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Regulating Gene Expression
Regulating Gene Expression

... Under normal conditions, the lysine tails of histones extend out from the nucleosome and are attracted to other nucleosomes Histone acetylation attaches acetyl groups to these tails, making them no longer attracted to other histones, which loosens up the chromatin to make transcription easier It’s a ...
Introduction to Pharmacogenetics Competency
Introduction to Pharmacogenetics Competency

... alleles for a particular gene from each parent • A patient may be called homozygous based on either their genotype or predicted function of the alleles inherited • Homozygous may refer to a patient inheriting the same allele for a particular gene from both parents http://ghr.nlm.nih.gov/glossary ...
MS Word worksheet
MS Word worksheet

... Be able to use the codon table to construct the genetic code for a polypeptide chain (assuming you are given the amino acid sequence of the polypeptide). ...
Chapter 4B
Chapter 4B

... An overview of semiconservative replication is presented in Fig. 4.30. The event depicted is occurring at a replication fork formed after replication has initiated at a replication origin. One strand of the lower daughter molecule (the leading strand) is being synthesized continuously in the same di ...
Simple Life Forms: an Oxymoron “Then God said, “Let the land
Simple Life Forms: an Oxymoron “Then God said, “Let the land

... that can be read like a book. Every cell in the human body contains these same DNA strands. Therefore each cell has about three billion DNA base pairs comprising an estimated 20,000-25,000 genes. Compare that to an average page of text of 2,000 to 2,500 letters. Just the DNA letters in each individu ...
What Is the Genetic Code? 1. Explain, in general terms, how the
What Is the Genetic Code? 1. Explain, in general terms, how the

... Be able to use the codon table to construct the genetic code for a polypeptide chain (assuming you are given the amino acid sequence of the polypeptide). ...
protein synthesis
protein synthesis

... 1 codon codes for 1 amino acid (A.A.) There are 4 bases (A, T, C, G) Thus 4 3 (64) possible combinations of codons There are 20 amino acids Code is redundant (2 or more codons code for same amino acid) but not ambiquous (no codon codes fro more than 1 amino acid) ...
Handout
Handout

... paring of the bases (A-T, G-C) having one strand after separation could produce a complimentary strand ...
RNA & Protein Synthesis
RNA & Protein Synthesis

... ones break off leaving just amino acids bonded to each other  This continues until one of the three STOP codons is met  Finished amino acid strand goes through protein folding ...
Isolation of a UV Endonuclease from the
Isolation of a UV Endonuclease from the

... The exact reaction conditions were important when carrying out UV endonuclease analysis. Since crude extracts were being used as a source of endonuclease, it was possible that these extracts could contain photoreactivating enzyme which would also have activity towards the dimer substrate. Photoreact ...
AP Biology Chapter 20 Biotechnology Guided Notes
AP Biology Chapter 20 Biotechnology Guided Notes

... • Gene expression can also be silenced using RNA interference (RNAi) • Synthetic double-stranded RNA molecules matching the sequence of a particular gene are used to break down or block the gene’s mRNA ...
Enteric bacteria as model systems
Enteric bacteria as model systems

...  Alternatively, we can use a reporter gene to tell us how genes are expressed. Reporter genes have well characterized products that are easy to assay. The task is to place reporter gene expression under control of the raf promoters.  This can be accomplished in many ways  First, we can use transp ...
An easy-to-use, web-based DNA annotation platform
An easy-to-use, web-based DNA annotation platform

20_Lecture_Presentation_PC
20_Lecture_Presentation_PC

... to genetic engineering, the direct manipulation of genes for practical purposes • DNA technology has revolutionized biotechnology, the manipulation of organisms or their genetic components to make useful products • An example of DNA technology is the microarray, a measurement of gene expression of t ...
MUTATIONS
MUTATIONS

... Duplication: are chromosomal changes in which one copy or more copies of a gene are present on the same chromosome ...
Site-Directed Mutagenesis Using Oligonucleotide
Site-Directed Mutagenesis Using Oligonucleotide

Introduction - Cedar Crest College
Introduction - Cedar Crest College

... Wild-type strains of Neurospora (“original eaters,” or prototrophs) were grown on a minimal nutritional medium consisting of sucrose, minerals, and a few vitamins. ...
Biology Keystone Exam Review
Biology Keystone Exam Review

... (i.e. crossing over, nondisjunction, duplication, translocation, deletion, insertion, and inversion). What is crossing over, and when does it occur during meiosis? Crossing over is the exchange of genetic information from two non-sister chromatids during prophase I of meiosis Distinguish between a c ...
Activity 1: How Mendel`s Pea Plants Helped Us With Genetics You
Activity 1: How Mendel`s Pea Plants Helped Us With Genetics You

... 7) These "sentences" are called ________________. Activity 3: What is a chromosome? http://learn.genetics.utah.edu/content/basics/ Click on “What is a chromosome?” 1) If you stretched the DNA from a cell out, how long would it be? 2) How many chromosomes are in a human cell? In a mosquito? In a carp ...
Untitled
Untitled

... Complete removal of PCR inhibitors. High quality DNA that can be directly used in PCR, Southern, any enzymatic reaction, cloning, etc. ...
Heredity Unit Tic-Tac-Toe
Heredity Unit Tic-Tac-Toe

... Stem cells are undifferentiated or working together to develop breeding in order to improve ‘blank’ cells found in the human genetically modified (GM) foods. certain dog breeds. Choose two body that have the potential to Create a news report that explains species to combine through develop into many ...
DNA Sequence Analysis Using Boolean Algebra
DNA Sequence Analysis Using Boolean Algebra

... information to be an “a periodic crystal”: a structured medium (crystal) capable of storing information because of variation allowed within the structure (a periodicity)[5]. With more and more complete genomes of prokaryotes and eukaryotes becoming available and the completion of human genome projec ...
D. melanogaster
D. melanogaster

... human populations is highly variable and is controlled by a polymorphic element cis-acting to the lactase gene. A putative causal nucleotide change has been identified and occurs on the background of a very extended haplotype that is frequent in Northern Europeans, where lactase persistence is frequ ...
Patterns of Inheritance 10 Grade - Delaware Department of Education
Patterns of Inheritance 10 Grade - Delaware Department of Education

... disorder resulting from a mutation on chromosome 15. This mutation causes a fatty substance to build up in the nerve cells of the brain, resulting in a deterioration of brain function that ultimately leads to paralysis and death before the age of 5. A person with Tay Sachs disease lacks a protein (e ...
Cellular Gate Technology
Cellular Gate Technology

... Proteins are ordered molecular polymers of 50-1000 amino acids, of 20 different types. Each of the approximately 500-10,000 protein types in a typical cell consists of a unique sequence of amino acids. Moreover, each protein chain folds into a characteristic threedimensional structure, which is nece ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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