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Spring 2015-Chapter 8
Spring 2015-Chapter 8

... Genes are transferred in a linear sequence and the number of genes transferred depends on the duration of conjugation and whether the DNA strand breaks or remains intact. ...
Bill Nye: Genes
Bill Nye: Genes

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DNA Replication, RNA Molecules and Transcription
DNA Replication, RNA Molecules and Transcription

... A transcription reaction requires a DNA molecule to serve as template for transcription with a promoter (and, in vivo, transcription factors) to indicate where to begin transcribing and which strand to transcribe. Transcription reactions also require an RNA polymerase that recognizes the promoter on ...
Nerve activates contraction
Nerve activates contraction

... Different from eukaryotic chromosomes which have linear DNA molecules associated with large amounts of protein. Within bacterium, the chromosome is so tightly packed that it fills only part of the cell – dense region called nucleoid – NOT bound by membrane like the nucleus of eukaryotic cell. Replic ...
The Origin and Early Evolution of Life
The Origin and Early Evolution of Life

...  First cells (anaerobic bacteria) ...
Recombinant DNA technology article
Recombinant DNA technology article

... Recombinant DNA technology has applications in health and nutrition. In medicine, it is used to create pharmaceutical products such as human insulin. In agriculture, it is used to impart favorable characteristics to plant to increase their yield and improve nutritional content. Recombinant DNA techn ...
Genes - ASW Moodle
Genes - ASW Moodle

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Making RNA in other ways

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DNA gel electrophoresis

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Science 9: Unit A – Biological Diversity

... DNA, Proteins, and Genes • DNA forms chromosomes. Different sections of chromosomes act as blueprints for different proteins to be made. • A section of a chromosome that codes for a single protein is called a GENE. • A single chromosome can have hundreds or thousands of genes, made up of billions o ...
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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

... 1. a) The small fragments of DNA, produced during replication are called as --------------b) The enzyme catalyzing the synthesis of RNA from DNA is --------------------. 2. Give the structure of RNA polymerase. 3. Name the two antibiotics which act as ionophores for potassium (k+) ions. 4. What is t ...
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Lecture Notes with Key Images

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Mutations, Mutagenesis, and Repair

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AP genetic technology

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From Gene to Protein

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Unit Plan Template - Gates County Schools

... Bio.3.2.1 Explain the role of meiosis in sexual reproduction and genetic variation. Bio.3.2 Understand how the environment, and/or the interaction of alleles, influences the expression of genetic traits. Bio.3.2.2 Predict offspring ratios based on a variety of inheritance patterns (including: domina ...
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Palindromic Sequences

... separated through a process known as paper chromatography. A similar process was done for fraction A. From all the studies perform, the overall structure and cross linking between chains can be deduced. Cross-linking can occur on cysteine residues, because they contain sulfur atoms, capable of dimer ...
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Synoptic Activity 03_cards

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End of chapter 16 questions and answers from the text book
End of chapter 16 questions and answers from the text book

... 4. One technique used to determine the sequence of nucleotides in a sample of DNA is the Sanger procedure. This requires four sequencing reactions to be carried out at the same time. The sequencing reactions occur in four separate tubes. Each tube contains;  A large quantity of the sample DNA  A l ...
DNA & PROTEIN SYNTHESIS
DNA & PROTEIN SYNTHESIS

... found in DNA. b. Carries instructions to the ribosomes on how to make a specific protein. ...
Reading GuideBacterialViruses(Ch13)
Reading GuideBacterialViruses(Ch13)

... is integrated into a region that is similar, the new DNA material is “swapped” out for the old DNA). The area on the bacterial chromosome where lambda integrates is between the gal gene (codes for enzymes necessary for galactose metabolism) and the bio gene (codes for the synthesis of the vitamin bi ...
CHEM F450
CHEM F450

... 5. Once you have an account and are enrolled in the course, you can log in at any time to complete or review your homework assignments. 6. If you have problems, contact your instructor or send an email to [email protected]. Course Description: ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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