University of Groningen Methionine
... For RNA isolation the following procedure was performed: the pellet of the harvested cells was resuspended in 400 ml TE buffer (diethylpyrocarbonate) and the resuspended cells were added into RNA-free screw-cap tubes containing 0.5 g glass beads, 50 ml 10 % SDS, 500 ml phenol/chloroform: isoamylalco ...
... For RNA isolation the following procedure was performed: the pellet of the harvested cells was resuspended in 400 ml TE buffer (diethylpyrocarbonate) and the resuspended cells were added into RNA-free screw-cap tubes containing 0.5 g glass beads, 50 ml 10 % SDS, 500 ml phenol/chloroform: isoamylalco ...
4 Mapping Eukaryotic Chromosomes by
... not all the same size (frequency)? Answer: Figure 4-14 represents a trihybrid testcross with linked genes, so there are two genetic intervals to consider. In a typical three-point testcross, those intervals will be different sizes with correspondingly different frequencies of SCOs. The colored boxes ...
... not all the same size (frequency)? Answer: Figure 4-14 represents a trihybrid testcross with linked genes, so there are two genetic intervals to consider. In a typical three-point testcross, those intervals will be different sizes with correspondingly different frequencies of SCOs. The colored boxes ...
Repeat mediated gene duplication in the Drosophila
... The D. pseudoobscura genome was partitioned into genic regions (sequences identified as exons or introns in the initial annotation) and intergenic regions (sequence between the genic regions). (The coordinates of the genic and intergenic regions are provided as Supplementary Material.) The genic and ...
... The D. pseudoobscura genome was partitioned into genic regions (sequences identified as exons or introns in the initial annotation) and intergenic regions (sequence between the genic regions). (The coordinates of the genic and intergenic regions are provided as Supplementary Material.) The genic and ...
Supertaster
... same as cupcakes! Not only does taste make consuming food more interesting, but it also serves a purpose – it allows humans to determine the relative safety of what they plan to consume. Toxic substances tend to be bitter in taste, so humans tend to avoid this particular flavor – not only do most pe ...
... same as cupcakes! Not only does taste make consuming food more interesting, but it also serves a purpose – it allows humans to determine the relative safety of what they plan to consume. Toxic substances tend to be bitter in taste, so humans tend to avoid this particular flavor – not only do most pe ...
Carbapenemase and virulence factors of
... Objectives: To investigate the resistance to carbapenems in Enterobacteriaceae and the underlying resistance mechanisms in North Lebanon between 2008 and 2012. Methods: A total of 2767 Enterobacteriaceae isolates recovered from clinical samples collected in Nini Hospital (North Lebanon) were screene ...
... Objectives: To investigate the resistance to carbapenems in Enterobacteriaceae and the underlying resistance mechanisms in North Lebanon between 2008 and 2012. Methods: A total of 2767 Enterobacteriaceae isolates recovered from clinical samples collected in Nini Hospital (North Lebanon) were screene ...
Supplementary Information (doc 408K)
... pipeline. To increase confidence that homozygous calls are correctly distinguished from heterozygous calls, individuals homozygous for the reference and non-reference allele had to pass an allele balance (number of alternative reads/total number of reads) filter of less than 0.1 and greater than 0.9 ...
... pipeline. To increase confidence that homozygous calls are correctly distinguished from heterozygous calls, individuals homozygous for the reference and non-reference allele had to pass an allele balance (number of alternative reads/total number of reads) filter of less than 0.1 and greater than 0.9 ...
The photosynthetic apparatus of Prochlorococcus
... system in the high B/A ecotypes may support photosynthesis in these genotypes under very low irradiances, i.e. those receiving only about 0.21 % surface PAR (Johnson et al., 1999). The presence of phycourobilin as the dominating chromophore (Hess et al., 1996) is in agreement with such a scenario as ...
... system in the high B/A ecotypes may support photosynthesis in these genotypes under very low irradiances, i.e. those receiving only about 0.21 % surface PAR (Johnson et al., 1999). The presence of phycourobilin as the dominating chromophore (Hess et al., 1996) is in agreement with such a scenario as ...
