deschamp_2009_sequencing
... • Small genomes that are not too complex (repeats, duplications...) • The longer the reads, the better – Targeted Resequencing • Complex genomes (crops) – Reduced representation libraries (methyl-sensitive enzymes) ...
... • Small genomes that are not too complex (repeats, duplications...) • The longer the reads, the better – Targeted Resequencing • Complex genomes (crops) – Reduced representation libraries (methyl-sensitive enzymes) ...
Slide 1
... • GWA (Genome-wide Association Studies) have been able to explain only a small amount of heritability ...
... • GWA (Genome-wide Association Studies) have been able to explain only a small amount of heritability ...
Biology 1/e
... 1/4 homozygous dominant AA 1/4 homozygous dominant DD 2/4 heterozygous Aa 2/4 heterozygous Dd 1/4 homozygous recessive aa 1/4 homozygous recessive dd ...
... 1/4 homozygous dominant AA 1/4 homozygous dominant DD 2/4 heterozygous Aa 2/4 heterozygous Dd 1/4 homozygous recessive aa 1/4 homozygous recessive dd ...
Features and phylogeny of the six compared Plasmodium genomes
... Accurate definition of genetic differences between Plasmodium species requires correctly annotated gene models. Therefore, efforts have been taken in this study to both improve the quality of gene models. Since the initial publication of the P. falciparum genome sequence in 2002, large efforts have ...
... Accurate definition of genetic differences between Plasmodium species requires correctly annotated gene models. Therefore, efforts have been taken in this study to both improve the quality of gene models. Since the initial publication of the P. falciparum genome sequence in 2002, large efforts have ...
shERWOOD-UltramiR shRNA
... screening reagent. Equimolar pooling limits shRNA drop out and biased results, while new generation designs provide robust knockdown allowing more consistent and sensitive hit detection. Lentiviral pooled screening libraries are available targeting the whole genome, gene families, pathways or your c ...
... screening reagent. Equimolar pooling limits shRNA drop out and biased results, while new generation designs provide robust knockdown allowing more consistent and sensitive hit detection. Lentiviral pooled screening libraries are available targeting the whole genome, gene families, pathways or your c ...
CNVs vs. SNPs: Understanding Human Structural Variation in Disease
... So, in 2007, Science Magazine announced its breakthrough of the year as human genetic variation. Part of the reason for this was the fact that there were a lot of associations being made between these single nucleotide polymorphisms or SNPs with human diseases. Currently, i ...
... So, in 2007, Science Magazine announced its breakthrough of the year as human genetic variation. Part of the reason for this was the fact that there were a lot of associations being made between these single nucleotide polymorphisms or SNPs with human diseases. Currently, i ...
Number 52, 2005 11 Robert L. M etzenberg
... to mus-51. (These exceptions will be dealt with later.) his-3; mus-51 a (FGSC #9538) - (normal sequence) is transformed to hygromycin resistance with the yfg knockout cassette (but obviously will still require histidine, and they must be held under selection with hygromycin). This strain carries mus ...
... to mus-51. (These exceptions will be dealt with later.) his-3; mus-51 a (FGSC #9538) - (normal sequence) is transformed to hygromycin resistance with the yfg knockout cassette (but obviously will still require histidine, and they must be held under selection with hygromycin). This strain carries mus ...
Paper - Ran Blekhman
... 5.3Mb of human sequence which were divided into 6712 ‘‘case’’ regions. The genomic locations of the TFBSs and ChIP-chip sequences are shown in Figure 1. Using parsimony, we estimated rates of nucleotide substitution at non-CpG-prone sites in the TFBSs, their flanking sequences, the ChIP-chip sequenc ...
... 5.3Mb of human sequence which were divided into 6712 ‘‘case’’ regions. The genomic locations of the TFBSs and ChIP-chip sequences are shown in Figure 1. Using parsimony, we estimated rates of nucleotide substitution at non-CpG-prone sites in the TFBSs, their flanking sequences, the ChIP-chip sequenc ...
The DNA sequence and biology of human chromosome 19
... Markers from the deCODE genetic map were aligned to the chromosome and the average recombination rate was calculated for each 1 Mb window along its length. Female, male, and sex-averaged recombination rates are indicated in pink, blue and yellow, respectively. [Adobe PDF, 528 KB] ...
... Markers from the deCODE genetic map were aligned to the chromosome and the average recombination rate was calculated for each 1 Mb window along its length. Female, male, and sex-averaged recombination rates are indicated in pink, blue and yellow, respectively. [Adobe PDF, 528 KB] ...
Polygenic inheritance and micro/minisatellites
... important role of single base pair changes in a subset of these length variants, (see below and28,40,41), they are consistent with the possibility that both length and sequence can be involved, and that the different sized micro/minisatellite alleles might have an effect on gene function. This hypot ...
... important role of single base pair changes in a subset of these length variants, (see below and28,40,41), they are consistent with the possibility that both length and sequence can be involved, and that the different sized micro/minisatellite alleles might have an effect on gene function. This hypot ...
Xylitol production using recombinant Saccharomyces
... Xylitol production from xylose was studied using recombinant Saccharomyces cere6isiae 2805 containing xylose reductase genes (XYL1 ) of Pichia stipitis at chromosomal d-sequences. S. cere6isiae 2805-39-40, which contains about 40 copies of the XYL1 gene on the chromosome, was obtained by a sequentia ...
... Xylitol production from xylose was studied using recombinant Saccharomyces cere6isiae 2805 containing xylose reductase genes (XYL1 ) of Pichia stipitis at chromosomal d-sequences. S. cere6isiae 2805-39-40, which contains about 40 copies of the XYL1 gene on the chromosome, was obtained by a sequentia ...
