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DNA webquest
DNA webquest

... http://www.sps186.org/teachers/stash/?b=52 home page gives you the link to this assignment in electronic form so that you can directly click on the links and go straight to the websites. Otherwise, you can just type the link into your browser. ...
Myriad and Prometheus
Myriad and Prometheus

... Inquiry 1: The claim is a process claim. Inquiry 2: The claim includes the limitation of the correlation between rheumatoid arthritis and the rheumatoid factor IgM, which is a natural principle/law of nature. Inquiry 3: The additional step of using a particular anti-IgM antibody (especially one that ...
Lac Operon
Lac Operon

... Families of DNA-binding domains (A) Zinc finger domains consist of loops in which an α helix and a β sheet coordinately bind a zinc ion. (B) Helix-turn-helix domains consist of three (or in some cases four) helical regions. One helix (helix 3) makes most of the contacts with DNA, while helices 1 an ...
Bio EOC Review Packet: Answers
Bio EOC Review Packet: Answers

... Mendel’s Laws/Genetics (SC.912.L.16.1) 68. Explain Mendel’s Law of Segregation & Law of Independent Assortment Law of Segregation- during the production of gametes, the factors (gene/allele) separate so that the offspring acquires one factor from each parent Law of Independent Assortment- Factors f ...
Chapter 6
Chapter 6

... Stanley Cohen and Herbert Boyer were familiar with the botanical use of the term chimera when they began the collaboration that would result in their chimeric DNA molecules, ones in which DNA from two different species were spliced together. The technique they developed—at about the same time as Dow ...
Internet Project – HUMAN GENOME PROJECT
Internet Project – HUMAN GENOME PROJECT

Center for Eukaryotic Structural Genomics (CESG)
Center for Eukaryotic Structural Genomics (CESG)

... We have made significant progress toward implementing a clone-free translation protocol for screening new protein constructs. The protocol relies on overlap extension PCR to fuse two PCR products together. One of these products contains the SP6 promoter, the TMV omega translational enhancer, and the ...
Biology EOC Review Pack
Biology EOC Review Pack

... 89) What problems could be associated with genetically modified organisms? 90) What is the Human Genome Project? Why is it useful? 3.05 Examine the theory of evolution by natural selection. 91) Contrast abiogenesis (spontaneous generation) and biogenesis. 92) What did Louis Pasteur contribute to our ...
PDF of the article
PDF of the article

... What does the Roadmap Epigenomics Initiative actually investigate? What is the goal of the project? • As the name of the project suggests, the idea is to draw up a map – to gain a survey – of the modifications at hand and their contribution to genetic regulation. We aim to identify the differences b ...
Sequencing
Sequencing

Human Genome
Human Genome

... • #21 - 32 million DNA base pairs, 225 genes, responsible for ALS (Lou Gehrig’s disease). • #22 – 43 million DNA base pairs, 545 genes, responsible for many genetic diseases, Leukemia, and tumor causing diseases of the nervous system. • Both have spaces on the chromosome that do not code for protein ...
ie inbred strains
ie inbred strains

... used to signify any gene orthologs, for example in human. A gene symbol must i) be unique, ii) be short (normally 3-5 characters), iii) begin with an uppercase letter (not a number), followed by all lowercase letters / numbers, iv) be italicized, v) comprise only Roman letters and Arabic ...
Restriction Enzymes and Electrophoresis - Milton
Restriction Enzymes and Electrophoresis - Milton

... identical on both strands (the 5’ and 3’ ends refers to the chemical structure of the DNA). Each of the double strands of the DNA molecule is complimentary to the other; thus adenine pairs with thymine, and guanine with cytosine. Restriction enzymes (also known as restriction endonucleases) recogniz ...
Cellular Control revision - Mrs Jones A
Cellular Control revision - Mrs Jones A

Review Materials for Chapter 14-16
Review Materials for Chapter 14-16

... You are handed a “mystery’’ pea plant with long stems and axial flowers, and asked to determine its genotype as quickly as possible. You know the allele for long stems (L) is dominant to that for dwarf stems (l) and that the allele for axial flowers (A) is dominant to that for terminal flowers (a). ...
Greed Exam 4
Greed Exam 4

... A: (the first life forms contained RNA instead of DNA as the genetic material) ...
STUDY GUIDE EXAM I
STUDY GUIDE EXAM I

... 5. How does DNA replication occur? What are the enzymes involved? What is the difference between a leading and a lagging strand? What does it mean that DNA replication is semi conservative? 6. What is transcription? What are the enzymes called? Where does it start on the DNA strand? Where does it oc ...
Genetics 200A Monday, September 28, 2009 Day 5: Yeast Lecture
Genetics 200A Monday, September 28, 2009 Day 5: Yeast Lecture

... 2x ARS: Origins of replication (1x ARS for circular plasmid) ***CEN-ARS plasmids are self-replicated in yeast*** Replicated DNA (“sister chromatids”) held together by cohesin S. cerevisiae Mitosis (M) Metaphase Each chromosome captures a single microtuble (from each centrosome) in yeast Centrosome i ...
Protein Synthesis Role Modeling Activity
Protein Synthesis Role Modeling Activity

... former Soviet Union. Most people don’t realize that mutations are any change to the DNA sequence. The genetic disorders that you have been researching are usually the result of one or two nucleotides in DNA that have been changed, added, or removed. Since DNA codes for protein, these DNA changes may ...
Mutations Worksheet
Mutations Worksheet

... FRAMESHIFT, meaning the reading “frame” changes, changing the amino acid sequence. DELETION (a base is lost) INSERTION (an extra base is inserted) SUBSTITUTION (one base is substituted for another) If a substitution changes the amino acid, it’s called a MISSENSE point mutation. If a substitution doe ...
Procedure - DNA Interactive
Procedure - DNA Interactive

... DNA elements, popularly known as "jumping genes", would offer an explanation to gene expression patterns in plants that Mendel's Laws failed to provide. It took the development of recombinant DNA methods and the discovery of transposable elements in everything from bacteria to humans, to give her id ...
Biology 10.2 Review Genes to Proteins
Biology 10.2 Review Genes to Proteins

... the AUG codon on the same mRNA and begin making a second copy of the same protein. •In this way many copies of the same protein are made from a single mRNA molecule. •With few exceptions, the genetic code is the same in all organisms. For this reason, the genetic code is often described as being nea ...
Document
Document

... Genome–wide Association Studies = GWAS ...
Biology 10.2 Review Genes to Proteins
Biology 10.2 Review Genes to Proteins

... the AUG codon on the same mRNA and begin making a second copy of the same protein. •In this way many copies of the same protein are made from a single mRNA molecule. •With few exceptions, the genetic code is the same in all organisms. For this reason, the genetic code is often described as being nea ...
CP Final Exam Study Guide 2015KEY
CP Final Exam Study Guide 2015KEY

... alleles a person receives.  Explain the difference between homozygous and heterozygous Homozygous describes an individual that has two of the same alleles for a given trait; heterozygous describes an individual who has two different alleles for a given trait.  Define heredity: The passing on of tr ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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