• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Location of Exons in DNA Sequences Using Digital Filters
Location of Exons in DNA Sequences Using Digital Filters

... The complete set of instructions to build and maintain a living organism is encoded in its genome. The genome is made of DNA which is a biomolecule composed of smaller components called nucleotides [1]. A nucleotide can be one of four possible types, namely, adenine, thymine, guanine, and cytosine d ...
Exam 2 Full v3 Bio200 Win16
Exam 2 Full v3 Bio200 Win16

... In the diagram, a snapshot of a single chromosome is shown along with RNA polymerase and ribosomes. There are RNAs of various sequences, as well as three different proteins. Use this diagram to answer the questions on pages 2-3. /5 1a) At the moment of this snapshot shown to the right, which of the ...
Chapter 24 Genes and Chromosomes
Chapter 24 Genes and Chromosomes

... Average protein 350 AA (1050 bp) Eukaryote and some prokaryote have noncoding DNA in middle to make even longer How many genes in a Chromosome? Ecoli genome is a single chromosome that has now been completely sequenced 4,639,675 bp 4,300 genes for proteins 157 genes for structural or catalytic RNA H ...
Manual: XL1-Blue Supercompetent Cells
Manual: XL1-Blue Supercompetent Cells

the description of the course
the description of the course

... Description of course: ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) e-ISSN: 2278-3008, p-ISSN:2319-7676.
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) e-ISSN: 2278-3008, p-ISSN:2319-7676.

... heating the DNA, exposing it to ultraviolet light, or subjecting it to the activity of an appropriate enzyme. Single strands of DNA have the property that they will spontaneously attach themselves to complementary strands to form the normal double-stranded structure. This behaviour persists even whe ...
Biological Sequences: DNA, RNA, Protein
Biological Sequences: DNA, RNA, Protein

... DNA molecule in simple life forms (e.g. bacteria). Eukaryotic cells have many chromosomes. In addition to the nucleus, mitochondria and chloroplasts have their own DNA sequences that encode for the proteins and RNAs unique to those organelles. • RNA occurs in multiple copies and various forms. Cells ...
Molecules and morphology: where`s the homology?
Molecules and morphology: where`s the homology?

... four recombinase subunits. Two subunits bind at a specific 'core' region of about 30 bp in each of the two recombining sites; these core regions usually have identical sequences. There are at least two different ways of exchanging the DNA strands to make recombinants (Fig. la,b) 1. One large family ...
14–3 Human Molecular Genetics
14–3 Human Molecular Genetics

... DNA fingerprinting analyzes sections of DNA that have little or no known function but vary widely from one individual to another. Only identical twins are genetically identical. DNA samples can be obtained from blood, sperm, and hair strands with tissue at the base. ...
013368718X_CH10_143-158.indd
013368718X_CH10_143-158.indd

... RNA Synthesis Most of the work of making RNA takes place during transcription. In transcription, segments of DNA serve as templates to produce complementary RNA molecules. In prokaryotes, RNA synthesis and protein synthesis takes place in the cytoplasm. In eukaryotes, RNA is produced in the cell’s n ...
Simulating Protein Synthesis to create a CHNOPS! Read the
Simulating Protein Synthesis to create a CHNOPS! Read the

... Simulating Protein Synthesis to create a CHNOPS! Read the following to help you complete a successful CHNOPS organism.  Genes are the units that determine inherited characteristics such as hair color as blood type. Genes consist of DNA molecules that code for the proteins our cells make. The sequen ...
Making the connection: DNA to Protein Engagement Exploration
Making the connection: DNA to Protein Engagement Exploration

... • Genes are segments of DNA molecules. Inserting, deleting, or substituting DNA segments can alter genes. An altered gene may be passed on to every cell that develops from it. The resulting features may help, harm, or have little or no effect on the offspring's success in its environment (5B, Grades ...
Biology 30 - Patricia Schwandt Courses
Biology 30 - Patricia Schwandt Courses

... If there were only one, there could only be four codes, representing only four different amino acids. How many different codons can be made if there are two nucleotides in each, for example, AA, AT, AC, AG, and so on? Will this be enough to represent all 20 amino acids? Since it is only possible to ...
Molecular Biology
Molecular Biology

... • Many enzymes contain more than one polypeptide chain and each polypeptide is usually encoded in one gene • These observations have lead to the one gene one polypeptide hypothesis: Most genes contain the information for making one polypeptide ...
Transcription & Translation - mvhs
Transcription & Translation - mvhs

... • Genes are “coding” regions of DNA – Each gene is the code for how to make a specific protein. ...
L 04 _transcription
L 04 _transcription

... Not all genes encode polypeptides: rRNA and tRNA genes encode RNA molecules. Not all genes occur only once in the genome: rRNA genes occur in clusters of dozens to hundreds of tandemly repeated copies, called rDNA. In humans, rDNA is on chromosomes 1, 13, 14, 15, 21, and 22. Recombination between rD ...
Lecture 1 - Portal UniMAP
Lecture 1 - Portal UniMAP

... • Ability to explain foundations of modern biotechnology. • Ability to demonstrate important recent advances in methods and applications of biotechnology with regards to microorganisms and plants. • Ability to differentiate scopes and importance of various ...
What is DNA? - Livingstone High School
What is DNA? - Livingstone High School

... What are the limitations of the model? •Each square represents one gene on one of the 23 chromosomes. •Each gene is not selected individually – they come in packets called ____? •Human traits like eye color and hair color have multiple alleles – they are the results of one gene from the mother and o ...
DNA Replication in Bacteria
DNA Replication in Bacteria

... In prokaryotes replication proceeds at about 1000 nucleotides per second, and thus is done in no more than 40 minutes. ...
Agrobacterium Plasmid Prep
Agrobacterium Plasmid Prep

... rolling the tube. The solution should become clear and translucent due to bacterial lyses. Let stand (max. 1/2 hour) at RT if solution fails to clear. (Note: Solution #2 will still work if several weeks old; however, the fresher it is, the better your prep will be. It is not recommended to use if ol ...
Exam 3 Review -Key - Iowa State University
Exam 3 Review -Key - Iowa State University

... 47. What is RNA interference and what happens during this process? - RNA interference is a form of gene regulation that directly prevents mRNA from undergoing translation. - RNA polymerase is going to transcribe genes that code for RNAs that double back on themselves to form a hairpin structure. - T ...
Proceedings - Applied Reproductive Strategies in Beef Cattle
Proceedings - Applied Reproductive Strategies in Beef Cattle

... DNA testing can increase accuracy of selection in a shorter amount of time than can be achieved by progeny testing. The improved accuracy of selection will result in faster genetic gains. Producers must also understand the limitations of these tests. No DNA test can explain all of the genetic variat ...
What are KPC and other CRE?
What are KPC and other CRE?

خلف زيدان قدوري .م كلية تكريت جامعة – االسنان طب
خلف زيدان قدوري .م كلية تكريت جامعة – االسنان طب

... structures with three or four strands can form. Nucleic acids are linear polymers (chains) of nucleotides. Each nucleotide consists of three components: a purine or pyrimidine nucleobase (sometimes termed nitrogenous base or simply base), a pentose sugar, and a phosphate group. The substructure cons ...
Gene therapy - MsSunderlandsBiologyClasses
Gene therapy - MsSunderlandsBiologyClasses

...  Create double-stranded DNA copies of their RNA genomes  These copies of its genome can be integrated into the chromosomes of host cells  Adenoviruses  Have double-stranded DNA genomes that cause respiratory, intestinal, and eye infections in humans. ...
< 1 ... 282 283 284 285 286 287 288 289 290 ... 766 >

Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report