![biochemistry module elective course contents](http://s1.studyres.com/store/data/001024944_1-6155202bed5807e76e1426ab112affd1-300x300.png)
Reprogramming the Genetic Code: From Triplet to Quadruplet Codes
... have been identified in E. coli and S. cerevisiae.[50–52] Moore and co-workers examined a limited range of extended anticodon tRNAs derived from E. coli tRNALeuCAA and discovered that E. coli tRNALeuUCUA efficiently decodes UAGA codons.[50] Magliery et al. set out to discover a set of functional ext ...
... have been identified in E. coli and S. cerevisiae.[50–52] Moore and co-workers examined a limited range of extended anticodon tRNAs derived from E. coli tRNALeuCAA and discovered that E. coli tRNALeuUCUA efficiently decodes UAGA codons.[50] Magliery et al. set out to discover a set of functional ext ...
AtCHIP functions as an E3 ubiquitin ligase of protein phosphatase
... 2003). Like animal CHIP proteins, AtCHIP has E3 ubiquitin ligase activity in vitro. AtCHIP transcripts were upregulated by several stress treatments including low and high temperatures. However, increased AtCHIP expression was not correlated with increased stress tolerance; in fact, overexpression o ...
... 2003). Like animal CHIP proteins, AtCHIP has E3 ubiquitin ligase activity in vitro. AtCHIP transcripts were upregulated by several stress treatments including low and high temperatures. However, increased AtCHIP expression was not correlated with increased stress tolerance; in fact, overexpression o ...
AFFINITY OF WARFARIN WITH CYP2C9 BY MOLECULAR DOCKING STUDY Original Article
... the most widely used oral anticoagulant drugs worldwide. They function as antagonists of vitamin K, an essential cofactor for the posttranslational γ-glutamyl carboxylation so called as vitamin Kdependent proteins. [3] The CYP2C9 ranks amongst the most important drug metabolizing enzymes in humans, ...
... the most widely used oral anticoagulant drugs worldwide. They function as antagonists of vitamin K, an essential cofactor for the posttranslational γ-glutamyl carboxylation so called as vitamin Kdependent proteins. [3] The CYP2C9 ranks amongst the most important drug metabolizing enzymes in humans, ...
Transport of Storage Proteins to Protein Storage Vacuoles Is
... proteins of pumpkin, namely, 11S globulin and 2S albumin, are not glycosylated. Recently, we isolated a cDNA of the third major storage protein of pumpkin, the 51-kD protein. The cDNA encodes the 100-kD precursor with no possible N-linked glycosylation site (data not shown). Thus, it is not necessar ...
... proteins of pumpkin, namely, 11S globulin and 2S albumin, are not glycosylated. Recently, we isolated a cDNA of the third major storage protein of pumpkin, the 51-kD protein. The cDNA encodes the 100-kD precursor with no possible N-linked glycosylation site (data not shown). Thus, it is not necessar ...
Characterization of a Novel Peripheral Nervous System Myelin
... MAG overexpression in mixed Schwann cell neuron cultures also provide support for the hypothesis that MAG is involved in axon-Schwann cell interactions (Owens et al., 1990) . Protein zero (PO), MBP, and MAG are the major protein components of PNS myelin (reviewed in Morell et al ., 1989; Lemke, 198 ...
... MAG overexpression in mixed Schwann cell neuron cultures also provide support for the hypothesis that MAG is involved in axon-Schwann cell interactions (Owens et al., 1990) . Protein zero (PO), MBP, and MAG are the major protein components of PNS myelin (reviewed in Morell et al ., 1989; Lemke, 198 ...
Leukaemia Section Anaplasic large cell lymphoma (ALCL) Atlas of Genetics and Cytogenetics
... various partners (see below); ALK+ ALCL cases are sometimes called ALK lymphomas, or ALKomas. ALK+ ALCL can be further divided into t(2;5) cases, with NPM1-ALK fusion protein which localises both in the cytoplasm and in the nucleus, and t(2;Var), involving various partners and ALK, and a cytoplasmic ...
