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bioinformatics_project
bioinformatics_project

Microbes in nutrition Digestion vast majority of GI tract bacteria are
Microbes in nutrition Digestion vast majority of GI tract bacteria are

... not known to occur abiotically) d. certain hydrocarbons are optically active (known to occur only as a result of biological systems) e. data not conclusive - all features could have resulted from microbial activity after oil was formed C. Applied microbiology 1. the properties of specific microorgan ...
MCA Test Prep Answers Part 1
MCA Test Prep Answers Part 1

... “Computers because the scientist can graph the data and make models for better understanding.” Gel Electrophoresis, Polymerase Chain Reactions, Micropipettes, Di-deoxy-nucleotides, Stem Cells, etc. 2/2: Student correctly names two advances in scientific technology that have led to an increased under ...
Arabidopsis is Cool
Arabidopsis is Cool

... June 27, 2008 ...
1 DNA PHENOTYPING: PREDICTING ANCESTRY AND PHYSICAL
1 DNA PHENOTYPING: PREDICTING ANCESTRY AND PHYSICAL

... approaches for ancestry inference, principal component analysis and statistical clustering, both of which are performed at global and regional scales. Both require a database of reference DNA samples with well-defined ancestry, and thousands of subjects have been collected from populations around th ...
New techniques for genetic modification of plants
New techniques for genetic modification of plants

... genes with their introns and flanked by their ‘native’ promoter and terminator regions in the normal ‘sense’ orientation, whereas ‘intragenes’ refers to genes with interchanged ‘native’ endogenous regulatory regions to modify the desired expression of the endogenous gene. The terms ‘cisgenic’ or ‘ci ...
Chapter 12
Chapter 12

... 12.4 Enzymes are used to “cut and paste” DNA • Restriction enzymes cut DNA at specific points • DNA ligase “pastes” the DNA fragments together ...
University of Groningen Ion induced radiation damage on the
University of Groningen Ion induced radiation damage on the

UNIT ONE Exam Review 2013 - Mr. Lesiuk
UNIT ONE Exam Review 2013 - Mr. Lesiuk

... 21. This string like substance bunches up to form X-shaped structures just before cell division. What are these X-shaped structures called? 22. How many of these X-shaped structures are found in a human diploid cell, how about in a haploid human cell? 23. Describe what a gene is? 24. Compare and con ...
Electronic supplementary material
Electronic supplementary material

... hEDF1 than in the 434 repressor. This observation is consistent with the fact that the third helix contains more hydrophobic residues in Ctd-TrMBF1 and hEDF1 than in 434 repressor, especially at their N-termini (helix III residues are AATVASY, PQVIADY and QQSIEQL for Ctd-TrMBF1, hEDF1 and 434 repres ...
Direct measurement of electrical transport through DNA molecules
Direct measurement of electrical transport through DNA molecules

... states7,8, which could, for example, be associated with the base pairs. The hopping process could be either unidirectional or involve one-dimensional diffusion. It can be argued that the back-and-forth diffusive hopping8 is less likely in our case due to the high electric ®elds used, which will tilt ...
$doc.title

... • Strong  physical,  chemical  and  biological  heterogeneity  of  natural  environments.   • Huge  natural  microbial  diversity  and  strong  compe]]on.     • No   natural   “superbugs”.   Bacteria   more   or   less   adapted   to   specific   ...
No Slide Title
No Slide Title

... Naturally Extra chromosomal circular DNAs ...
MOLECULAR GENETICS You Are Here* Genes --
MOLECULAR GENETICS You Are Here* Genes --

... would encourage introns to proliferate, mutate, evolve ...
Author - Princeton ISD
Author - Princeton ISD

... Some students may think that the purpose of protein synthesis is to make amino acids. Since they work hand in hand, students are always hearing the terms used together. As a result, students often lose track of where amino acids originate from, and the purpose of protein synthesis. Once synthesized ...
Protocols - BioMed Central
Protocols - BioMed Central

... d. Heat shock the cells by incubating them at 42 oC for 45 seconds, immediately afterward return the cells to ice and incubate for 2 minutes. e. Add 450 l SOC medium, mix by inverting the tubes a couple of times and incubate for 1 hour at 37 oC with 300 rpm. f. Pellet the cells in a table top centr ...
Cellular Gate Technology - MIT Computer Science and Artificial
Cellular Gate Technology - MIT Computer Science and Artificial

... types. Each of the approximately 500-10,000 protein types in a typical cell consists of a unique sequence of the 20 amino acids. Moreover, each protein chain folds into a characteristic three-dimensional structure, which is necessary for its activity. Many proteins, called enzymes, act as exquisitel ...
Genes
Genes

...  synthesizing end products ...
Vectors
Vectors

... DNA into many copies. A DNA fragment must be inserted into a cloning vector. A cloning vector is a DNA molecule that has an origin of replication and is capable of replicating in a bacterial cell. ...
DNA
DNA

... - a double helix of two nucleotide strands linked together by pairs of organic bases which are joined together by hydrogen bonds - C pairs with G by 3 hydrogen bonds; A pairs with T by 2 hydrogen bonds; consistent with the known ratio of bases in molecule and allowed for an identical separation of s ...
Assignment - San Diego Mesa College
Assignment - San Diego Mesa College

... b. Write down the genotypes for the depicted family members at the bottom of the shown RFLP blot box. (Use the lower case “m” letter for the mutated version of the MRE gene and the capitalized “M” letter for the normal MRE gene!) c. Use the information supplied by the MRE gene map (= shown restricti ...
You Are What You Eat
You Are What You Eat

... Action ...
Serge Ankri - WordPress.com
Serge Ankri - WordPress.com

... As opposed to aminoacyl-tRNA synthetase, examples of modification enzymes that recognize only a single out of all 40-odd tRNA species are extremely rare, and for methyltransferases such cases are not known. This raises the question of the substrate specificity of Ehmeth and the ability of Ehmeth to ...
What is Biology? The word biology is 1………………………. from the
What is Biology? The word biology is 1………………………. from the

... sequences of DNA known as 7……………………………. regions. These regions may be thousands of base pairs distant from the start of the gene. Contact between the activator proteins and the initiationcomplex releases the copying mechanism. The RNA polymerase 8……………………………. a small portion of the DNA helix exposin ...
Slide 1 - The Fluorescence Foundation
Slide 1 - The Fluorescence Foundation

... Novel fluorescent proteins are incorporated into many of the our popular vectors, designed for: constitutive fusion protein expression in mammalian cells, subcellular localization of organelles or targeting of fusion proteins to a specific location, transcriptional reporting bacterial expression and ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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