LAB 3 Bacterial Staining Techniques II I. Differential Stains: Gram
... commonly used in the microbiology laboratory that differentiates bacteria on the basis of their cell wall structure. Most bacteria can be divided into two groups based on the composition of their cell wall: 1) Gram-positive cell walls have a thick peptidoglycan layer beyond the plasma membrane. Char ...
... commonly used in the microbiology laboratory that differentiates bacteria on the basis of their cell wall structure. Most bacteria can be divided into two groups based on the composition of their cell wall: 1) Gram-positive cell walls have a thick peptidoglycan layer beyond the plasma membrane. Char ...
Symbionts and Pathogens: What is the Difference?
... is often difficult to distinguish one from another since a single association may have positive or negative depending on the environmental circumstances. The partners that establish a symbiotic association are called host and symbiont. The host organism is defined as the provider of resources or the ...
... is often difficult to distinguish one from another since a single association may have positive or negative depending on the environmental circumstances. The partners that establish a symbiotic association are called host and symbiont. The host organism is defined as the provider of resources or the ...
Regulation of OmpA and Its Effect on Shigella Virulence
... contains the ribosomal binding sequence (RBS). In order for translation to occur, a ribosome must be able to bind directly to the RBS (Figure 6). RNA thermometers are able to control the binding of a ribosome to the RBS by altering mRNA structure (Figure 7). When the environmental temperature is coo ...
... contains the ribosomal binding sequence (RBS). In order for translation to occur, a ribosome must be able to bind directly to the RBS (Figure 6). RNA thermometers are able to control the binding of a ribosome to the RBS by altering mRNA structure (Figure 7). When the environmental temperature is coo ...
Microbiology of Kitchen Sponges
... Journal of Applied Microbiology. 83(6): 737-750. Sharp, K., and Walker, H., 2003. A microbiological survey of communal kitchens used by undergraduate students. International Journal of ...
... Journal of Applied Microbiology. 83(6): 737-750. Sharp, K., and Walker, H., 2003. A microbiological survey of communal kitchens used by undergraduate students. International Journal of ...
Interference of Staphylococcus aureus virulence factors in the blood
... blood coagulation to their advantage. Staphylococcus aureus is one of these microorganisms. S. aureus is a bacterium that causes a multitude of infections like wound infections but also lethal diseases such as meningitis and sepsis. Many people get infected with this bacteria. In fact, about 90% of ...
... blood coagulation to their advantage. Staphylococcus aureus is one of these microorganisms. S. aureus is a bacterium that causes a multitude of infections like wound infections but also lethal diseases such as meningitis and sepsis. Many people get infected with this bacteria. In fact, about 90% of ...
The antimicrobial resistance pattern of cultured human
... Conclusions Human methanogenic archaea are highly resistant to antibiotics, being susceptible only to molecules that are also effective against both bacteria and eukarya. Methanogenic archaea are good candidates to test for antimicrobial activity against members of this unique domain of life. Furthe ...
... Conclusions Human methanogenic archaea are highly resistant to antibiotics, being susceptible only to molecules that are also effective against both bacteria and eukarya. Methanogenic archaea are good candidates to test for antimicrobial activity against members of this unique domain of life. Furthe ...
Drosophila Immunity: Analysis of PGRP-SB1 Expression, Enzymatic Activity and Function
... In the last decades, peptidoglycan (PGN), an essential component of virtually all bacteria, has appeared as a key player in hostmicroorganism interactions [1]. This glycopeptidic polymer is a component of the cell wall of both Gram-negative and Grampositive bacteria. It consists of long glycan chain ...
... In the last decades, peptidoglycan (PGN), an essential component of virtually all bacteria, has appeared as a key player in hostmicroorganism interactions [1]. This glycopeptidic polymer is a component of the cell wall of both Gram-negative and Grampositive bacteria. It consists of long glycan chain ...
Processing of lysozyme at distinct loops by pepsin: A novel action for
... catalytic residue aspartic acid 52 was substituted with a serine residue [15]. A further attempt to elucidate the structurerelated antimicrobial action of cLZ was recently made in our laboratory with a series of synthetic peptides corresponding to sequences from human and chicken cLZ [4,16]. We foun ...
... catalytic residue aspartic acid 52 was substituted with a serine residue [15]. A further attempt to elucidate the structurerelated antimicrobial action of cLZ was recently made in our laboratory with a series of synthetic peptides corresponding to sequences from human and chicken cLZ [4,16]. We foun ...
AP Biology - Al Young Studios
... Nutritional and Metabolic Diversity 13. Distinguish between photoautotrophs, chemoautotrophs, photoheterotrophs, chemoheterotrophs, saprobes, and parasites. Give examples of each. 14. Describe the process and explain the significance of nitrogen fixation. 15. Distinguish among obligate aerobes, facu ...
... Nutritional and Metabolic Diversity 13. Distinguish between photoautotrophs, chemoautotrophs, photoheterotrophs, chemoheterotrophs, saprobes, and parasites. Give examples of each. 14. Describe the process and explain the significance of nitrogen fixation. 15. Distinguish among obligate aerobes, facu ...
