Cardiac O-GlcNAc signaling is increased in hypertrophy and heart
... physiological and pathological functions in the cell (17). Although there is a growing recognition that global protein O-GlcNAc alterations are involved in cardiovascular pathophysiology in diabetes and acute ischemia (13, 27, 33, 35, 51), regulation in the heart is currently not well understood. In ...
... physiological and pathological functions in the cell (17). Although there is a growing recognition that global protein O-GlcNAc alterations are involved in cardiovascular pathophysiology in diabetes and acute ischemia (13, 27, 33, 35, 51), regulation in the heart is currently not well understood. In ...
Diversity of heterotrimeric G-protein γ subunits in plants | SpringerLink
... at least two variants of this type related to the Arabidopsis AGG1 and AGG2 proteins. At the same time most asterid species have only one Gγ subunit of this type. The second Gγ subunit type, type B, has a substantial level of amino acid sequence similarity to the type A Gγ subunits, however, lacks t ...
... at least two variants of this type related to the Arabidopsis AGG1 and AGG2 proteins. At the same time most asterid species have only one Gγ subunit of this type. The second Gγ subunit type, type B, has a substantial level of amino acid sequence similarity to the type A Gγ subunits, however, lacks t ...
Influence of bovine serum albumin (BSA)
... in formulation of detergents, food emulsion, pharmaceutical, cosmetic products etc. Also, an increased understanding within this field is found to be of great importance for other related fields such as protein surfactant absorption at interface [2] as well as in relatively complex biological phenom ...
... in formulation of detergents, food emulsion, pharmaceutical, cosmetic products etc. Also, an increased understanding within this field is found to be of great importance for other related fields such as protein surfactant absorption at interface [2] as well as in relatively complex biological phenom ...
A Cyanobacterial Chlorophyll Synthase-HliD
... YidC, and HliD in the FLAG-ChlG eluate, all of which were absent from the control pull-down (Figure 1B). In addition, we detected a specific signal for SecY for the FLAG-ChlG pulldown, as well as a signal for the Rpl1 ribosome subunit that is clearly stronger than in the control (Figure 1B). We also ...
... YidC, and HliD in the FLAG-ChlG eluate, all of which were absent from the control pull-down (Figure 1B). In addition, we detected a specific signal for SecY for the FLAG-ChlG pulldown, as well as a signal for the Rpl1 ribosome subunit that is clearly stronger than in the control (Figure 1B). We also ...
Slide 1
... Vacuoles-are membranous sacs containing proteins and polysaccharides that support specific functions. -come in different shapes, sizes and serve a variety of functions. -some hold food, some store water, some compartmentalize vital chemicals, some concentrate the waste products of cell metabolism. ...
... Vacuoles-are membranous sacs containing proteins and polysaccharides that support specific functions. -come in different shapes, sizes and serve a variety of functions. -some hold food, some store water, some compartmentalize vital chemicals, some concentrate the waste products of cell metabolism. ...
The role of cytosolic proteins in the insertion of tail
... carrier cytochrome b5 (b5) and the protein tyrosine phosphatase (PTP)-1B, are instead able to translocate at nanomolar ATP concentrations into pure lipid vesicles, provided that these have low levels of cholesterol (Brambillasca et al., 2006; Brambillasca et al., 2005). We have called this latter me ...
... carrier cytochrome b5 (b5) and the protein tyrosine phosphatase (PTP)-1B, are instead able to translocate at nanomolar ATP concentrations into pure lipid vesicles, provided that these have low levels of cholesterol (Brambillasca et al., 2006; Brambillasca et al., 2005). We have called this latter me ...
Delivering of Proteins to the Plant Vacuole—An Update
... with their membrane. The VSR is then recycled back to the TGN and the cargo molecules are transported to the vacuole, where the fusion of the PVC/MVB with the tonoplast occurs [15]. As the trafficking to the vacuole can occur by different routes and may involve different intermediates it will be app ...
... with their membrane. The VSR is then recycled back to the TGN and the cargo molecules are transported to the vacuole, where the fusion of the PVC/MVB with the tonoplast occurs [15]. As the trafficking to the vacuole can occur by different routes and may involve different intermediates it will be app ...
Pdf
... secondary structure. As a straightforward implementation of this general idea, we have designed the following highly simplified network. The input representation for each amino acid is a 5-bit binary number ordered to reflect one of the following scales: an a-helix promotion ordering of the amino ac ...
... secondary structure. As a straightforward implementation of this general idea, we have designed the following highly simplified network. The input representation for each amino acid is a 5-bit binary number ordered to reflect one of the following scales: an a-helix promotion ordering of the amino ac ...
Ribosomes and Protein Synthesis
... of Moore and Stein (30) made possible the unequivocal separation of the amino acids in proteins after a "C-labeling experiment. Thus it could be shown that when alanine, glycine, glutamic or aspartic acids are labeled in the carboxyl position with `C and used in a tissue-slice or whole-animal incorp ...