... various partners (see below); ALK+ ALCL cases are sometimes called ALK lymphomas, or ALKomas. ALK+ ALCL can be further divided into t(2;5) cases, with NPM1-ALK fusion protein which localises both in the cytoplasm and in the nucleus, and t(2;Var), involving various partners and ALK, and a cytoplasmic ...
results
... (A) Five-week-old soil-grown ProNPR1:Myc-NPR1 aba3 plants were inoculated with Psm ES4326 (OD600 = 0.0001) with or without the indicated concentration of ABA. The in planta bacterial titers were determined three days postinoculation. Data represent the mean of eight independent samples with SD. Diff ...
... (A) Five-week-old soil-grown ProNPR1:Myc-NPR1 aba3 plants were inoculated with Psm ES4326 (OD600 = 0.0001) with or without the indicated concentration of ABA. The in planta bacterial titers were determined three days postinoculation. Data represent the mean of eight independent samples with SD. Diff ...
Methods for imaging and detecting modification of proteins
... prostane pathway [19]. Isoketals are highly electrophilic γ-ketoaldehydes that rapidly and covalently modify lysine residues, forming a variety of adducts including lactam, hydroxylactam, imine, and pyrrole species. The enzymatic oxidation of arachidonic acid also results in the formation of reactiv ...
... prostane pathway [19]. Isoketals are highly electrophilic γ-ketoaldehydes that rapidly and covalently modify lysine residues, forming a variety of adducts including lactam, hydroxylactam, imine, and pyrrole species. The enzymatic oxidation of arachidonic acid also results in the formation of reactiv ...
Dreze et al, Methods Enzymol 2010
... functional as truncated fragments and not when expressed in the context of full-length proteins (de novo autoactivators). Both genuine transcription factors and cognate DB-X autoactivators can be identified and removed by performing prescreens for reporter gene activation either with AD expressed al ...
... functional as truncated fragments and not when expressed in the context of full-length proteins (de novo autoactivators). Both genuine transcription factors and cognate DB-X autoactivators can be identified and removed by performing prescreens for reporter gene activation either with AD expressed al ...
Induction of reverse mutations with plate test in T26 and... Table 2.
... higher than that in the inl strain under similar conditions. With increasing inositol concentration, the antigen content becomes lower in each of the three strains. In the case of the wild type strain, even at an inositol concentration of 3 ug/ml and 6 ug/ml the inhibition is significant, i.e. 36% a ...
... higher than that in the inl strain under similar conditions. With increasing inositol concentration, the antigen content becomes lower in each of the three strains. In the case of the wild type strain, even at an inositol concentration of 3 ug/ml and 6 ug/ml the inhibition is significant, i.e. 36% a ...
Selective Dimerization of a C2H2 Zinc Finger Subfamily
... molecular weight of 67 kDa, consistent with the size of an IK I dimer (Figure 1D, lanes 19–21). A weak 85 kDa band was also observed, raising the possibility that IK I contains a domain that supports multimer formation, as previously suggested (Trinh et al., 2001). Crosslinked products were not obse ...
... molecular weight of 67 kDa, consistent with the size of an IK I dimer (Figure 1D, lanes 19–21). A weak 85 kDa band was also observed, raising the possibility that IK I contains a domain that supports multimer formation, as previously suggested (Trinh et al., 2001). Crosslinked products were not obse ...
intensity of strength training facts and theory
... scientific proof of the optimum results that can be expected from the employed training routines, reflects the most efficient training techniques known at the time. The distribution of training weights in the conditioning of elite weight lifters is shown in Figure 1. Elite athletes use a broad spect ...
... scientific proof of the optimum results that can be expected from the employed training routines, reflects the most efficient training techniques known at the time. The distribution of training weights in the conditioning of elite weight lifters is shown in Figure 1. Elite athletes use a broad spect ...
