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outline File - selu moodle
outline File - selu moodle

... Begins at a promoter  transcribes the transcription unit  ends at the terminator Promoter – sequence within DNA Elongation uses RNA polymerase to add ribonucleotides that are complementary to the template strand Most common mechanism for termination is the formation of a hairpin structure In proka ...
Genetic Profiling using Short Tandem Repeat Analysis
Genetic Profiling using Short Tandem Repeat Analysis

... A  specific  STR  is  characterized  by  the  sequence  of  its  repeat  unit  and  the  number of times that unit is repeated.  Located on chromosome 5, the human c­  fms  proto­oncogene  for  CSF­1  receptor  gene  (CSF1PO),  is  an  example  of  a  tetranucleotide repeat polymorphism which has t ...
Unit Three “Cell Proliferation and Genetics”
Unit Three “Cell Proliferation and Genetics”

... Categories of RNA • Ribosomal RNA (rRNA) – DNA serves as template for production of rRNA; formed in the nucleus of a cell; moves into the cytoplasm to bond with proteins; rRNA + proteins make up Ribosomes (site of protein synthesis) • Messenger RNA (mRNA) – DNA serves as template for production of ...
Ch. 13.4: DNA Applications
Ch. 13.4: DNA Applications

... Genetic Markers ...
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D - What is electron transport?

... In some plants, when the soil pH is >7 blue flowers are produced and when the soil pH is <7 pink flowers are produced. It’s the best explanation for the color changes in these plants. A – Multiple alleles determine the color of the flowers. B – The change in flower color is the result of a mutation. ...
Cellular reproduction
Cellular reproduction

... – This process produces two daughter cells. Each daughter cell contains a complete set of chromosomes. ...
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... exposed nitrogen bases, according to the base-pairing rules Step 3 - Two DNA molecules that form are identical to the original DNA molecule ...
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Coding DNA

... controversy • Expression vectors contain the sequences necessary to express inserted DNA in a specific cell type • Transgenic organisms have had a foreign gene inserted in them. • Transgenic animals contain genes that have been inserted without the use of ...
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Evolution of DNA by celluLar automata HC Lee Department of

... • Uptake of alien DNA not detrimental • Alien DNA is random • Initial conspecific DNA is random as ...
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BT_Ch4_Presentation

... Gene – a section of DNA on a chromosome that contains the genetic code of a protein Nitrogenous base – an important component of nucleic acids (DNA and RNA), composed of one of two nitrogen-containing rings; forms the critical hydrogen bonds between opposing strands of a double helix Base pair – the ...
BASIC DNA
BASIC DNA

... – Regions of DNA which differ from person to person • Locus (plural = loci) – Site or location on a chromosome • Allele – Different variants which can exist at a locus • DNA Profile – The combination of alleles for an individual ...
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DNA

... Watson and Francis Crick: Determined the exact three dimensional structure of DNA as a double helix held together by H bonds. Won 1962 Nobel Prize.  DNA is an antiparallel double helix: 5’ end of one strand is paired to 3’ end of other strand.  A & T and G & C are paired up by hydrogen bonds  Two ...
Chapter 10: Molecular Biology of the Gene
Chapter 10: Molecular Biology of the Gene

... Watson and Francis Crick: Determined the exact three dimensional structure of DNA as a double helix held together by H bonds. Won 1962 Nobel Prize.  DNA is an antiparallel double helix: 5’ end of one strand is paired to 3’ end of other strand.  A & T and G & C are paired up by hydrogen bonds  Two ...
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Science - Mansfield ISD

... Why might redundancy in the genetic code be an advantage to a cell? ...
Name_______________________ Period___________ Chapter
Name_______________________ Period___________ Chapter

... Figure 20.4 in your text is a more detailed discussion of the gene cloning procedure shown in Figure 20.2. Explain the following key points. a. What is the source of the gene of interest? A particular species of hummingbird b. Explain why the plasmid is engineered with ampR and lacZ. AmpR is used be ...
Sequences vs Viruses: Producer vs Product, Cause and
Sequences vs Viruses: Producer vs Product, Cause and

... molecules could lead to specific traits and behaviors in the organism harbouring those molecules. After all, chemical analyses of DNA always showed the presence of the same four nucleotides and the structure of the DNA molecule appeared to be reasonably simple and uniform. In contrast, the organism ...
Nucleic Acids and Protein Synthesis
Nucleic Acids and Protein Synthesis

Chapter 20: Biotechnology - Staff Web Sites @ BBHCSD
Chapter 20: Biotechnology - Staff Web Sites @ BBHCSD

... Figure 20.4 in your text is a more detailed discussion of the gene cloning procedure shown in Figure 20.2. Explain the following key points. a. What is the source of the gene of interest? A particular species of hummingbird b. Explain why the plasmid is engineered with ampR and lacZ. AmpR is used be ...
From DNA To Protein
From DNA To Protein

... • Bases in DNA and bases in mRNA strands are same in all organisms • Only sequence of bases differs • Each codon of mRNA “codes” for a particular amino acid or a stop codon • Genetic code can be translated to determine amino acid sequence for proteins from any section of DNA • Since all organisms us ...
Leukaemia Section t(1;9)(p34;q34)  Atlas of Genetics and Cytogenetics
Leukaemia Section t(1;9)(p34;q34) Atlas of Genetics and Cytogenetics

... recombination. The SFPQ/NONO heterodimer enhances DNA strand break rejoining. SFPQ has homologous recombination and non-homologous end joining activities. SFPQ is associated with the RAD51 protein complex. Role in transcriptional regulation: SFPQ and PTK6 (protein tyrosine kinase 6, also called BRK) ...
mutation - UMDBIO101SUMMER2012
mutation - UMDBIO101SUMMER2012

... • Matthew Meselson and Franklin Stahl tested, in 1958, the three alternative hypotheses for the replication of DNA – they used radioactive isotopes of N to label DNA at different stages of replication – they found that DNA replication was semiconservative ...
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Pyrimidines

...  16s RNA is of manageable size and information content ...
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1 Evolution of Genome Size 1. The C

... Previously functional genes that have lost their function due to mutation (usually by a mutation that introduces a stop codon into the ORF or an insertion/deletion that disrupts the reading frame). In rare cases, genes may lose function due to parasitic or symbiotic relationship with their host. In ...
No Slide Title
No Slide Title

... holds sister chromatids together through metaphase INTERmolecular linking of two DNAs (compare to condensin) established at replication fork-preloaded in G1? degraded at onset of anaphase to allow sister separation cohesin in pericentromeric regions recruited by HP1/K9me, may be regulated differentl ...
practice exam 3_answer key
practice exam 3_answer key

... a. The 3 prime carbon is attached to a hydroxyl group b. The 3 prime carbon is attached to a phosphate group c. During DNA replication, neither strand grows from the 3 prime end d. The 3 prime end of one strand is next to (across from) the 3 prime end of the other strand e. None of the above are tru ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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