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DNA
DNA

... Due to the various properties of the amino acid side chains, certain residues are found more often in one or the other structural units. Some residues have been classified, for example, as α-helix breakers. Proline for example is a poor helix former due to the fact that its backbone N atom is alread ...
integration from proteins to organs: the physiome project
integration from proteins to organs: the physiome project

XRCC1 (X-Ray Repair Cross Complementing
XRCC1 (X-Ray Repair Cross Complementing

... Cat. #MS-434-PCL (0.1ml) (Positive Control for Western Blot) rad4/cut5 gene. Nucleic Acids Research, 1993, ...
vocab-genetics - WordPress.com
vocab-genetics - WordPress.com

... Define the terms homologous, haploid / monoploid and diploid with respect to chromosomes. ...
chapter 19 the organization and control of eukaryotic genomes
chapter 19 the organization and control of eukaryotic genomes

... Eukaryotic repressors can cause inhibition of gene expression by blocking the binding of activators to their control elements or to components of the transcription machinery or by turning off transcription even in the presence of activators. ...
Name
Name

... D) the letter X. E) a twisted rope ladder. 20) If one strand of DNA is CGGTAC, the corresponding strand would be (C.1.a) A) GCCAUC B) TAACGT C) CGGTAC. D) GCCTAG E) GCCATG 21) DNA replication (C.1.a) A) uses each strand of a DNA molecule as a template for the creation of a new strand. B) occurs thro ...
PDF sample
PDF sample

... swoop in to be paired up correctly with the lonely bases there. Adenines are connected to thymines; cytosines are paired with guanines. ...
-u o DNA RECOVERY METHOD COMPARISON from BLACK
-u o DNA RECOVERY METHOD COMPARISON from BLACK

Bio-Tech - AgriLife Extension County Offices
Bio-Tech - AgriLife Extension County Offices

... Biotechnology is the manipulation of living organisms to produce products, processes, and services that are beneficial to mankind. Biotechnology can be used to produce more abundant and nutritious food products, pest and disease resistant crops, and even pharmaceuticals that can be used to treat dis ...
Questions - National Biology Competition
Questions - National Biology Competition

... C Use your time effectively. Do not spend too much time on questions that are too difficult. Go on to other questions and come back to the difficult ones later if you have time. It is not expected that everyone will be able to answer all questions. C Good luck and have fun! ...
What is RNA? - Biology for Life
What is RNA? - Biology for Life

... • Unlike DNA, most RNA molecules are singlestranded and can adopt very complex threedimensional structures. ...
Document
Document

... mutation. A somatic mutation affects only the somatic cell in which it originated and all of the daughter cells that the somatic cell produced. If a somatic mutation occurs early during embryonic development, it may affect a fairly large region of the organism. Since germ-line mutations affect the e ...
Mendelian Genetics I: Ratios
Mendelian Genetics I: Ratios

... 1. Is cell the correct size? Is DNA damaged? 2. Is DNA fully replicated? Is DNA damage repaired? 3. Have spindle fibers formed? Have they attached to chromosomes correctly? ...
DNA Technology
DNA Technology

... 12.6 Recombinant cells and organisms can mass-produce gene products  Recombinant cells and organisms constructed by DNA technologies are used to manufacture many useful products, chiefly proteins.  Bacteria are often the best organisms for manufacturing a protein product because ...
C1. A. G→A, which is a transition. B. T→G, which is a transversion. C
C1. A. G→A, which is a transition. B. T→G, which is a transversion. C

... mutation. A somatic mutation affects only the somatic cell in which it originated and all of the daughter cells that the somatic cell produced. If a somatic mutation occurs early during embryonic development, it may affect a fairly large region of the organism. Since germ-line mutations affect the e ...
Exam II
Exam II

... The numbers below the top map (173, etc) are the number of nucleotides between genes. a. (2 pts.) The original study showed that a regulatory protein binds a site overlapping the argF promoter. Part of their evidence was a footprint experiment, which shows protection of a 29 base region, which exten ...
Casework Genetics Uses Illumina Technologies to Decipher
Casework Genetics Uses Illumina Technologies to Decipher

... Genetics Enters the Forensics Laboratory Since the mid-1990’s, short tandem repeat (STR) polymorphism analysis has been used for genetic fingerprinting in forensic cases. The STRs used for forensic testing are most commonly tetranucleotide repeats, where a pattern of four bases is repeated multiple ...
Eurofins` adaption and optimisation software “GENEius” in
Eurofins` adaption and optimisation software “GENEius” in

... of transcription and translation in E.coli. And last but not least, GFP5 have been chosen for gene synthesis and were used in the the GC content is equally distributed to avoid GC-rich subse- expression experiments quences within in the gene. All these parameters are taken into account and a score i ...
Document
Document

... MEME represents motifs as position-dependent letterprobability matrices which describe the probability of each possible letter at each position in the pattern. Individual MEME motifs do not contain gaps. Patterns with variable-length gaps are split by MEME into two or more separate motifs. MEME take ...
epigenetics of carcinogenesis
epigenetics of carcinogenesis

... In this study, we for the first time found that low dose radiation (LDR) exposure causes profound and tissue-specific epigenetic changes in the exposed tissues We established that LDR exposure affects methylation of repetitive elements in the genome, causes changes in histone methylation, acethylati ...
Enhancer
Enhancer

Genetics - National Multiple Sclerosis Society
Genetics - National Multiple Sclerosis Society

... They contain the recipes, or instructions, for making the proteins of which all living things, from bacteria to humans, are built and which all organisms use to carry out their functions. Since the 1970s, scientists have been developing a set of tools—the methods of molecular genetics—that can isola ...
Cloning genes into the AdZ vectors and making
Cloning genes into the AdZ vectors and making

... (rendering the vector non-replicative) in a single copy vector. It contains the HCMV major immediate early promoter & PolyA signal sequence, with a cassette in the MCS encoding ampicillin resistance, lacZα & SacB. SacB encodes for a gene giving sensitivity to sucrose, lacZα provides blue/white scree ...
Recombinant DNA technology
Recombinant DNA technology

... Targeting strategy for apoE4 knock-in mice and homologous intergration of the transgene. (A) Schematic diagram of the knock-in targeting strategy. (Top) The structure of the endogenous Apoe locus including exons 1–4 (black boxes). (Middle) The targeting vector containing the human apoE4 cDNA (hu cDN ...
The Flow Cell: The Sequencer:
The Flow Cell: The Sequencer:

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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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