Glucansucrases: mechanism of action and structure–function
... acid bacteria (Lactococci, Lactobacilli). They catalyse the synthesis of high molecular weight D-glucose polymers, named glucans, from sucrose. In the presence of efficient acceptors, they catalyse the synthesis of low molecular weight oligosaccharides. Glucosidic bond synthesis occurs without the m ...
... acid bacteria (Lactococci, Lactobacilli). They catalyse the synthesis of high molecular weight D-glucose polymers, named glucans, from sucrose. In the presence of efficient acceptors, they catalyse the synthesis of low molecular weight oligosaccharides. Glucosidic bond synthesis occurs without the m ...
10470 exploring bacteria - The Described and Captioned Media
... they cannot see. Though tiny, these microscopic bacteria are busily carrying out complex life functions in a stunning variety of habitats. Even inside the students' own intestines, a huge community of bacteria is engaged in getting food, reproducing and fighting off predators. Bacteria provide a ric ...
... they cannot see. Though tiny, these microscopic bacteria are busily carrying out complex life functions in a stunning variety of habitats. Even inside the students' own intestines, a huge community of bacteria is engaged in getting food, reproducing and fighting off predators. Bacteria provide a ric ...
HEMOSTASIS
... A number of GPs present in this area are responsible for blood group specificity (ABO), tissue compatibility (HLA), and platelet antigenicity. Platelet membrane glyco-proteins serve as a receptor and facilitate transmission of stimuli across the platelet membrane. Platelet membrane GPIb appears to b ...
... A number of GPs present in this area are responsible for blood group specificity (ABO), tissue compatibility (HLA), and platelet antigenicity. Platelet membrane glyco-proteins serve as a receptor and facilitate transmission of stimuli across the platelet membrane. Platelet membrane GPIb appears to b ...
Determination of the Gram Type Using the Reaction
... medium with peptone, yeast extract and glucose was employed. Incubation withpolymyxin B. Bacteria were suspended in 1 ml10 mM-Tris/HClbuffer, pH 7.2, which, in the case of halophilic bacteria, was supplemented with up to 25% (w/v) NaCl to prevent lysis. Samples (0.25 ml) of this suspension were incu ...
... medium with peptone, yeast extract and glucose was employed. Incubation withpolymyxin B. Bacteria were suspended in 1 ml10 mM-Tris/HClbuffer, pH 7.2, which, in the case of halophilic bacteria, was supplemented with up to 25% (w/v) NaCl to prevent lysis. Samples (0.25 ml) of this suspension were incu ...
Bakteriell fysiologi och patogenes, 7.5 hp
... - Give examples of the principles behind transcriptional , post-transcriptional , translational and posttranslational bacterial gene regulatory mechanisms and their linkage to changes in the environment - Summarize the bioenergetics of bacteria , including electron transport and how proton motif for ...
... - Give examples of the principles behind transcriptional , post-transcriptional , translational and posttranslational bacterial gene regulatory mechanisms and their linkage to changes in the environment - Summarize the bioenergetics of bacteria , including electron transport and how proton motif for ...
Determination of the Gram Type Using the Reaction Between
... medium with peptone, yeast extract and glucose was employed. Incubation withpolymyxin B. Bacteria were suspended in 1 ml10 mM-Tris/HClbuffer, pH 7.2, which, in the case of halophilic bacteria, was supplemented with up to 25% (w/v) NaCl to prevent lysis. Samples (0.25 ml) of this suspension were incu ...
... medium with peptone, yeast extract and glucose was employed. Incubation withpolymyxin B. Bacteria were suspended in 1 ml10 mM-Tris/HClbuffer, pH 7.2, which, in the case of halophilic bacteria, was supplemented with up to 25% (w/v) NaCl to prevent lysis. Samples (0.25 ml) of this suspension were incu ...
Medical Microbiology Microscopic slides and media
... Class 5. Oral microbiology – Bacteria causing inflammations and abscesses within the oral cavity; Gram-positive facultative cocci (genus Staphylococcus, Streptococcus and Enterococcus). Identification of staphylococci in selective-differential medium – mannitol salt agar (Chapman’s medium). Mannito ...
... Class 5. Oral microbiology – Bacteria causing inflammations and abscesses within the oral cavity; Gram-positive facultative cocci (genus Staphylococcus, Streptococcus and Enterococcus). Identification of staphylococci in selective-differential medium – mannitol salt agar (Chapman’s medium). Mannito ...
Vol. 14 No. 11 - Beneficial Bacteria
... the intestinal microflora is out of balance. Probiotics by themselves help to overcome constipation, too. Finally, about 70% of the energy requirements of the intestinal mucosa come from fatty acids produced as a by product of bacterial fermentation. This means that the intestinal microflora actuall ...
... the intestinal microflora is out of balance. Probiotics by themselves help to overcome constipation, too. Finally, about 70% of the energy requirements of the intestinal mucosa come from fatty acids produced as a by product of bacterial fermentation. This means that the intestinal microflora actuall ...
