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File - The Science of Payne
File - The Science of Payne

Document
Document

... To identify the types of DNA sequences found within each class they must be cloned ...
HOW TO GET TO WHERE YOU WANT TO BE
HOW TO GET TO WHERE YOU WANT TO BE

... 
Our
programs
emphasize
prac@cal
laboratory
techniques
and
 
methods.

Star@ng
in
second
year
we
teach
how
to
grow,
characterize,
 
and

iden@fy

bacteria
and
extract
their
DNA.
In
our
third
and
fourth
year
 
we
have
intensive
courses
that
combine
molecular,
microbiological
 
and
biochemical
method ...
doc Feb 8th, 2010 notes
doc Feb 8th, 2010 notes

... string of DNA. 4. The recombinant DNA is then inserrted into a phage head in vitro, note thos phage does not replicate itself (the only viral DNA is the cos site). ...
Exam 2 - Faculty
Exam 2 - Faculty

... 4. Know general events in, and be able to recognize, the stages in mitosis: prophase, metaphase, anaphase, telophase/cytokinesis [note the names of the stages are covered in week 8’s genetics lab] 5. Why both cell division and controlled cell death (“apoptosis”) are required for development 6. Cance ...
BIG IDEA #2 - Science - Miami
BIG IDEA #2 - Science - Miami

...  Review major plant and animal cell organelle structure and function (i.e., cell wall, cell membrane, nucleus, cytoplasm, chloroplasts, mitochondria, and vacuoles)  Explain that every organism requires a set of instructions that specifies its traits and that genes located in chromosomes contain th ...
I. Biology (35 points total) The following questions cover some of the
I. Biology (35 points total) The following questions cover some of the

... DNA transfers information to mRNA in the form of a code defined by a sequence of nucleotides bases. During protein synthesis, ribosomes move along the mRNA molecule and "read" its sequence three nucleotides at a time (codon) from the 5' end to the 3' end. Each amino acid is specified by the mRNA's c ...
Vocabulary List
Vocabulary List

Glucose (sugar) and Oxygen
Glucose (sugar) and Oxygen

... 2. You would most likely be consuming amino acids if you ate which food item below? A. ...
Chapter 2
Chapter 2

... on earth and it never changed, this caused the Great Chain of Being to be made. Then the discovery of the new world led to people thinking that new kinds of animals existed because they saw lots of new variations and even unkown animals. John Ray came up with the concept of species and genus to help ...
Fact Sheet Describing Recombinant DNA and Elements
Fact Sheet Describing Recombinant DNA and Elements

... The following is an example of how adenovirus can be used in gene therapy to treat cystic fibrosis: Adenoviruses are good vectors for use in human gene therapy because it can infect cells in vivo, rather than manipulating the cells in vitro, and returning the cells to the body. In order to become a ...
Genomics and Behavior “Central Dogma” Outline
Genomics and Behavior “Central Dogma” Outline

... gene expression in tissue samples • Extremely sensitive, often referred to as quantitative PCR or qPCR • All methods depend on increasing fluorescent signal as target gene is amplified ...
Lecture
Lecture

... Exponentially through Multiple Thermal Cycles Original DNA target region ...
Nucleic acid
Nucleic acid

... The sugars and phosphates in nucleic acids are connected to each other in an alternating chain (sugar-phosphate backbone) through phosphodiester linkages. In conventional nomenclature, the carbons to which the phosphate groups attach are the 3'-end and the 5'end carbons of the sugar. This gives nucl ...
CHAPTER 10
CHAPTER 10

... – Gene duplication occurs within a portion of a single chromosome. – Duplication may occur by unequal crossing over between misaligned homologous chromosomes. – Duplication has played a major role in the evolution of multigene families. ...
Phase I: Computational Procedures: I. Measure original band
Phase I: Computational Procedures: I. Measure original band

... IV.    Obtain  the  DNA  sequence  for  this  region.   A.        There  were  two  different  ways  to  go  about  this:   1.    I  could  have  this  region  of  my  own  DNA  sequenced  by  an  external  lab,  but  this ...
Simulated Biodiversity Lab - ABC
Simulated Biodiversity Lab - ABC

Botana curus - ABC-MissAngelochsBiologyClass
Botana curus - ABC-MissAngelochsBiologyClass

... Test 7: Translating the DNA code to make a protein • Under each DNA sequence in your answer packet, write the complimentary messenger RNA base sequence. Note: Unlike during DNA replication, in the production of messenger RNA, the DNA base “A” specifies the RNA base “U”. • Write the complimentary se ...
9/20 Bacterial and viral genetics
9/20 Bacterial and viral genetics

... • Competent cells: cells that take up DNA • Transformants: cells that receive genetic material • Cotransformed: cells that are transformed by two or more genes ...
Gene Cloning
Gene Cloning

... main bacterial chromosome (non-integrative plasmid) The smaller plasmids make use of the host cell's own DNA replicative enzymes in order to make copies of themselves. The larger plasmids carry genes that code for special enzymes that are specific for plasmid replication. ...
EOC Benchmark Review!
EOC Benchmark Review!

... Pick teams, take a seat, and come up with a school appropriate team name for the ...
When DNA Changes – Chap. 17
When DNA Changes – Chap. 17

Protein Synthesis
Protein Synthesis

... • Messenger RNA (mRNA) copies DNA’s code & carries the genetic information to the ribosomes • Ribosomal RNA (rRNA), along with protein, makes up the ribosomes • Transfer RNA (tRNA) transfers amino acids to the ribosomes where proteins are synthesized ...
File
File

... "The genetic code is frequently referred to as a blueprint because it contains the instructions a cell requires in order to sustain itself. We now know that there is more to these instructions than simply the sequence of letters in the nucleotide code, however. For example, vast amounts of evidence ...
RT-PCR - Faperta UGM
RT-PCR - Faperta UGM

... Can identify variety genotypes and low levels of GM DNA Detection technique possible with GMOspecific primer and identifiable genomic primer ...
< 1 ... 481 482 483 484 485 486 487 488 489 ... 766 >

Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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