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Chapter 20~ DNA Technology & Genomics
Chapter 20~ DNA Technology & Genomics

... DNA ligase joins the strands sticky ends stick together ...
A new method of finding similarity regions in DNA sequences
A new method of finding similarity regions in DNA sequences

... Parameters used to group seeds are estimated according to probability distributions, assuming a Bernoulli model of DNA sequence. Three probability criteria have been used: • the seed size and the minimal seed number which triggers grouping, are computed from the minimum repeat size and the minimal s ...
Prokaryotes - The Bio Edge
Prokaryotes - The Bio Edge

... 45. Streptococcus sanguis is one of the bacteria that can cause the loss of tooth enamel in humans. The metabolic process that this particular species of bacteria uses that can result in enamel breakdown is A. Streptococcus sanguis, which can ferment sugar into lactic acid, which increases the pH of ...
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... human and chimpanzee, we aligned 14.3 Mb of orthologous noncoding DNA sequences from human, chimpanzee, and baboon (Methods). Subsequently, human chromosomes were split into 1 Mb non-overlapping windows (referred to as loci). We retained 36 loci dispersed over 12 autosomal chromosomes, each of which ...
MUTATIONS - Valhalla High School
MUTATIONS - Valhalla High School

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CH. 12.3 : DNA, RNA, and Protein
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standard set 5 - EDHSGreenSea.net

... 3. Since cells have to pass an exact copy of their hereditary instructions from one generation to the next DNA must be faithfully copied before cells divide. The structure of DNA (in Watson & Crick famous understatement) "…suggests a possible copying mechanism for the genetic material." DNA is the " ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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