... of Moore and Stein (30) made possible the unequivocal separation of the amino acids in proteins after a "C-labeling experiment. Thus it could be shown that when alanine, glycine, glutamic or aspartic acids are labeled in the carboxyl position with `C and used in a tissue-slice or whole-animal incorp ...
Click Here to download this tutorial as a PDF
... MIME Project by Henry Rzepa. It is similar to .pdb files in that it contains the 3dimensional locations of atoms in a molecular structure. However, unlike .pdb files, .mol files are often used for smaller structures such as ligands, drugs and sugars. There are a large number of .mol file sources inc ...
... MIME Project by Henry Rzepa. It is similar to .pdb files in that it contains the 3dimensional locations of atoms in a molecular structure. However, unlike .pdb files, .mol files are often used for smaller structures such as ligands, drugs and sugars. There are a large number of .mol file sources inc ...
Observations of green fluorescent protein as a fusion partner in
... Fullerton, CA). The total protein assay was performed using a protein assay kit (Bio-Rad Lab., Hercules, CA) with bovine serum albumin as a standard. The GFPuv whole cell assay was performed by measuring fluorescence intensity using a fluorescence spectrometer (LS-3B; Perkin-Elmer Ltd., Beaconsfield ...
... Fullerton, CA). The total protein assay was performed using a protein assay kit (Bio-Rad Lab., Hercules, CA) with bovine serum albumin as a standard. The GFPuv whole cell assay was performed by measuring fluorescence intensity using a fluorescence spectrometer (LS-3B; Perkin-Elmer Ltd., Beaconsfield ...
Functional Characterization of the 180
... A combination of strategies was used to isolate and assemble a full-length clone encoding RRp. A 3.7-kb clone, representing the 3' end of the m R N A , was isolated from an M D C K cell c D N A expression library using a rabbit antiRRp antiserum (Savitz and Meyer, 1990). The primary structure deduce ...
... A combination of strategies was used to isolate and assemble a full-length clone encoding RRp. A 3.7-kb clone, representing the 3' end of the m R N A , was isolated from an M D C K cell c D N A expression library using a rabbit antiRRp antiserum (Savitz and Meyer, 1990). The primary structure deduce ...
Phosphoproteomics reveals extensive in vivo phosphorylation of
... growth in the amount of studies describing the use of immobilized metal affinity chromatography (IMAC) (1) or other phosphopeptide purification methods such as metal oxide affinity chromatography (2) or TiO2 (3) coupled to mass spectrometric analysis. IMAC has been used most successfully for the lar ...
... growth in the amount of studies describing the use of immobilized metal affinity chromatography (IMAC) (1) or other phosphopeptide purification methods such as metal oxide affinity chromatography (2) or TiO2 (3) coupled to mass spectrometric analysis. IMAC has been used most successfully for the lar ...
tethering redox proteins to the outer membrane in Neisseria and
... the capacity for tumbling. The AniA protein is a trimer [9], and thus in N. meningitidis, it is tethered to the outer membrane by three distinct peptides. The enzyme structure approximates to that of a triangular prism with a height of ∼50 Å and triangular sides of ∼70 Å. The three tethering pepti ...
... the capacity for tumbling. The AniA protein is a trimer [9], and thus in N. meningitidis, it is tethered to the outer membrane by three distinct peptides. The enzyme structure approximates to that of a triangular prism with a height of ∼50 Å and triangular sides of ∼70 Å. The three tethering pepti ...
Protein Quality Control along the Route to the Plant Vacuole
... Ceriotti et al., 1991). Such resistance is probably necessary for their efficient accumulation in protein storage vacuoles: in fact, these compartments also accumulate proteolytic enzymes, although their hydrolytic activity is lower than that of vacuoles of vegetative tissues (Okita and Rogers, 1996 ...
... Ceriotti et al., 1991). Such resistance is probably necessary for their efficient accumulation in protein storage vacuoles: in fact, these compartments also accumulate proteolytic enzymes, although their hydrolytic activity is lower than that of vacuoles of vegetative tissues (Okita and Rogers, 1996 ...
Biochemical Origins of Alzheimer`s Disease With Treatment
... rapidly than A40. In patients fifty years or younger it was found that only 6.3% of senile plaques were A40, while in older brains, fifty or older, the amount of A40 increased to 42%5. A42 has two additional hydrophobic amino acids than A40; the presence of isoleucine and alanine increase the ...
... rapidly than A40. In patients fifty years or younger it was found that only 6.3% of senile plaques were A40, while in older brains, fifty or older, the amount of A40 increased to 42%5. A42 has two additional hydrophobic amino acids than A40; the presence of isoleucine and alanine increase the ...
Translation of an integral membrane protein in distal dendrites of
... Maintenance of synaptic plasticity requires protein translation. Because changes in synaptic strength are regulated at the level of individual synapses, a mechanism is required for newly translated proteins to specifically and persistently modify only a subset of synapses. Evidence suggests this may ...
... Maintenance of synaptic plasticity requires protein translation. Because changes in synaptic strength are regulated at the level of individual synapses, a mechanism is required for newly translated proteins to specifically and persistently modify only a subset of synapses. Evidence suggests this may ...