Resistance to cephalosporins and carbapenems in Gram
... have firstly been described in India, there is no real evidence for spreading to UK and France later on. Further more, it remains unclear why CTX-M-group 9 enzymes (CTX-M-14, CTX-M-16, CTXM-19, CTX-M-27) are predominant in Spain and group 1 enzymes (CTX-M-1, CTX-M-3, CTX-M-15) in most of the other Eu ...
... have firstly been described in India, there is no real evidence for spreading to UK and France later on. Further more, it remains unclear why CTX-M-group 9 enzymes (CTX-M-14, CTX-M-16, CTXM-19, CTX-M-27) are predominant in Spain and group 1 enzymes (CTX-M-1, CTX-M-3, CTX-M-15) in most of the other Eu ...
ABSTRACT Using a bioinformatics approach to identify genes that
... categorizations. While public users can enter a gene set into GeneWeaver, most gene sets are derived directly from experimentation (GWAS, Microarray, etc.) or publications. For example, PubMed, a database for scientific literature, can be used to find articles that describe gene sets, and these gene ...
... categorizations. While public users can enter a gene set into GeneWeaver, most gene sets are derived directly from experimentation (GWAS, Microarray, etc.) or publications. For example, PubMed, a database for scientific literature, can be used to find articles that describe gene sets, and these gene ...
Expansion of the Pseudo-autosomal Region and Ongoing
... available in the human PAR1 (Lien et al. 2000), despite the progress towards a complete genome sequence (International Human Genome Sequencing Consortium 2001). Eutherian PARs now include only a few genes (e.g., Van Laere et al. 2008), making it unlikely that SA polymorphisms will be found among the ...
... available in the human PAR1 (Lien et al. 2000), despite the progress towards a complete genome sequence (International Human Genome Sequencing Consortium 2001). Eutherian PARs now include only a few genes (e.g., Van Laere et al. 2008), making it unlikely that SA polymorphisms will be found among the ...
Unit 4 - Purdue Agriculture
... two researchers, Francis Crick and James Watson, proposed a double-helix structure for the molecule that we began to understand how this material carries the genetic code of life. A gene is a unit of information that can be passed on to offspring. It is a segment of DNA. Genes help determine whether ...
... two researchers, Francis Crick and James Watson, proposed a double-helix structure for the molecule that we began to understand how this material carries the genetic code of life. A gene is a unit of information that can be passed on to offspring. It is a segment of DNA. Genes help determine whether ...
[Full text/PDF]
... (COPD), which is a heritable multi-factorial disease. We present genome-wide association study (GWAS) to map the genetic architecture of this trait and investigate the networks between the external factors (smoking and gender) and genetic factors. By using a mixed linear model and a conditional mode ...
... (COPD), which is a heritable multi-factorial disease. We present genome-wide association study (GWAS) to map the genetic architecture of this trait and investigate the networks between the external factors (smoking and gender) and genetic factors. By using a mixed linear model and a conditional mode ...
The Chloroplast trnT–trnF Region in the Seed Plant
... et al. 1991). Even where chloroplast genomes have undergone extreme structural changes, such as in Pinus thunbergii (Wakasugi et al. 1994) (Fig. 1B), which has pseudogenized ndhF genes located immediately downstream from the trnT–trnF region, the region itself is conserved. Recent large-scale analys ...
... et al. 1991). Even where chloroplast genomes have undergone extreme structural changes, such as in Pinus thunbergii (Wakasugi et al. 1994) (Fig. 1B), which has pseudogenized ndhF genes located immediately downstream from the trnT–trnF region, the region itself is conserved. Recent large-scale analys ...
Heredity and Development: Second Edition
... soon there were more of them than there were pairs of homologous chromosomes. This was the difficult moment for genetics and cytology that Sutton had predicted (page 78). The Prediction of Linkage. At the time Sutton proposed his hypothesis, he pointed out one situation in which the Mendelian laws c ...
... soon there were more of them than there were pairs of homologous chromosomes. This was the difficult moment for genetics and cytology that Sutton had predicted (page 78). The Prediction of Linkage. At the time Sutton proposed his hypothesis, he pointed out one situation in which the Mendelian laws c ...
Expression of the six chromate ion transporter
... universal primers. DNA fragments containing the chr genes were obtained by digestions with HindIII/XbaI or HindIII/EcoRI endonucleases and subcloned into the corresponding sites of pACYC184 or pUCP20 vectors. E. coli W3110 cells were transformed by electroporation with recombinant plasmids and trans ...
