugs on nutrient metabolism is discussed. Antituberculotic drugs such as INH and cycloserine interfere with vitamin B6 metabolism and may produce a secondary niacin deficiency. Oral contraceptives interfere with the meta ...
Selected reaction monitoring applied to proteomics
Selected reaction monitoring applied to proteomics

... thereof are enzymatically digested to generate peptides, which in turn are analyzed by liquid chromatography coupled to mass spectrometry. This shotgun approach is a powerful tool to identify proteins in complex biological samples as exemplified by a wealth of publications.[1,2] However, it is not o ...
Flux Balance Analysis of Photoautotrophic
Flux Balance Analysis of Photoautotrophic

... System-wide metabolic flux characterization is recognized as an important part of metabolic engineering not only in microbial systems but also in plants (2). One approach toward a quantitative understanding of the interactions between light and central metabolism is through kinetic modeling (3, 4 an ...
msb145697-sup-0001-Supp_Info
msb145697-sup-0001-Supp_Info

... Figure S5. Estimation of coverage of total protein mass by mass spectrometry. To estimate the fraction of total protein mass covered by mass spectrometry, we rely on two pieces of information: 1) the highly non-uniform distribution of individual protein mass as given by the method of spectral count ...
in vitro biotransformation of sildenafil (viagra) in the male rat: the role
in vitro biotransformation of sildenafil (viagra) in the male rat: the role

... incubated in triplicate. At 37°C, sildenafil (36 ␮M) was incubated for 30 min with 10 ␮g of microsomes and either 50 mM potassium phosphate buffer, 100 ␮g of control serum (goat or rabbit), or 100 ␮g of each polyclonal antirat P450 antibody (Gentest, Woburn, MA). A NADPH-regenerating cofactor system ...
Metabolic decision making by protein-metabolite - ETH E
Metabolic decision making by protein-metabolite - ETH E

... covalent  posttranslational  modifications  possible.  In  contrast,  a  lack  of  analogous  methods  for  the  investigation of allosteric interactions remains a major obstacle in understanding their regulatory role.  For both regulatory layers, the current key challenge is to identify those postt ...
INSIDER`S GUIDE Interpretation and treatment: Organic acid
INSIDER`S GUIDE Interpretation and treatment: Organic acid

... The citric acid cycle, also known as the tricarboxylic acid cycle (TCA cycle) or the Krebs cycle occurs in the matrix of the mitochondrion. The citric acid cycle is the all important metabolic pathway which uses a series of enzymecatalysed chemical reactions involved in the conversion of carbohydra ...
Functional lipidomics of oxidized products from polyunsaturated fatty
Functional lipidomics of oxidized products from polyunsaturated fatty

... The former enzymes lead to the unstable cyclic prostaglandin endoperoxides, PGH, from dihomogamma-linolenic, arachidonic and eicosapentaenoic acids, namely PGH1, H2 and H3, because the enzyme activity is associated with hydroperoxidase in the same protein (PGH synthase) (Smith 2008). A series of PGD ...
L-LACTATE Assay Kit KC-04-001 (Fluorometric) 100 tests (96 well
L-LACTATE Assay Kit KC-04-001 (Fluorometric) 100 tests (96 well

... *These reagents are stable during 10 days at 4ºC and is shipped in these conditions. Once received keep them at -20ºC. ...
Exploring the Biosynthetic Potential of Cystobacter fuscus
Exploring the Biosynthetic Potential of Cystobacter fuscus

... im letzten Jahr meiner Arbeit. Bei Katrin Jungmann und Hilda Sucipto für das Beantworten meiner vielen Fragen rund um die Molekularbiologie sowie bei Dr. Thomas Hoffmann, der mir von Anfang an immer mit Rat und Tat zur Seite stand. Außerdem bei ...
Identifying Candidates for VNS Therapy®
Identifying Candidates for VNS Therapy®

... cMorrell MJ. In: Wyllie E, ed. The Treatment of Epilepsy: Principles and Practice. 2nd ed. Baltimore, Md: Williams ...
Dynamic changes of yak (Bos grunniens) gut microbiota during
Dynamic changes of yak (Bos grunniens) gut microbiota during

... alignment length, and finally we identified a total of 6754 KEGG orthologous groups (KOs). Classifications of ...
CONTRIBUTIONS OF ANAEROBIC METABOLISM TO pH
CONTRIBUTIONS OF ANAEROBIC METABOLISM TO pH

