• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Structural analysis of both products of a reciprocal translocation
Structural analysis of both products of a reciprocal translocation

... The occurrence of reciprocal translocations between the c-myc gene and the Imraunoglobulin loci In Burkitt lymphoma provides a rare opportunity to analyze both the substrates and the products of a reciprocal recombination that occurs In a human cell (1-3). Such analyses are Important because they r ...
Quiz 2 Review Sheet
Quiz 2 Review Sheet

... for mRNA, tRNA or rRNA? 118. How are tRNA’s and rRNA’s made? 119. Describe the structure of the ribosome. What is it made of? 120. Where do the amino acids that our cells use to build polypeptides come from? Yes, I know they are in the cytoplasm, but before that… 121. List each macromolecule class a ...
Monday, Oct - Fall Pima 100
Monday, Oct - Fall Pima 100

... We are at the beginning of a personal-genomics revolution that will transform not only how we take care of ourselves but also what we mean by personal information. In the past, only élite researchers had access to their genetic fingerprints, but now personal genotyping is available to anyone who ord ...
Recombinant DNA and Gene Cloning
Recombinant DNA and Gene Cloning

... 3. Col plasmids:They code for colicins. These colicins are proteins that kill other bacteria e.g. colE1 of E.Coli. 4. Degradative plasmids: They allow the host bacterium to metabolise unusual molecules such as Toluene and Salicylic acid e.g. TOL of Plasmodium putida. 5. Virulence plasmids: These con ...
Biology Junction
Biology Junction

... DNA Fingerprinting DNA fingerprinting analyzes sections of DNA that have little or no known function but vary widely from one individual to another. Only identical twins are genetically identical. DNA samples can be obtained from blood, sperm, and hair strands with tissue at the base. ...
Document
Document

... cloned using molecular techniques. Reverse genetics uses the opposite sequence of steps. The gene is cloned first, and a phenotype for the gene (based on the creation of a gene knockout) is discovered later, by making a transgenic animal with a gene knockout. ...
495-Ze15
495-Ze15

... results only, the results are more physically based ones as compared to those obtained by MM methods, but such results can be obtained for rather simple systems only. Combined use of these two methods enables constructing atom level models of elementary biological processes, including the most impor ...
14–3 Human Molecular Genetics
14–3 Human Molecular Genetics

Genetics 314 – Spring, 2005
Genetics 314 – Spring, 2005

... 3. You want to express the DNA sequence in bacteria. Your friend says you need to add additional sequences to get expression. What sequences do you need to add and what are they needed for to allow expression of the DNA sequence in bacteria? ...
13-2 Manipulating DNA
13-2 Manipulating DNA

... These bands can then be compared with other samples of DNA. ...
- North Clarion County School District
- North Clarion County School District

... would look like.  They were also all tall.  He called them heterozygous because he knew that had 2 unlike genes for a trait. ...
File
File

... layers of organic matter in sediment into natural gas, coal, and petroleum. When fossil fuels are burned, they release a gas that can be used by plants for photosynthesis. High levels of this gas in the atmosphere are typically associated with warmer periods on Earth. What greenhouse gas is relea ...
Document
Document

... 1. Initiation: DNA is unzipped and the enzyme RNA polymerase runs along the template strand of the DNA. – The template strand of DNA can be identified by finding the promoter region: nucleotide sequence T A C at the 3’ end (If the strand is written backwards it may look like C A T at the 3’ end). Th ...
Biology Notebook
Biology Notebook

... coat (capsid) of the virus. It “tells” the genetic sequence of the virus, to the cells of the organism that is infected.  Antibiotics cannot destroy viruses, because viruses are metabolically inert, they aren’t alive.  They were discovered by Edward Jenner in order to fight against smallpox. ...
What is trans-acting factor?
What is trans-acting factor?

... protein. Example: the activator Gal4 is controlled by the masking Gal80). Some masking proteins not only block the activating region of an activator but also recruit a deacetylase enzyme to repress the target genes. Example: Rb represses the function of the mammalian transcription activator E2F in t ...
DNA Fingerprinting
DNA Fingerprinting

... The Human Genome Project has provided information to link the identification of many types of cancers and other diseases to DNA sequence information. (Edvotek) Cancer has been found to be linked to mutations in a tumor suppressor genes such as one called p53. These genes usually keep cells from divi ...
WORD
WORD

... 1973 American geneticist Stanley Cohen and American biochemist Herbert Boyer removed a specific gene from one bacterium and inserted it into another using restriction enzymes. This event ...
IBC Form 1 - Grinnell College
IBC Form 1 - Grinnell College

... viruses when propagated only in that host (or a closely related strain of the same species), or when transferred to another host by well established physiological means. 6. Those that consist entirely of DNA from a eukaryotic host including its chloroplasts, mitochondria, or plasmids (but excluding ...
REVIEW for the Spring Final Fill In
REVIEW for the Spring Final Fill In

... 3. If 14% of a sample of DNA is cytosine, what percentage of the DNA is thymine? 4. In the following diagram, determine what each label is pointing to. What is this process called? _________________ A= B= C= D= E= F= 5. Define: replication transcription translation haploid diploid independent assort ...
Going Through the Motions_putonwiki
Going Through the Motions_putonwiki

... b. What does this sequence for the 5’splice junction always start with? c. What is the purpose of the 5’splice junction? d. What is another name for the 3’ splice junction? e. What does this sequence always have and end with? f. What is the purpose of the 3’ splice junction? g. Find our intron(s) by ...
trial by probability: bayes` theorem in court - UW
trial by probability: bayes` theorem in court - UW

... seen in the area after many of the murders. Finding records of a 1977 white corvette getting a traffic violation in the area obtained a name along with a DNA test. After some further questioning, a man was arrested and charged with 12 murders dating back to 1990. This number could still increase to ...
Where Is DNA Found?
Where Is DNA Found?

...  Heat the DNA strands, causing the strands to separate (unzip).  Cool the mixture and add a primer, a short sequence of base pairs that will add to its complementary sequence on the DNA strand.  Finally, add a DNA polymerase and a mixture of free nucleotides to the separated strands. Heat again t ...
The New Genetics of Mental Illness
The New Genetics of Mental Illness

What is a gene?
What is a gene?

Exp DAV Spike protein
Exp DAV Spike protein

... • Adenoviruses infect wide variety of animals – Wild & Domestic – Pathogenic in Deer – Symptoms of AV are ulcers and abscesses in the mouth and throat – Acute Symptoms would be rapid breathing, diarrhea, foaming at the mouth – Death can occur with 3-5 days from the time of the exposure. – No known c ...
< 1 ... 266 267 268 269 270 271 272 273 274 ... 766 >

Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report