Chapter 11 Notes
... 2. Another enzyme, DNA polymerase attaches free nucleotides to their complementary base pair by hydrogen bonding. 3. Because this is directional, the pairing of bases must occur in a 5’ to 3’ direction. ...
... 2. Another enzyme, DNA polymerase attaches free nucleotides to their complementary base pair by hydrogen bonding. 3. Because this is directional, the pairing of bases must occur in a 5’ to 3’ direction. ...
genetics i - Indian School Al Wadi Al Kabir
... 1. List the salient features of double helix structure of DNA. 2. (a) In the eukaryotes the DNA molecules are organized within the nucleus. How is the DNA molecule organized in a bacterial cell in absence of a nucleus? (b) Explain the packaging of DNA in eukaryotes. 3. Why is DNA considered a better ...
... 1. List the salient features of double helix structure of DNA. 2. (a) In the eukaryotes the DNA molecules are organized within the nucleus. How is the DNA molecule organized in a bacterial cell in absence of a nucleus? (b) Explain the packaging of DNA in eukaryotes. 3. Why is DNA considered a better ...
PCR Study Questions
... 5. Which characteristic of DNA’s structure contributes most to the ‘melting point’ determination? ...
... 5. Which characteristic of DNA’s structure contributes most to the ‘melting point’ determination? ...
Chapter 12
... 1. What the window look like on a P-20 if you dialed in 6.3 µL? 2. What the window look like on a P-20 if you dialed in 11.7 µL? 3. What the window look like on a P-20 if you dialed in 15.3 µL? 4. What is the purpose of the “first stop” on the micropipettor? 5. What is the purpose of the “second sto ...
... 1. What the window look like on a P-20 if you dialed in 6.3 µL? 2. What the window look like on a P-20 if you dialed in 11.7 µL? 3. What the window look like on a P-20 if you dialed in 15.3 µL? 4. What is the purpose of the “first stop” on the micropipettor? 5. What is the purpose of the “second sto ...
DOC
... After a mismatch is identified and a nick introduced, EXO1 cuts out a section of the DNA strand containing the mismatched base. 7. How do E. coli distinguish between parental and newly replicated strands when performing DNA mismatch repair? For instance, if a T was wrongly paired with a G, how does ...
... After a mismatch is identified and a nick introduced, EXO1 cuts out a section of the DNA strand containing the mismatched base. 7. How do E. coli distinguish between parental and newly replicated strands when performing DNA mismatch repair? For instance, if a T was wrongly paired with a G, how does ...
objective: 1) to describe how the structure of dna allows it to copy itself
... ladder, the helix must first unwind and unzip using an enzyme called DNA helicase ...
... ladder, the helix must first unwind and unzip using an enzyme called DNA helicase ...
CST Review PowerPoint
... functioning of all known living organisms and some viruses. -The DNA segments that carry this genetic information are called genes, but other DNA sequences have structural purposes, or are involved in regulating the use of this genetic information. ...
... functioning of all known living organisms and some viruses. -The DNA segments that carry this genetic information are called genes, but other DNA sequences have structural purposes, or are involved in regulating the use of this genetic information. ...
Genetic Exchange - Pennsylvania State University
... Self-replicative recombination •Transposon or IS self-replicates copy to splice into DNA at a specific target sequences. • Endonuclease activity cuts target sequence, leaving single strand overhanging ends. •Transposon is ligated to ends. • Gaps are filled by DNA polymerase to yield a target sequen ...
... Self-replicative recombination •Transposon or IS self-replicates copy to splice into DNA at a specific target sequences. • Endonuclease activity cuts target sequence, leaving single strand overhanging ends. •Transposon is ligated to ends. • Gaps are filled by DNA polymerase to yield a target sequen ...
Models for homologous recombination
... In bacteria, the major biological role of homologous recombination is to repair DSBs In addition to repairing DSBs in chromosomal DNA, homologous recombination promotes genetic exchange in bacteria In eukaryotic cells, homologous recombination is critical for repairing DNA ...