... universal primers. DNA fragments containing the chr genes were obtained by digestions with HindIII/XbaI or HindIII/EcoRI endonucleases and subcloned into the corresponding sites of pACYC184 or pUCP20 vectors. E. coli W3110 cells were transformed by electroporation with recombinant plasmids and trans ...
The amphioxus hairy family: differential fate after duplication.
... but in contrast to its paralog, neither in segmented somites nor in the PSM. Within the CNS, her9 is predominantly expressed in the fore- and midbrain, and transiently in the hindbrain, leaving a non-expressing gap at the midbrain-hindbrain boundary (MHB), and it is also expressed in the midline mes ...
... but in contrast to its paralog, neither in segmented somites nor in the PSM. Within the CNS, her9 is predominantly expressed in the fore- and midbrain, and transiently in the hindbrain, leaving a non-expressing gap at the midbrain-hindbrain boundary (MHB), and it is also expressed in the midline mes ...
More about the. tabby mouse and about the Lyon hypothesis
... validity will have to establish whether the facts claimed to support the hypothesis are indeed in quantitative agreement with its consequences. Methodologically it is obvious that in the first instance this will have to be done in the simplest genetic situations, taking one gene at a time and studyi ...
... validity will have to establish whether the facts claimed to support the hypothesis are indeed in quantitative agreement with its consequences. Methodologically it is obvious that in the first instance this will have to be done in the simplest genetic situations, taking one gene at a time and studyi ...
The Optimal Discovery Procedure II: Applications to Comparative
... A microarray measures the abundance of mRNA transcripts of thousands of genes from a single biological sample (1, 2). Of much recent interest has been the problem of identifying genes that are differentially expressed across varying biological conditions based on microarray data (3). It is now possi ...
... A microarray measures the abundance of mRNA transcripts of thousands of genes from a single biological sample (1, 2). Of much recent interest has been the problem of identifying genes that are differentially expressed across varying biological conditions based on microarray data (3). It is now possi ...
The Chromosomal Basis of Inheritance
... tackled. Mendel chose the garden pea because a number of distinct varieties were available. For his work, Morgan selected a species of fruit fly, Drosophila melanogaster, a common insect that feeds on the fungi growing on fruit. Fruit flies are prolific breeders; a single mating will produce hundreds o ...
... tackled. Mendel chose the garden pea because a number of distinct varieties were available. For his work, Morgan selected a species of fruit fly, Drosophila melanogaster, a common insect that feeds on the fungi growing on fruit. Fruit flies are prolific breeders; a single mating will produce hundreds o ...
Genome-Wide Analysis of In Vivo Binding of the Master Regulator
... To allow activation and screening of cryptic antibiotic biosynthetic clusters, detailed insight is required into the linkages between environmental (nutritional) signals and secondary metabolite production. Carbon source utilization is a major determining factor in the metabolic control of antibioti ...
... To allow activation and screening of cryptic antibiotic biosynthetic clusters, detailed insight is required into the linkages between environmental (nutritional) signals and secondary metabolite production. Carbon source utilization is a major determining factor in the metabolic control of antibioti ...
Dominant and Recessive Genes
... first half of overhead number one (Point A and sub-points 1-3). Through this discussion you should lead students to the Red Angus breed. For the purpose of this lesson B is the dominant gene in cattle that codes for a black coat; b is the recessive form of this allele that codes for a red coat. Usin ...
... first half of overhead number one (Point A and sub-points 1-3). Through this discussion you should lead students to the Red Angus breed. For the purpose of this lesson B is the dominant gene in cattle that codes for a black coat; b is the recessive form of this allele that codes for a red coat. Usin ...
Essential gene
Essential genes are those genes of an organism that are thought to be critical for its survival. However, being essential is highly dependent on the circumstances in which an organism lives. For instance, a gene required to digest starch is only essential if starch is the only source of energy. Recently, systematic attempts have been made to identify those genes that are absolutely required to maintain life, provided that all nutrients are available. Such experiments have led to the conclusion that the absolutely required number of genes for bacteria is on the order of about 250-300. These essential genes encode proteins to maintain a central metabolism, replicate DNA, translate genes into proteins, maintain a basic cellular structure, and mediate transport processes into and out of the cell. Most genes are not essential but convey selective advantages and increased fitness.