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Honors Genetics Chapter 2: Mitosis and Meiosis INTRODUCTION
Honors Genetics Chapter 2: Mitosis and Meiosis INTRODUCTION

... Chapter 2: Mitosis and Meiosis INTRODUCTION: Every living thing, except some viruses, contains DNA as the genetic material that houses the information to control the structure and function of the organism. A molecule of DNA is organized into units called genes, the products direct the metabolic acti ...
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doc - Genome: The Secret of How Life Works

... questions such as: “Where is the nucleus found?” “How big is a cell?” “What is a genome?” “What is a chromosome?” (Remember: A single human cell’s DNA stretched out is 2 meters long so some orders may cause a debate depending on what students know.) 6. When students have finished, discuss answers, a ...
Identify which nucleic acid (DNA or RNA) contains each of the
Identify which nucleic acid (DNA or RNA) contains each of the

... A mRNA has the sequence of codons 5′ CCC|AGA|GCC 3′ . If a base substitution in the DNA changes the mRNA codon of AGA to GGA, how is the amino acid sequence affected in the resulting protein? Can you predict whether this might have an effect on the protein function? ...
Biotechnology - Kinam Park Homepage
Biotechnology - Kinam Park Homepage

... Boyer’s Work with rDNA and Bacteria After Paul Berg’s 1971 landmark gene-splicing experiment, the next landmark in the development of modern biotechnology was the insertion of rDNA into bacteria in such a way that the foreign DNA would replicate naturally (see Figure). This step was taken in 1972 by ...
MUTATIONS • Mutations are errors made in the DNA sequence that
MUTATIONS • Mutations are errors made in the DNA sequence that

... Transposable Genes  discovered by Barbara McClintock  fragments of DNA that move from one location to another  may cause a disruption in transcription if they fall within a coding region Causes of Genetic Mutations  spontaneous mutations are caused by errors in the genetic machinery  Ex/ DNA Po ...
How are animal proteins made from DNA?
How are animal proteins made from DNA?

... • A part of the DNA double helix within the nucleus is ________, cut by _______, and then copied onto a new ______ ______, called mRNA. This process is called ___________.” • Once the DNA is transcribed, the single strand moves from the ______ to a ________ in the __________ of the cell. Thus the na ...
DNA switches
DNA switches

... least 80 percent of it is active and needed. The result is an annotated road map of much of this DNA, noting what it is doing and how. It includes the system of switches that, acting like dimmer switches for lights, control which genes are used in a cell and when they are used, and determine, for in ...
Voyager
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... nucleus of single cells. Some organisms like bacteria often have just one chromosome, while others like a fern have as many as 630. The genome refers to all of the DNA in a cell, no matter how many separate chromosomes there might be. ...
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Gene expression - El Camino College

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Protein Synthesis - Doral Academy High School
Protein Synthesis - Doral Academy High School

... RNA contains the base uracil (U) DNA has thymine (T) ...
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Analysis of Genomes

... e. found mutations in affected individuals in one gene f. wild-type gene rescued cells from affected individuals F. now, if clones available, get them; if genome sequenced, screen for candidates V. Functional genomics - determine function of all genes A. Mutation 1. identify all ORFs in sequence 2. ...
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Powerpoint slides - Berkeley Statistics

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Recombinant DNA technology File

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PS401-Mar. 17

... the protein that is important for function. Can help to ID the catalytic site or a site involved in protein-protein interactions or a site involved in transport, etc. ...
Gene Linkage
Gene Linkage

... 2. Cut the plasmid and the human DNA with the same restriction enzyme. 3. Use ligase to join the fragment of human DNA containing the insulin gene with the cut bacterial plasmid. 4. Insert the plasmid with recombinant DNA into a bacteria ...
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30. Insulin Prodution

... How did they make insulin from recombinant DNA? National Institutes of Health Recombinant DNA is a technology scientists developed that made it possible to insert a human gene into the genetic material of a common bacterium. This “recombinant” micro-organism could now produce the protein encoded by ...
1 Unit 9: Modern Genetics Advance Organizer Topic: DNA, RNA
1 Unit 9: Modern Genetics Advance Organizer Topic: DNA, RNA

... proteins called ________________ which allow DNA to coil in the nucleus. - __________________________________ first discovered the structure of DNA. - DNA looks like a _________-________ or twisted ladder under a microscope. - DNA is _____________ - _______________ and found in the ________________. ...
Griffith/Hershey/Chase
Griffith/Hershey/Chase

... Martha Chase in 1952 (figure 6.2). They chose to explore the genetic properties of DNA using bacterial viruses. Viruses are small, very simple aggregates of nucleic acid and protein. Several types of viruses attack bacteria and are known as bacteriophages (literally: “bacteria-eaters”). One of the v ...
hox genes - WordPress.com
hox genes - WordPress.com

... is junk DNA •PROMOTER REGIONS are associated with genes and help initialize transcription of the gene into a protein •GENETIC SWITCHES play a role regulating the EXPRESSION of genes ...
DNA
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... Each DNA strand has two ends; 5′ end ( the end with free phosphate group attached to C5′ of the first pentose) and 3′ end ( the end with free OH group on C3′ of the last pentose. The nucleotides in the polynucleotide chain is always read from 5′ → 3′ direction This part of polynucleotide is read AT ...
Lezione 25 - 26 mercoledì 11 maggio 2011
Lezione 25 - 26 mercoledì 11 maggio 2011

... simple to use, and flexible, but have the drawback of leaving recombination site sequences in the final construct, adding an extra 8 to 13 amino acids to the expressed protein. We have devised a simple and rapid subcloning strategy to transfer any DNA fragment of interest from an entry clone into an ...
04 Genetics of Bacteria and Viruses. Biotechnology
04 Genetics of Bacteria and Viruses. Biotechnology

... Complex transposons vary in length from about 2,000 to more than 40,000 nucleotide pairs and contain insertion sequences (or closely related sequences) at each end, usually as inverted repeats. The entire complex element can transpose as a unit. ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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