• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Mutation and Genetic Variation - Cal State LA
Mutation and Genetic Variation - Cal State LA

... This story is important because it shows that you can challenge the basic theory of evolution through scientific experimentation – but careful experiments done for the last 150 years have, so far, all supported the premise of Darwinian evolution by selection ...
DNA Testing Info
DNA Testing Info

... directly from an easy-to-take sample. DNA testing may one day allow selection for carcass traits, such as muscling and tenderness, feed efficiency, out of season breeding and disease and parasite resistance. Already today, two disorders and diseases that in the past two decades have been detrimental ...
File - western undergrad. by the students, for the students.
File - western undergrad. by the students, for the students.

... The lac operon contains the genes required for the metabolism of lactose in E.coli. Studies of its expression have been integral to our understanding of gene expression. Jacob and Monod won a Nobel Prize for their pioneering work on the lac operon. Expression of lac is controlled by two signals. The ...
File
File

... Each strand of the DNA double helix has all the information needed to reconstruct the other half by the mechanism of base pairing. In most prokaryotes, DNA replication begins at a single point and continues in two directions. ...
Objective 2.1 Lesson D Recombinant Organisms
Objective 2.1 Lesson D Recombinant Organisms

... 6. How does the calcium chloride method solve the problem mentioned in the previous question? How does temperature change complement the use of calcium chloride? 7. __________ became the workhorses of the public Human Genome Project. 8. Name the five different vectors that are used as “DNA libraries ...
Lecture Notes
Lecture Notes

... decoding RNA molecules, by enzymes, by energy, all in a macromolecular assembly complex. ...
Wide Hybridization in Plant Breeding
Wide Hybridization in Plant Breeding

... Courtesy of Adam J. Lukaszewski, UCR ...
chapter 1 introduction
chapter 1 introduction

... mapping (Botstein et al., 1980), and later adopted for plant genomes (Helentjaris et al., 1986; Weber and Helentjaris, 1989). The technique is based on restriction enzymes that reveal a pattern difference between DNA fragment sizes in individual organisms. Although two individuals of the same specie ...
6. DNA transcription/translation
6. DNA transcription/translation

... E. coli and more than 130 repair enzymes identified in humans. A hereditary defect in one of these enzymes is associated with a form of colon cancer. ...
Recombination Frequency - Westford Academy Ap Bio
Recombination Frequency - Westford Academy Ap Bio

Meyer Prometheus Presentation - American Intellectual Property
Meyer Prometheus Presentation - American Intellectual Property

... These materials are public information and have been prepared solely for educational and entertainment purposes to contribute to the understanding of U.S. intellectual property law and practice. These materials reflect only the personal views of the speaker and are not individualized legal advice. I ...
Chapter 1. Introduction
Chapter 1. Introduction

... Subsequently, it has been demonstrated that genes reside on chromosomes, and chromosomes are passed from one generation to the next. ...
Epigenase™ 5-mC Hydroxylase TET Activity/Inhibition
Epigenase™ 5-mC Hydroxylase TET Activity/Inhibition

... The EpiQuik™ Methyl-CpG Binding Domain Protein 2 ChIP Kit contains all reagents required for carrying out a successful chromatin immunoprecipitation for MBD2 from mammalian cells. Particularly, this kit includes a ChIP-grade MBD2 antibody and a negative control normal mouse IgG. Chromatin from the c ...
Course Competencies Template
Course Competencies Template

... Explaining linked genes and solving linked genes problems using the results of linked gene crosses to determine the map distance of the genes on the chromosome. ...
Lisa Byers UNIT 6: Genetic Transformations Unit Plan
Lisa Byers UNIT 6: Genetic Transformations Unit Plan

... - What would you expect your experimental results to indicate about the effect of ampicillin on the E. coli cells? - What other process have we just learned about that we know can utilize gene therapy? - What would the gene code for? - On which of the plates would you expect to find bacteria most li ...
A Sex Chromosome Rearrangement in a Human XX
A Sex Chromosome Rearrangement in a Human XX

... the paternal Y but also a proximal portion of the pseudoautosomal region of the paternal X. In this XX male, the recombination point on the X is likely to lie within the pseudoautosomal region (Page et al., 1987a). Taken together, these studies show that in Y(+)XX males the positions of both the Yp ...
some recent developments in genetics
some recent developments in genetics

... like the hemoglobin of red blood cells, to important structural proteins like collagen. In addition, some genes appear to code for specific proteins, called repressors, which can bind to specific sites on DNA, and thus block the transcription of RNA messages from other specific genes.3 Thus, the pro ...
TNA: Transcription and Triplet Code
TNA: Transcription and Triplet Code

... Bases 1-7 are paired with bases 66-72 to form a double stranded (DS) region in the tRNA that makes it stable/stronger. This region extends through bases 73-76. The whole "arm" is known as the acceptor stem. Note that the 3' -OH is the site of attachment of the amino acid under the direction/catalysi ...
Genetic Markers and linkage mapping - genomics-lab
Genetic Markers and linkage mapping - genomics-lab

... nucleases. As shown, the target sites at which these enzymes cut have a nucleotide sequence and length that depend on the enzyme. Target sequences are often palindromic (that is, the nucleotide sequence is symmetrical around a central point). In these examples, both strands of DNA are cut at specifi ...
Prof. Kamakaka`s Lecture 14 Notes
Prof. Kamakaka`s Lecture 14 Notes

... In the region of the human X chromosome, two forms of the X-chromosome are Segregating in the population. ...
Environmental DNA in rivers can assess broad
Environmental DNA in rivers can assess broad

Chapter 3
Chapter 3

... eukaryotes so majority of the library will contain non-coding pieces of DNA • Many organisms have very large genome, so searching for gene of interest is difficult at best ...
Agrobacterium
Agrobacterium

Suracell: My Test Results
Suracell: My Test Results

... How do we know the efficiency of your genes in each category? Our DNA contains approximately 30,000 genes. Within our cells, our DNA is organized into 23 pairs of chromosomes. Genetic variations in our DNA are called SNPs (Single Nucleotide Polymorphisms). If we compare the DNA of two individuals, w ...
[Ni(II)(salen)] complex.
[Ni(II)(salen)] complex.

... Around the world, cancer is one of the leading causes of death. DNA is the primary target molecule for most anticancer therapy. Transition metal complexes are known to have DNA binding and cleavage properties under physiological conditions. Currently, we are investigating the interactions between a ...
< 1 ... 278 279 280 281 282 283 284 285 286 ... 766 >

Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report