... NAD + ratio, which are parameters that may be thought of as being general indicators of the metabolic state of a tissue (Erecinska & Wilson, 1982). This principally means that, during aerobiosis or anaerobiosis, ATP synthesis by metabolism only occurs because ATP has been cleaved and NADH is only ox ...
Metabolic Flux Analysis on the Production of Poly(3 - Wiley-VCH
Metabolic Flux Analysis on the Production of Poly(3 - Wiley-VCH

... analyze the system using the MFA technique, the system should be determined or over-determined, which means that the number of constraints is equal to or greater than that of the reactions. To analyze the under-determined system, more constraints are required. This is often replaced by setting up an ...
- Journal of Hepatology
- Journal of Hepatology

... metabolites (say, <1.5 kDa, to include the larger phospholipid species, such as cardiolipins). Metabolomics in its practice combines high-throughput analytical chemistry, typically, methodologies based upon mass spectrometry or nuclear magnetic resonance spectroscopy, with multivariate data analysis ...
Determination of amino acid enantiomers in human urine and blood
Determination of amino acid enantiomers in human urine and blood

... absolute amounts among D-AAs were determined for D-Ser, in one sample D-Ser and D-Ala were about equal, and in one sample D-Ala was about twice the amount of D-Ser. Quantities of D-Ser ranged from 64 to 199 mmol/ day, of D-Ala from 24 to 138 mmol/day, and of D-Lys from 7 to 94 mmol/L. Relative amoun ...
Unexpected similarities between the
Unexpected similarities between the

... blood metabolome comparison is MTBLS87, while that for metabolomic samples of a single individual’s blood donated 4 times within 24 h to determine RBC : plasma metabolite distribution is MTBLS88. See DOI: 10.1039/c4mb00346b b ...
2 hours
2 hours

... pathways. Understanding the logic of catabolic and anabolic paths, and knowing common molecules (such as ATP) and mechanisms (oxidation-reduction) makes it simpler to understand the myriad paths of metabolism. K. Which of the metabolic chemical reactions is most commonly used to break down foodstuff ...
Graphics are used with permission of
Graphics are used with permission of

... • You've seen the effect that altered pH has on proteins in the body. The systemic effects of ketoacidosis can be dramatic. • The individual with ketoacidosis may experience central nervous system depression, heart dysrhythmias, and decreased cardiac contractility as a result of increased intracellu ...
adam.com (http://www.adam.com/) Benjamin/Cummings Publishing
adam.com (http://www.adam.com/) Benjamin/Cummings Publishing

... • You've seen the effect that altered pH has on proteins in the body. The systemic effects of ketoacidosis can be dramatic. ...
Acid/Base Homeostasis - Interactive Physiology
Acid/Base Homeostasis - Interactive Physiology

... • You've seen the effect that altered pH has on proteins in the body. The systemic effects of ketoacidosis can be dramatic. ...
MS Word Version
MS Word Version

... • You've seen the effect that altered pH has on proteins in the body. The systemic effects of ketoacidosis can be dramatic. • The individual with ketoacidosis may experience central nervous system depression, heart dysrhythmias, and decreased cardiac contractility as a result of increased intracellu ...
MS Word Version - Interactive Physiology
MS Word Version - Interactive Physiology

... • You've seen the effect that altered pH has on proteins in the body. The systemic effects of ketoacidosis can be dramatic. • The individual with ketoacidosis may experience central nervous system depression, heart dysrhythmias, and decreased cardiac contractility as a result of increased intracellu ...
Treating heart attack with different food substrates
Treating heart attack with different food substrates

... reported19,20 and is partly a consequence of a shift in cardiac gene expression from the postnatal pattern to a fetal pattern.16 Some controversy exists as to whether the shift towards glucose use is adaptive or maladaptive. In the rapid pacing canine model of heart failure, in contrast to models of ...
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Pharmacometabolomics

Pharmacometabolomics, also known as pharmacometabonomics, is a field which stems from metabolomics, the quantification and analysis of metabolites produced by the body. It refers to the direct measurement of metabolites in an individual’s bodily fluids, in order to predict or evaluate the metabolism of pharmaceutical compounds, and to better understand the pharmacokinetic profile of a drug. Alternatively, pharmacometabolomics can be applied to measure metabolite levels following the administration of a pharmaceutical compound, in order to monitor the effects of the compound on certain metabolic pathways(pharmacodynamics). This provides detailed mapping of drug effects on metabolism and the pathways that are implicated in mechanism of variation of response to treatment. In addition, the metabolic profile of an individual at baseline (metabotype) provides information about how individuals respond to treatment and highlights heterogeneity within a disease state. All three approaches require the quantification of metabolites found in bodily fluids and tissue, such as blood or urine, and can be used in the assessment of pharmaceutical treatment options for numerous disease states.
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