DHPS-WDR83 overlapping SNPs detection in QTL region for meat pH
... Among them we detected 353 UniGene clusters containing SNPs. On the whole 617 SNPs were found and, after removing those separated by less than 80 nucleotides, the remaining ones were 379 that decreased to 356 after checking their suitability to be used on the GoldenGate system with a score >0.6 (des ...
... Among them we detected 353 UniGene clusters containing SNPs. On the whole 617 SNPs were found and, after removing those separated by less than 80 nucleotides, the remaining ones were 379 that decreased to 356 after checking their suitability to be used on the GoldenGate system with a score >0.6 (des ...
Microbiology
... Restriction analysis indicated a high degree of similarity to a D N A fragment containing the endo-l,4-P-glucanase gene from B. szlbtilis PAPl 15. The nucleotide sequences of the eglS gene and its flanking regions were determined. Two ORFs were found within a stretch of 3500 bp. One of them, extendi ...
... Restriction analysis indicated a high degree of similarity to a D N A fragment containing the endo-l,4-P-glucanase gene from B. szlbtilis PAPl 15. The nucleotide sequences of the eglS gene and its flanking regions were determined. Two ORFs were found within a stretch of 3500 bp. One of them, extendi ...
Biology 321 Spring 2013 Assignment Set #4 Problems sorted by type
... organism, but, of course, was not as straightforward with humans. You are a genetics graduate student in 1910 and are anxious to determine if the two gene interpretation is correct. In order resolve this issue, you start surveying two generation pedigrees in which the blood groups of the parents and ...
... organism, but, of course, was not as straightforward with humans. You are a genetics graduate student in 1910 and are anxious to determine if the two gene interpretation is correct. In order resolve this issue, you start surveying two generation pedigrees in which the blood groups of the parents and ...
Repeat mediated gene duplication in the Drosophila
... sequences flanking DNA duplications and those found at the breakpoints of chromosomal inversions suggests a connection between these two events. In Drosophila, duplicated genes near inversion breakpoints can arise via unequal genetic exchange during the non-allelic crossing over event giving rise to ...
... sequences flanking DNA duplications and those found at the breakpoints of chromosomal inversions suggests a connection between these two events. In Drosophila, duplicated genes near inversion breakpoints can arise via unequal genetic exchange during the non-allelic crossing over event giving rise to ...
1. The PERL script to
... Introduction Microarray results were analysed for pathway information in the KEGG database using gene names of genes on the microarray. Genes may be known with different names. Therefore, the Gene Ontology database was searched before the KEGG database to collect all possible synonyms of gene names. ...
... Introduction Microarray results were analysed for pathway information in the KEGG database using gene names of genes on the microarray. Genes may be known with different names. Therefore, the Gene Ontology database was searched before the KEGG database to collect all possible synonyms of gene names. ...
"Using Model Organism Databases (MODs)". In: Current - SGD-Wiki
... from them cannot all be covered in this unit. The aim of this set of protocols is to provide a general introduction to enable the novice user to gain entry into various characteristic MODs, then find and retrieve basic information about genes. The unit will explain simple uses for two tools found at ...
... from them cannot all be covered in this unit. The aim of this set of protocols is to provide a general introduction to enable the novice user to gain entry into various characteristic MODs, then find and retrieve basic information about genes. The unit will explain simple uses for two tools found at ...
Studies of the Growth Hormone-Prolactin Gene Family and their
... increases the probability of misalignment between non-homologous segments. Duplicates produced through non-homologous alignment during recombination typically range between a few and 75kb and occur as frequently as 0.002-o.o2 times per gene per million years in eukaryotes (Lynch and Conery 2000). Th ...
... increases the probability of misalignment between non-homologous segments. Duplicates produced through non-homologous alignment during recombination typically range between a few and 75kb and occur as frequently as 0.002-o.o2 times per gene per million years in eukaryotes (Lynch and Conery 2000). Th ...
clinchem.org - Clinical Chemistry
... and spanning a region of 2.4 million bp of genomic DNA, is the largest known human gene (9, 10 ). Approximately 55%– 65% of DMD cases are associated with large intragenic deletions or duplication, ⬃5%–10% with duplications of large segments (11 ), and the remaining cases with point variations, small ...
... and spanning a region of 2.4 million bp of genomic DNA, is the largest known human gene (9, 10 ). Approximately 55%– 65% of DMD cases are associated with large intragenic deletions or duplication, ⬃5%–10% with duplications of large segments (11 ), and the remaining cases with point variations, small ...
Conservation, relocation and duplication in genome evolution
... housekeeping genes [19]. In view of the variety of explanations for the clustering of genes with similar expression levels, it seems an understatement to describe the situation as unsettled. Single-copy genes and multigene families In C. elegans, genes lacking paralogous copies are more likely to ha ...
... housekeeping genes [19]. In view of the variety of explanations for the clustering of genes with similar expression levels, it seems an understatement to describe the situation as unsettled. Single-copy genes and multigene families In C. elegans, genes lacking paralogous copies are more likely to ha ...
Copy-number variation
Copy-number variations (CNVs)—a form of structural variation—are alterations of the DNA of a genome that results in the cell having an abnormal or, for certain genes, a normal variation in the number of copies of one or more sections of the DNA. CNVs correspond to relatively large regions of the genome that have been deleted (fewer than the normal number) or duplicated (more than the normal number) on certain chromosomes. For example, the chromosome that normally has sections in order as A-B-C-D might instead have sections A-B-C-C-D (a duplication of ""C"") or A-B-D (a deletion of ""C"").This variation accounts for roughly 13% of human genomic DNA and each variation may range from about one kilobase (1,000 nucleotide bases) to several megabases in size. CNVs contrast with single-nucleotide polymorphisms (SNPs), which affect only one single nucleotide base.