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Chapter 25: Molecular Basis of Inheritance
Chapter 25: Molecular Basis of Inheritance

... a double helix in which A is paired with T and G is paired with C. This is called complementary base pairing because a purine (2 rings) is always paired with a pyrimidine (1 ring). ...
Plasmid
Plasmid

... BIOTECHNOLOGY ...
Meiotic DSBs and the control of mammalian recombination
Meiotic DSBs and the control of mammalian recombination

... well reflect the relative age of each allele. Boulton et al. [14] pointed out a number of years ago that because the chromatid on which the initiating DSB occurs is repaired using its intact partner as a template, mutations within hotspots that reduce their activity should be selected over time unti ...
DNA
DNA

... 4. The tRNA is recycled to find another of the same amino acid so the process can occur again and again. 5. The protein chains are then transported to other areas of the body that need them ...
Portfolio 4 Index
Portfolio 4 Index

... 26- Observe The table on pg. 414 in your book, shows the DNA sequences that are recognized by five different restriction enzymes and the location where those enzymes cut. Which enzymes produce DNA fragments with “sticky ends”? What is the common feature of the sequences cut by these enzymes? ...
VNTR, STR and RFLP
VNTR, STR and RFLP

slg mock midterm – for practice only
slg mock midterm – for practice only

... b. Each strand of both daughter molecules contains a mixture of old and newly synthesized DNA. c. The two strands of the parental molecule separate, and each functions as a template for synthesis of a new, complementary strand. d. DNA Polymerase III carries out synthesis by extending from the RNA pr ...
Unit I
Unit I

... dimensional shapes. If we could see a typical protein, it might look like this: ...
Document
Document

... A) DNA from the live R-strain was taken up by the heat-killed S-strain, converting them to R-strain and killing the mouse. B) DNA from the heat-killed S-strain was taken up by the live R-strain, converting them to S-strain and killing the mouse. C) Proteins released from the heat-killed S-strain kil ...
Luther Burbank produced over 800 varieties of plants by
Luther Burbank produced over 800 varieties of plants by

... Inbreeding can produce an offspring that has a defect that neither parent shows. Hybridization is used to produce new varieties of plants and animals. ...
b. genetic engineering.
b. genetic engineering.

... • A. Cloning Vector- a carrier that is used to clone a gene and transfer it from one organism to another. • B. Donor gene- specific gene from another organism spliced into a plasmid, that replicates as the bacteria divide – 1. A plasmid is a circular DNA molecule found in bacteria. C. Gene Clone- e ...
Powerpoint
Powerpoint

... Hardy-Weinberg equilibrium applies to portions of the genome that do not affect phenotype ...
amino acid
amino acid

... Steps of DNA Replication 1. Double stranded DNA is cleaved into two separate strands by the enzyme DNA helicase. DNA helicase “unzips” the DNA by breaking the bonds between the nitrogen base pairs. 2. Next, free floating nucleotides are matched to their complementary nucleotides through base pairin ...
The Human Genome Project
The Human Genome Project

... region of the genome, produce a virtually unlimited number of copies of it, and determine its nucleotide sequence overnight. • At the height of the Human Genome Project, sequencing factories were generating DNA sequences at a rate of 1000 nucleotides per second 24/7. • Technical breakthroughs that a ...
Genomics
Genomics

... Proteins are responsible for an endless number of tasks within the cell. The complete set of proteins in a cell can be referred to as its proteome and the study of protein structure and function and what every protein in the cell is doing is known as proteomics. The proteome is highly dynamic and it ...
DNA Quantification
DNA Quantification

... Checking the quality by agarose gel electrophoresis Genomic DNA extraction reading at OD260 is equivalent to 50 µg/ml). A pure DNA solution has anOD260:OD280 ratio of 1.8 ± 1.The DNA concentration is calculated using the formula, DNA concentration (µg /µl) = OD at 260 nm × dilution times × standard ...
LANDMARKS OF MODERN BIOLOGY
LANDMARKS OF MODERN BIOLOGY

... 1953 - After examining Franklin's unpublished data, James D. Watson and Francis Crick publish a double-helix structure for DNA. 1953 - Eugene P. Odum publishes the first textbook in the field: Fundamentals of Ecology and puts environmental studies on a scientific basis. 1955 - Marianne Grunberg-Mana ...
Chapter_9_Student
Chapter_9_Student

... Transforming - Bacteria During transformation, a cell takes in DNA from outside the cell. The external DNA becomes a component of the cell's DNA. Foreign DNA is first joined to a small, circular DNA molecule known as a plasmid. ...
Making Transgenic Plants and Animals
Making Transgenic Plants and Animals

... bioengineered drugs (e.g., use plants instead of animals or bacteria). ...
DNA PPT
DNA PPT

... be produced ...
DNA –Protein synthesis
DNA –Protein synthesis

... Where does transcription occur? ...
Mycoplasma genitalium
Mycoplasma genitalium

... – CVS or amniocentesis – Gene obtained from fetal DNA • Sickle cell anemia caused by a single nucleotide base substitution that removes a MstII site. – Different banding pattern on gel indicates whether fetus will be a carrier or have disease (homozygous) ...
bio12_sm_07_2
bio12_sm_07_2

... 6. DNA Replication and Transcription DNA replication Both DNA transcription - produces 2 semi-create new -produces a conserved double complementary nucleic single strand of stranded DNA molecules acid strands mRNA -uses DNA polymerase -read DNA code -use RNA polymerase 7. Answers may vary. Sample an ...
Zoo/Bot 3333
Zoo/Bot 3333

... Samples of DNA obtained from a fetus (F) and her parents (M and P) were cut by restriction enzyme R, then analyzed by gel electrophoresis followed by the Southern blot technique and hybridization with the radioactively labeled DNA probe designated “CF probe” in the above figure. Enzyme R has a six b ...
here - Quia
here - Quia

... 10. Given a DNA template, know how to transcribe and translate it. 11. Distinguish between prokaryotic and eukaryotic DNA. 12. Discuss the different types of mutations and their effect on protein synthesis. 13. Identify the location where protein synthesis in a eukaryotic cell. 14. List and explain ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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