• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Document
Document

... Immediately after determining the structure of DNA (1953), Watson and Crick proposed what is known as the semi-conservative model of DNA replication, and they happened to be correct although they would now know this until experiments done by American geneticists Meselson and Stahl in 1958… ...
Permutation Representation
Permutation Representation

... Child 1 = α.xi + (1-α).yi and Child 2 = α.yi+ (1-α).xi  If α=1/2 the two offsprings will be identical for this operator ...
Processivity of DNA polymerases: two mechanisms, one goal
Processivity of DNA polymerases: two mechanisms, one goal

... the one in T7 polymerase between helices H and H1. The polymerase of the E. coli bacteriophage T3 contains a thioredoxin-binding domain and thus may use thioredoxin as a processivity factor in a similar manner to T7. Similarly, the DNA polymerase of the Bacillus subtilis bacteriophage Spo1 also cont ...
Chapter 17: From Gene to Protein 1. Overview of Gene Expression 2. Transcription
Chapter 17: From Gene to Protein 1. Overview of Gene Expression 2. Transcription

... How are Genes related to DNA? Genes are segments of DNA that code for a particular protein (or RNA molecule) • the human genome contains ~3 billion base pairs (bps) and ~25,000 genes • most genes encode proteins • when we talk about “genes” we will focus on those that express proteins ...
IBS 602 - Office of Biomedical Education
IBS 602 - Office of Biomedical Education

... original grading. Resubmissions must be accompanied by a written explanation of the perceived mistake. Upon resubmission, the entire examination will be subject to reevaluation and all questions therein will be regraded. Examinations for regrading must be submitted within one week (7 days) and to th ...
Section Title – One Line Preferred, Two Line Maximum
Section Title – One Line Preferred, Two Line Maximum

... STRs and SNPs are useful in DNA-based forensic investigations, missing persons investigations and paternity disputes. ...
An Introduction to Genetic Analysis Chapter 14 Genomics Chapter
An Introduction to Genetic Analysis Chapter 14 Genomics Chapter

... nature of whole genomes; and functional genomics, characterizing the transcriptome (the entire range of transcripts produced by a given organism) and the proteome (the entire array of encoded proteins). The prime directive of structural genomic analysis is the complete and accurate elucidation of th ...
Supplemental figure 1 Complete CLSM stacks of Ad3 texas
Supplemental figure 1 Complete CLSM stacks of Ad3 texas

... the Eco RV-linearised form of the three plasmids ppolyAd∆EP-TETP, ppoly-Ad∆EPTETP-∆24, ppoly-Ad∆EP-TETP-∆24∆19 and a modified form of the 28 kb Cla I- Pac I fragment containing a Swa I restriction site inserted into the deleted fiber region 7. The second recombination was between the Swa I-linearise ...
Information S1.
Information S1.

... numbered vials consisting of the dilutions in triplicates for each cell line DNA and 12 aliquots of SW46 DNA. A total of 3600 samples were sent to 40 laboratories. Extraction, dilution and distribution were centralized in one laboratory (UMR775, INSERM, Pr P. Laurent-Puig). The p.G12R cell line obta ...
16_Lecture_Presentation
16_Lecture_Presentation

... various proteins within the white blood cells. • he came across a substance from the cell nuclei that had chemical properties unlike any protein, – -a much higher phosphorous ...
BIOLOGY 2013-‐2014 FINAL EXAM STUDY GUIDE
BIOLOGY 2013-‐2014 FINAL EXAM STUDY GUIDE

... Explain  what  a  cell  plate  does  and  where  it  would  be  found   ...
Vocabulary: Did you know?
Vocabulary: Did you know?

... Genotype-­‐  Your  genotype  is  the  composition  of  alleles  you  have  for  a  particular   gene  or  genes.  Remember  than  many  genes  come  in  two  or  more  different  “flavors”   or  alleles—one  version  (or  allele)  may ...
Protein Synthesis – Part 3
Protein Synthesis – Part 3

... mRNA Synthesis and Modification (The making of mRNA.)(This process occurs at the nucleolus. Remember, the nucleolus is “like” a copy machine because we are making a cheap disposable copy of the DNA sequence.) A. Three Phases of Production to a transcription unit (Piece of mRNA.): 1. Initiation (This ...
ANSWER - EdWeb
ANSWER - EdWeb

... 76. Based on the descriptions below, identify the correct genetic disorder. ANSWER: a. Individual may have both female and male characteristics because their sex chromosomes are XXY = ...
Chapter 9
Chapter 9

... • Cut specific sequences of DNA • Destroy bacteriophage DNA in bacterial cells • Methylated cytosines in bacteria protect their own DNA from digestion • Create blunt ends or staggered cuts known as sticky ends ...
PPT Version
PPT Version

... Fast RA • Generate a token based on the link-local address of the routers on the link • A host token is generated using the source address of the RS message • Ranking is decided based on the XOR of the host token and the router tokens • FastRAThreshold controls the number of fast RA messages • Toke ...
as a PDF
as a PDF

... In many bird species, the sex determination is very difficult in young birds and many adults. Nowadays, sex identification of animals throughout their lives is possible by molecular genetic techniques. In the present study, the sex identification of canaries by PCR methods based on chromo helicase D ...
Biology I ECA Review Standard 7 Genetics
Biology I ECA Review Standard 7 Genetics

... allelic and polygenic traits and illustrate their inheritance patterns over multiple generations.  7.3 Determine the likelihood of the appearance of a specific trait in an offspring given the genetic make-up of the parents.  7.4 Explain the process by which a cell copies its DNA and identify facto ...
Document
Document

... 1. I would use mixed selection criteria because I think they are more biological: a. Baseline allocation of CPU time is independent of ...
LIFE SCIENCES – 2003 1) Which of the following processes require
LIFE SCIENCES – 2003 1) Which of the following processes require

new lab 9 chromosomal map
new lab 9 chromosomal map

... Chromosome map unit : Unit of map distance between genes , and is termed ...
Animals and plants manage to make copies of themselves from one
Animals and plants manage to make copies of themselves from one

... been isolated and purified have been found to be made up of protein. For his work, Stanley won a share of the Nobel Prize for chemistry in 1948. Almost at once it was found that viruses contain more than protein. In 1937, an English biologist, Frederick Charles Bawden (1908- ) found that the tobacco ...
Colony PCR from Yeast or Bacteria
Colony PCR from Yeast or Bacteria

Mechanisms and impact of genetic recombination in the evolution of
Mechanisms and impact of genetic recombination in the evolution of

... In bacteria, uptake of exogenous DNA is achieved via three main processes; transformation, conjugation and transduction. Bacterial conjugation occurs when there is direct cell-to-cell contact of the bacteria exchanging DNA via a sex-pilus that protrudes from one cell into the other. During transduct ...
7 Molecular Genetics: From DNA to Proteins
7 Molecular Genetics: From DNA to Proteins

... In the early 1940s, a team of scientists led by Oswald Avery tried to answer the question raised by Griffith’s results. They inactivated various substances in the S-strain bacteria. They then killed the S-strain bacteria and mixed the remains with live R-strain bacteria. (Keep in mind, the R-strain ...
< 1 ... 185 186 187 188 189 190 191 192 193 ... 766 >

Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report