... In bacteria, the major biological role of homologous recombination is to repair DSBs In addition to repairing DSBs in chromosomal DNA, homologous recombination promotes genetic exchange in bacteria In eukaryotic cells, homologous recombination is critical for repairing DNA ...
Introduction to Agriculture, Food, and Natural Resources
... DNA bases always combine as follows: • Cytosine (C) combines with Guanine (G) • Adenine (A) combines with Thymine (T) ...
... DNA bases always combine as follows: • Cytosine (C) combines with Guanine (G) • Adenine (A) combines with Thymine (T) ...
12.2 DNA Replication ppt
... Action: Adds new nucleotides to the exposed bases using the base-pair rule; also proofreads every connection at this time (avg. 1 error per 2 billion nucleotides) Result: 2 new double DNA strands are created (but still attached) ...
... Action: Adds new nucleotides to the exposed bases using the base-pair rule; also proofreads every connection at this time (avg. 1 error per 2 billion nucleotides) Result: 2 new double DNA strands are created (but still attached) ...
Molecular Basis of Inheritance
... the sugar and phosphate groups (backbone structure) and variable nucleotide bases capable of Hydrogen bonding Conserved region ...
... the sugar and phosphate groups (backbone structure) and variable nucleotide bases capable of Hydrogen bonding Conserved region ...
a copy of the Candy DNA Replication
... Clean all working surfaces and your hands before starting this activity. Your goal is to design a Powerpoint project (or a movie if you know how) that depicts all of the steps of DNA replication. Take photographs of each step and be sure they are easy to see on the Powerpoint. Include labels, arrows ...
... Clean all working surfaces and your hands before starting this activity. Your goal is to design a Powerpoint project (or a movie if you know how) that depicts all of the steps of DNA replication. Take photographs of each step and be sure they are easy to see on the Powerpoint. Include labels, arrows ...
No Slide Title
... • Enables rearrangement of antibody genes • Can lead to cancers - example: Burkitt’s lymphoma caused by translocation between ch. 8 and 14 ...
... • Enables rearrangement of antibody genes • Can lead to cancers - example: Burkitt’s lymphoma caused by translocation between ch. 8 and 14 ...
File - Chereese Langley
... The second of Chargaff's rules (or "Chargaff's second parity rule") is that the composition of DNA varies from one species to another; in particular in the relative amounts of A, G, T, and C bases. Such evidence of molecular diversity, which had been presumed absent from DNA, made DNA a more credib ...
... The second of Chargaff's rules (or "Chargaff's second parity rule") is that the composition of DNA varies from one species to another; in particular in the relative amounts of A, G, T, and C bases. Such evidence of molecular diversity, which had been presumed absent from DNA, made DNA a more credib ...
molecular genetics unit review
... iii. DNA mRNA polypeptide/protein (know how to transcribe DNA and translate mRNA if given a sequence) What are the four ways gene expression is controlled? What is an operon? Describe/explain the 2 main operons (lac, trp) in prokaryotic cells. a) What are mutations? b) What are the different typ ...
... iii. DNA mRNA polypeptide/protein (know how to transcribe DNA and translate mRNA if given a sequence) What are the four ways gene expression is controlled? What is an operon? Describe/explain the 2 main operons (lac, trp) in prokaryotic cells. a) What are mutations? b) What are the different typ ...
Document
... stabilized by RuvAB binding. Pathway A: (A1) RuvC resolves the RuvAB-bound junction. (A2) RecBCD binds to the double-stranded end. (A3) The double-stranded break is repaired by RecBCD/RecAmediated homologous recombination. If the same strands are exchanged at both Holliday junctions, (patch type of ...
... stabilized by RuvAB binding. Pathway A: (A1) RuvC resolves the RuvAB-bound junction. (A2) RecBCD binds to the double-stranded end. (A3) The double-stranded break is repaired by RecBCD/RecAmediated homologous recombination. If the same strands are exchanged at both Holliday junctions, (patch type of ...