Keep Your Fingers Off My DNA: Protein–Protein
... However, the ZF-DNA contacts are virtually always mediated by amino acids located in the N-terminal portion of the a-helix. Unlike ZF-DNA interactions, the interacting residues responsible for specific ZF PPIs have been significantly less studied. In most cases, little is known about the binding sur ...
... However, the ZF-DNA contacts are virtually always mediated by amino acids located in the N-terminal portion of the a-helix. Unlike ZF-DNA interactions, the interacting residues responsible for specific ZF PPIs have been significantly less studied. In most cases, little is known about the binding sur ...
Domain organization of human cleavage factor Im 1 Distinct
... Eukaryotic messenger RNA precursors (pre-mRNAs) are synthesized and processed in the nucleus prior to their export to the cytoplasm, where they serve as templates for protein synthesis. Transcription is coupled spatially and temporally to capping of the premRNA at the 5’!end, splicing and 3’!end for ...
... Eukaryotic messenger RNA precursors (pre-mRNAs) are synthesized and processed in the nucleus prior to their export to the cytoplasm, where they serve as templates for protein synthesis. Transcription is coupled spatially and temporally to capping of the premRNA at the 5’!end, splicing and 3’!end for ...
Document
... 2045 ongoing projects, 348 completed, mostly from microbes (228 with more than 1500 genes, more or less correctly annotated) 144,116,054,623 nucleotides at International Nucleotide Sequence Database Collaboration (INSDC) Microbes make 50% of the Earth protoplasm 40-50% coding DNA sequences (CDSs) do ...
... 2045 ongoing projects, 348 completed, mostly from microbes (228 with more than 1500 genes, more or less correctly annotated) 144,116,054,623 nucleotides at International Nucleotide Sequence Database Collaboration (INSDC) Microbes make 50% of the Earth protoplasm 40-50% coding DNA sequences (CDSs) do ...
The subunit of voltage sensitive Ca 2+ channels is a single
... types of antibodies were used: one type was generated against a GST-~2-fusion protein (Leu 645 Tyr856; see section 2) and the other against a 19 amino acid peptide (LysSC~-lle526) within the alternatively spliced region [20-21]. As shown in Fig. 2B, the reduced a2/5 is expressed as a 140 kDa protein ...
... types of antibodies were used: one type was generated against a GST-~2-fusion protein (Leu 645 Tyr856; see section 2) and the other against a 19 amino acid peptide (LysSC~-lle526) within the alternatively spliced region [20-21]. As shown in Fig. 2B, the reduced a2/5 is expressed as a 140 kDa protein ...
Identification of a Second Collagen-Like
... discovered in B. anthracis (4), but a second collagen-like glycoprotein has not previously been identified in this organism. On electron microscopy observation of untreated spores, after ruthenium red staining, the exosporium is observed as a distinct carbohydrate-rich layer surrounding the underlyi ...
... discovered in B. anthracis (4), but a second collagen-like glycoprotein has not previously been identified in this organism. On electron microscopy observation of untreated spores, after ruthenium red staining, the exosporium is observed as a distinct carbohydrate-rich layer surrounding the underlyi ...
Cofactor requirement of ribosome-inactivating
... All the above-mentioned RIPs are type 1 RIPs, consisting of a single polypeptide chain of approximately 30 kDa, in contrast to type 2 RIPs, many of which are toxic, constituted of a catalytic polypeptide (A-chain) linked to a B-chain able to bind to cell surface receptors on target cells. Type 1 RIP ...
... All the above-mentioned RIPs are type 1 RIPs, consisting of a single polypeptide chain of approximately 30 kDa, in contrast to type 2 RIPs, many of which are toxic, constituted of a catalytic polypeptide (A-chain) linked to a B-chain able to bind to cell surface receptors on target cells. Type 1 RIP ...
