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Genetics Review Sheet
Genetics Review Sheet

... How are mitosis and meiosis different? Mitosis results in 2 diploid cells while meiosis results in 4 haploid. Meiosis undergoes 2 cell divisions, mitosis only has 1. Meiosis makes reproductive cells, mitosis makes body cells. PROTIEN SYNTHESIS Resources: Class notes, Flow Chart, practice notes from ...
Bioinformatics Research - Purdue University :: Computer Science
Bioinformatics Research - Purdue University :: Computer Science

Formation of Jello - University at Buffalo
Formation of Jello - University at Buffalo

... Formation of Jello ...
Chromosomes - TeacherWeb
Chromosomes - TeacherWeb

... Cell Division (Mitosis) 2 genetically identical daughter cells from 1 parent cell ¡  Before cells divide l  They duplicate their genetic material à ensures that each daughter cell receives an exact copy of the genetic material, DNA ¡  Makes ...
Week 4 Pre-Lecture Slides
Week 4 Pre-Lecture Slides

... Concept Questions ...
AP Biology – Molecular Genetics (Chapters 14-17)
AP Biology – Molecular Genetics (Chapters 14-17)

... or off followed by others in an orderly progression of events 3. Hierarchy of “gene control” exists in complex eukaryotes a. Homeotic genes ...
Chapter 12 Power point 2
Chapter 12 Power point 2

... the DNA code, and transcribe it into a different format so it can be translated into a protein. ...
5о end of mRNA 1 2 1 1 2 3 Protein Ribosome RNA
5о end of mRNA 1 2 1 1 2 3 Protein Ribosome RNA

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“Ancient” Viruses
“Ancient” Viruses

... protein-primed process that occurs in the nucleus: 1. A preterminal protein (pTP)/DNA polymerase (Pol) complex binds to the 3’ origin of replication using both E2 proteins. 2 dCTP is recruited to form a phosphodiester bond with the pTP serine. 3. Continuous 5’ to 3’ synthesis of DNA by viral polymer ...
Lecture 32 Slides
Lecture 32 Slides

... 5% of the human genome is found to be recently-duplicated large segments (>500bp, identity>95%). [JA Bailey, Science, 2002] The duplicated regions create mosaic structure. Some of the duplicated segments contain new genes. ...
Laboratory 9: Plasmid Isolation
Laboratory 9: Plasmid Isolation

... 1. growth of the bacterial culture, 2. harvesting and lysis of the bacteria, 3. purification of the plasmid DNA Growth of the Bacterial Culture Plasmids should be purified from bacterial cultures that have been inoculated with a single transformed colony picked from an agar plate. At all times, the ...
Slide 1 - Ommbid.com
Slide 1 - Ommbid.com

Making Recombinant DNA
Making Recombinant DNA

... recombinant vector molecules to find their way into individual bacterial cells. Bacterial cells are plated and allowed to grow into colonies. An individual transformed cell with a single recombinant vector will divide into a colony with millions of cells, all carrying the same recombinant vector. Th ...
Chapter 12 Molecular Genetics
Chapter 12 Molecular Genetics

... following base pairing rules, bond on the leading strand of DNA  Like DNA replication controlled by many enzymes Occurs in the nucleus ...
Wildlife Forensics Pre-Visit Lesson This pre
Wildlife Forensics Pre-Visit Lesson This pre

... A threadlike structure of nucleic acids and protein found in the nucleus of most living cells, carrying genetic information in the form of genes. ...
Biology B Final Review ANSWERS
Biology B Final Review ANSWERS

... In anaphase I homologous chromosomes go to opposite sides of the cell, in Anaphase II half of each chromosome go to the sides of each cell Devise a theory that explains why the most complex animals only reproduce sexually. They reproduce sexually because it provides more variation within the populat ...
Chromosomes in prokaryotes
Chromosomes in prokaryotes

... The DNA molecule may be circular or linear, and can be composed of 10,000 to 1,000,000,000 base pairs. Typically eukaryotic cells have large linear chromosomes and prokaryotic cells have smaller circular chromosomes. In eukaryotes, nuclear chromosomes are packaged by proteins into a condensed struct ...
Bacterial and Viral Genetic Systems
Bacterial and Viral Genetic Systems

Introduction to biotechnology
Introduction to biotechnology

... assessment is performed and adequate safety measures are used. Recombinant DNA technology or genetic engineering was first used to clone DNA segments in bacterial hosts in order to overexpress specific gene products for further studies. Recombinant DNA molecules have also been used to create GMOs su ...
-1- Biophysics 204 Graphics problem set - nucleic acid
-1- Biophysics 204 Graphics problem set - nucleic acid

... Describe two features of the GLI complex (such as orientation of fingers relative to DNA, spacing of fingers, structure of fingers, etc.) that are obviously similar to the Zif complex and two that are different. [Q8] What is finger 1 doing? The DNA to which fingers 2 and 3 are bound probably does no ...
Lec. 2 - DNA replication 1
Lec. 2 - DNA replication 1

... Then, Pol I degrades the RNA part with its 5’-3’ exonuclease activity, and replaces it with DNA. Pol I is not highly processive, so stops before going far. ...
problem set
problem set

... expression of the gene (Fig. 5.31). One common method by which expressed proteins are purified is via the attachment of an amino acid sequence such as a polyhistidine sequence (Histag) that serves as a tag for affinity purification. Mammalian cell expression systems offer the advantage that posttran ...
Mutated - Olympic High School
Mutated - Olympic High School

... (think about it: are there 3 million differences between 2 people?) ...
MB207Jan2010
MB207Jan2010

... chromosomes during meiosis • Chromosome must synapse (pair) in order for chiasmata to form where crossing-over occurs – DNA synapsis: base pairing between complementary strands from 2 DNA molecules – Chiasmata: regions where paired homologous chromosomes exchange genetic material during meiosis, a c ...
3687317_mlbio10_Ch13_TestA_3rd.indd
3687317_mlbio10_Ch13_TestA_3rd.indd

... 2. Which nucleotide in Figure 13–1 indicates the nucleic acid above is RNA? a. uracil c. cytosine b. guanine d. adenine 3. What is produced during transcription? a. RNA molecules c. RNA polymerase b. DNA molecules d. proteins 4. During eukaryotic transcription, the molecule that is formed is a. comp ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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