Microbiology Unit 3 Study Guide
... 11. Which term describes uneven ends resulting from a restriction enzyme’s cut? 12. Which term describes something that is used to transport genetic material into a target organism? 13. What are the two most commonly used vectors for getting DNA into organisms? 14. How does replication of a bacteria ...
... 11. Which term describes uneven ends resulting from a restriction enzyme’s cut? 12. Which term describes something that is used to transport genetic material into a target organism? 13. What are the two most commonly used vectors for getting DNA into organisms? 14. How does replication of a bacteria ...
DNA-drug interactions and charge transfer processes in DNA.
... Some organic molecules can bind to DNA and thus interfere with DNA replication, transcription and gene expression process, or even direct nucleic acid cleavage. These small molecules can thus act as therapeutic agents in cancer cure. These drug molecules can bind to DNA by different mechanisms. The ...
... Some organic molecules can bind to DNA and thus interfere with DNA replication, transcription and gene expression process, or even direct nucleic acid cleavage. These small molecules can thus act as therapeutic agents in cancer cure. These drug molecules can bind to DNA by different mechanisms. The ...
Name Ch 12 Study Guide
... 10) Assume that the two parent strands of DNA have been separated and that the base sequence on one parent strand is A-T-T-C-G-C; the base sequence that will complement that parent strand is __________________________________________ 11) Who was Rosalind Franklin? 12) What was her contribution to th ...
... 10) Assume that the two parent strands of DNA have been separated and that the base sequence on one parent strand is A-T-T-C-G-C; the base sequence that will complement that parent strand is __________________________________________ 11) Who was Rosalind Franklin? 12) What was her contribution to th ...
Structure of DNA
... • All organisms have a genetic code • DNA (deoxyribonucleic acid) contains the genetic code, and is the blueprint for the expression of physiological traits • Each individual organism has its own unique blueprint ...
... • All organisms have a genetic code • DNA (deoxyribonucleic acid) contains the genetic code, and is the blueprint for the expression of physiological traits • Each individual organism has its own unique blueprint ...
... dna replication is necessary for the transmission of genetic information and thus such a process must achieve accurate copying of the genome. Since the last century the replicon model has been proposed in order to explain the general mechanism of genome duplication in bacteria. Later work in yeast l ...
Reading Guide
... 7. What is the major structural difference between a nucleotide and a deoxynucleotide? What is the major structural difference between DNA and RNA? What is the major functional difference between DNA and RNA? 8. True or false: GC rich DNA strands are harder to separate because GC pairs form more Hbo ...
... 7. What is the major structural difference between a nucleotide and a deoxynucleotide? What is the major structural difference between DNA and RNA? What is the major functional difference between DNA and RNA? 8. True or false: GC rich DNA strands are harder to separate because GC pairs form more Hbo ...
Holliday junction
A Holliday junction is a branched nucleic acid structure that contains four double-stranded arms joined together. These arms may adopt one of several conformations depending on buffer salt concentrations and the sequence of nucleobases closest to the junction. The structure is named after the molecular biologist Robin Holliday, who proposed its existence in 1964.In biology, Holliday junctions are a key intermediate in many types of genetic recombination, as well as in double-strand break repair. These junctions usually have a symmetrical sequence and are thus mobile, meaning that the four individual arms may slide though the junction in a specific pattern that largely preserves base pairing. Additionally, four-arm junctions similar to Holliday junctions appear in some functional RNA molecules.Immobile Holliday junctions, with asymmetrical sequences that lock the strands in a specific position, were artificially created by scientists to study their structure as a model for natural Holliday junctions. These junctions also later found use as basic structural building blocks in DNA nanotechnology, where multiple Holliday junctions can be combined into specific designed geometries that provide molecules with a high degree of structural rigidity.