A Survey of Flexible Protein Binding Mechanisms and their
... approach has predicted that more than 30% of the genome of 29 eukaryotes have proteins with disordered regions of 40 or more consecutive residues.34 An energy landscape survey of a large database of protein complexes has suggested that w15% of monomers may not fold in the absence of partner proteins ...
... approach has predicted that more than 30% of the genome of 29 eukaryotes have proteins with disordered regions of 40 or more consecutive residues.34 An energy landscape survey of a large database of protein complexes has suggested that w15% of monomers may not fold in the absence of partner proteins ...
Small-angle scattering studies of intrinsically disordered proteins
... complementary to NMR since the two techniques probe non-overlapping parts of the same entity [68]. Conversely, SAXS probes the complete assembly and can be used to integrate the information from both NMR and X-ray crystallography. If one of the partners is deuterated, contrast variation SANS experi ...
... complementary to NMR since the two techniques probe non-overlapping parts of the same entity [68]. Conversely, SAXS probes the complete assembly and can be used to integrate the information from both NMR and X-ray crystallography. If one of the partners is deuterated, contrast variation SANS experi ...
The Plant Journal
... with the cell plate. ADL2 was localized to the plastids (Kang et al., 1998). ADL6 was shown to associate with the Golgi apparatus (Jin et al., 2001). The cellular compartment to which ADL3 associates is currently unknown. Apart from difference in subcellular localization, the cellular functions of d ...
... with the cell plate. ADL2 was localized to the plastids (Kang et al., 1998). ADL6 was shown to associate with the Golgi apparatus (Jin et al., 2001). The cellular compartment to which ADL3 associates is currently unknown. Apart from difference in subcellular localization, the cellular functions of d ...
Processing Synthesis of Cellulase from Ripening
... Gel Electrophoresis, Protein Blotting, and Immunodetection. Proteins were analyzed by SDS-PAGE using 16 cm 10% polyacrylamide gels and the buffer system of Laemmli (24). For immunodetection of cellulase, proteins were electroblotted to nitrocellulose (Bio-Rad) essentially as described by Burnette (7 ...
... Gel Electrophoresis, Protein Blotting, and Immunodetection. Proteins were analyzed by SDS-PAGE using 16 cm 10% polyacrylamide gels and the buffer system of Laemmli (24). For immunodetection of cellulase, proteins were electroblotted to nitrocellulose (Bio-Rad) essentially as described by Burnette (7 ...
Light-dependent Dl Protein Synthesis and Translocation Is
... synthesis inhibitors. In this procedure the labeling of the Dl protein is effectively blocked (see Figs. 2-4). However, labeling of cytosolic synthesized proteins continues during the chase as shown in Figs. 2-4. Addition of cycloheximide which could have prevented this further labeling was not used ...
... synthesis inhibitors. In this procedure the labeling of the Dl protein is effectively blocked (see Figs. 2-4). However, labeling of cytosolic synthesized proteins continues during the chase as shown in Figs. 2-4. Addition of cycloheximide which could have prevented this further labeling was not used ...
Chapter 3 - HCC Learning Web
... • Carbohydrates include sugars and polymers of sugar. They include – small sugar molecules in energy drinks and – long starch molecules in spaghetti and French fries. ...
... • Carbohydrates include sugars and polymers of sugar. They include – small sugar molecules in energy drinks and – long starch molecules in spaghetti and French fries. ...
Protein (nutrient)
Proteins are essential nutrients for the human body. They are one of the building blocks of body tissue, and can also serve as a fuel source. As a fuel, proteins contain 4 kcal per gram, just like carbohydrates and unlike lipids, which contain 9 kcal per gram. The most important aspect and defining characteristic of protein from a nutritional standpoint is its amino acid composition.Proteins are polymer chains made of amino acids linked together by peptide bonds. During human digestion, proteins are broken down in the stomach to smaller polypeptide chains via hydrochloric acid and protease actions. This is crucial for the synthesis of the essential amino acids that cannot be biosynthesized by the body.There are nine essential amino acids which humans must obtain from their diet in order to prevent protein-energy malnutrition. They are phenylalanine, valine, threonine, tryptophan, methionine, leucine, isoleucine, lysine, and histidine. There are five dispensable amino acids which humans are able to synthesize in the body. These five are alanine, aspartic acid, asparagine, glutamic acid and serine. There are six conditionally essential amino acids whose synthesis can be limited under special pathophysiological conditions, such as prematurity in the infant or individuals in severe catabolic distress. These six are arginine, cysteine, glycine, glutamine, proline and tyrosine.Humans need the essential amino acids in certain ratios. Some protein sources contain amino acids in a more or less 'complete' sense. This has given rise to various ranking systems for protein sources, as described in the article.Animal sources of protein include meats, dairy products, fish and eggs. Vegan sources of protein include whole grains, pulses, legumes, soy, and nuts. Vegetarians and vegans can get enough essential amino acids by eating a variety of plant proteins. It is commonly believed that athletes should consume a higher-than-normal protein intake to maintain optimal physical performance.