... Bacteria can be beneficial or harmful. Beneficial bacteria co-exist with us, living in our gut and on our skin, without causing disease. However, even “good” bacteria can become harmful when they find their way to a different part of the body. They may also do harm when they infect someone who has a ...
bacterial investigations lab
... Many antibiotics, including penicillins, inhibit the synthesis of cross-links in peptidoglycans, preventing the formation of a functional wall, particularly in gram-positive species. These drugs are a very selective treatment because they cripple many species of bacteria without affecting humans ...
... Many antibiotics, including penicillins, inhibit the synthesis of cross-links in peptidoglycans, preventing the formation of a functional wall, particularly in gram-positive species. These drugs are a very selective treatment because they cripple many species of bacteria without affecting humans ...
Lipopolysaccharide: Biosynthetic pathway and structure modification Xiaoyuan Wang , Peter J. Quinn Review
... 2.1. Biosynthesis of Kdo2-lipid A E. coli is the most favoured Gram-negative bacterium for studies of LPS biosynthesis. The first stage of the biosynthetic pathway is the synthesis of Kdo2-lipid A [3,13]. The pathway is mediated by nine enzymes (Table 1) and takes place in the cytoplasm and on the in ...
... 2.1. Biosynthesis of Kdo2-lipid A E. coli is the most favoured Gram-negative bacterium for studies of LPS biosynthesis. The first stage of the biosynthetic pathway is the synthesis of Kdo2-lipid A [3,13]. The pathway is mediated by nine enzymes (Table 1) and takes place in the cytoplasm and on the in ...
A Strategy to Identify Novel Antimicrobial Compounds
... strategy for identification of such targets via a bioinformatics approach. In our first study we compared proteins with unknown and hypothetical function of the spirochete Treponema pallidum to five other pathogens also causing chronic or persistent infections in humans (Yersinia pestis, Neisseria g ...
... strategy for identification of such targets via a bioinformatics approach. In our first study we compared proteins with unknown and hypothetical function of the spirochete Treponema pallidum to five other pathogens also causing chronic or persistent infections in humans (Yersinia pestis, Neisseria g ...
Bacteria - HCC Learning Web
... • An important feature of nearly all prokaryotic cells is their cell wall, which maintains cell shape, provides physical protection, and prevents the cell from bursting in a hypotonic environment • Eukaryote cell walls are made of cellulose or chitin • Bacterial cell walls contain peptidoglycan, a n ...
... • An important feature of nearly all prokaryotic cells is their cell wall, which maintains cell shape, provides physical protection, and prevents the cell from bursting in a hypotonic environment • Eukaryote cell walls are made of cellulose or chitin • Bacterial cell walls contain peptidoglycan, a n ...
LIN, a Novel Type of U-Box/WD40 Protein
... identify the LIN gene in Medicago truncatula and Lotus japonicus, showing that it codes for a predicted E3 ubiquitin ligase containing a highly conserved U-box and WD40 repeat domains. Ubiquitin-mediated protein degradation is a universal mechanism to regulate many biological processes by eliminatin ...
... identify the LIN gene in Medicago truncatula and Lotus japonicus, showing that it codes for a predicted E3 ubiquitin ligase containing a highly conserved U-box and WD40 repeat domains. Ubiquitin-mediated protein degradation is a universal mechanism to regulate many biological processes by eliminatin ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)
... aminoglycosides are frequently used for the treatment of such infection but strains of Pseudomonas aeruginosa and other bacterial species resistant to aminoglycoside are now been reported in Nigeria and this is probably because gentamicin and other aminoglycosides is traditionally considered in this ...
... aminoglycosides are frequently used for the treatment of such infection but strains of Pseudomonas aeruginosa and other bacterial species resistant to aminoglycoside are now been reported in Nigeria and this is probably because gentamicin and other aminoglycosides is traditionally considered in this ...
Trimeric autotransporter adhesin
In molecular biology, trimeric autotransporter adhesins (TAAs), are proteins found on the outer membrane of Gram-negative bacteria. Bacteria use TAAs in order to infect their host cells via a process called cell adhesion. TAAs also go by another name, oligomeric coiled-coil adhesins, which is shortened to OCAs. In essence, they are virulence factors, factors that make the bacteria harmful and infective to the host organism.TAAs are just one of many methods bacteria use to infect their hosts, infection resulting in diseases such as pneumonia, sepsis, and meningitis. Most bacteria infect their host through a method named the secretion pathway. TAAs are part of the secretion pathway, to be more specific the type Vc secretion system.Trimeric autotransporter adhesins have a unique structure. The structure they hold is crucial to their function. They all appear to have a head-stalk-anchor structure. Each TAA is made up of three identical proteins, hence the name trimeric. Once the membrane anchor has been inserted into the outer membrane, the passenger domain passes through it into the host extracellular environment autonomously, hence the description of autotransporter. The head domain, once assembled, then adheres to an element of the host extracellular matrix, for example, collagen, fibronectin, etc.