Structural basis of ubiquitylation Andrew P VanDemark and
... salt bridges and hydrogen bonds in U-boxes. First identified as a domain within the Ufd2 protein, which is required for efficient polyubiquitylation of a model substrate [15], U-boxes have been shown to bind E2 enzymes and to autoubiquitylate when tested using the standard assay for RING E3 activity ...
... salt bridges and hydrogen bonds in U-boxes. First identified as a domain within the Ufd2 protein, which is required for efficient polyubiquitylation of a model substrate [15], U-boxes have been shown to bind E2 enzymes and to autoubiquitylate when tested using the standard assay for RING E3 activity ...
299279 Omaha Steaks and Burger package spec sheet
... Cholesterol Less than 300 mg Sodium Less than 2,400 mg 2,400 mg 300 g 375 g Total Carbohydrate 25 g 30 g Dietary Fiber Calories per gram: Fat 9 Ingredients: No Fillers or Additives ...
... Cholesterol Less than 300 mg Sodium Less than 2,400 mg 2,400 mg 300 g 375 g Total Carbohydrate 25 g 30 g Dietary Fiber Calories per gram: Fat 9 Ingredients: No Fillers or Additives ...
TOR SIGNALLING IN BUGS, BRAIN AND BRAWN
... Yeast cells grow and proliferate in response to ambient nutrients such as nitrogen and carbon sources. The quantity and quality of nutrient sources are detected by a diverse set of sensing and regulatory systems. In the presence of good nitrogen sources, such as ammonium or glutamine, the transcript ...
... Yeast cells grow and proliferate in response to ambient nutrients such as nitrogen and carbon sources. The quantity and quality of nutrient sources are detected by a diverse set of sensing and regulatory systems. In the presence of good nitrogen sources, such as ammonium or glutamine, the transcript ...
The Methylosome, a 20S Complex Containing JBP1 and pICln
... (then termed IBP72) (30), the results presented in Fig. 2 suggest that a JBP1-pICln complex and the SMN complex associate with different forms of the same Sm proteins. This suggested that these two complexes are separate and that the SMN complex binds the Sm proteins after they have been modified by ...
... (then termed IBP72) (30), the results presented in Fig. 2 suggest that a JBP1-pICln complex and the SMN complex associate with different forms of the same Sm proteins. This suggested that these two complexes are separate and that the SMN complex binds the Sm proteins after they have been modified by ...
Protein (nutrient)
Proteins are essential nutrients for the human body. They are one of the building blocks of body tissue, and can also serve as a fuel source. As a fuel, proteins contain 4 kcal per gram, just like carbohydrates and unlike lipids, which contain 9 kcal per gram. The most important aspect and defining characteristic of protein from a nutritional standpoint is its amino acid composition.Proteins are polymer chains made of amino acids linked together by peptide bonds. During human digestion, proteins are broken down in the stomach to smaller polypeptide chains via hydrochloric acid and protease actions. This is crucial for the synthesis of the essential amino acids that cannot be biosynthesized by the body.There are nine essential amino acids which humans must obtain from their diet in order to prevent protein-energy malnutrition. They are phenylalanine, valine, threonine, tryptophan, methionine, leucine, isoleucine, lysine, and histidine. There are five dispensable amino acids which humans are able to synthesize in the body. These five are alanine, aspartic acid, asparagine, glutamic acid and serine. There are six conditionally essential amino acids whose synthesis can be limited under special pathophysiological conditions, such as prematurity in the infant or individuals in severe catabolic distress. These six are arginine, cysteine, glycine, glutamine, proline and tyrosine.Humans need the essential amino acids in certain ratios. Some protein sources contain amino acids in a more or less 'complete' sense. This has given rise to various ranking systems for protein sources, as described in the article.Animal sources of protein include meats, dairy products, fish and eggs. Vegan sources of protein include whole grains, pulses, legumes, soy, and nuts. Vegetarians and vegans can get enough essential amino acids by eating a variety of plant proteins. It is commonly believed that athletes should consume a higher-than-normal protein intake to maintain optimal physical performance.