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Nucleic Acid Lateral Flow Immunoassay for the Detection of
Nucleic Acid Lateral Flow Immunoassay for the Detection of

... more popular for testing of wide range of analytes. The lateral flow tests offers many benefits (user-friendly format, short time to get test result, long-term stability, and relatively low price). A new promising application is the detection of genetic material, e.g. various genetic markers, DNA or ...
Molecular Biology (Ms. Lucky Juneja)
Molecular Biology (Ms. Lucky Juneja)

... Most mutations affect only one base pair in a given location and therefore are called point mutations. There are several types of point mutations: 1.Silent mutation: If a mutation is an alteration of the nucleotide sequence of DNA, mutations can occur and have no visible effect because of code dege ...
Chapter 9 Slide PDF
Chapter 9 Slide PDF

...  Translation requires the interaction of mRNA, amino acids, ribosomes, tRNA molecules, and energy sources  mRNA is read in groups of 3 amino acids called codons ...
Transcriptional Activation I
Transcriptional Activation I

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Local DNA stretching mimics the distortion caused by - ENS-phys

... interstrand separation between the phosphorus atoms at the 39 extremities of the TATA sequence. While the length of the strands (the two intrastrand distances) and the separation between the 59 extremities vary only slightly, the 39–39 distance changes from 13.8 Å in B-DNA to 20.0 Å in A-DNA and fin ...
Chapter 10
Chapter 10

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Gel electrophoresis of restriction digest
Gel electrophoresis of restriction digest

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An Introduction to Genetic Analysis Chapter 16 Mechanisms of Gene
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(FA-SAT) in a Cat Fibrosarcoma Might Be Related to Chromosomal

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Chapter 21: Genomics I: Analysis of DNA and Transposable Elements

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No Slide Title

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Probabilites on Pedigrees

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SLR-VK – 2

... b) All DNA damage results in diseases such as cancer c) All DNA damage is caused by physical, chemical or biological agents d) Most DNA damage is advantageous to the cell 3) What is the role of topoisomerases in eukaryotic DNA replication ? a) Topoisomerise enzymes cut, uncoil and reseal the double ...
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... fertilised egg divides time and time again this genetic material is replicated in each new cell. The sorting and recombining of genetic material (the process in which DNA is exchanged between chromosomes that contain the same sequence of genes) when egg and sperm cells are formed and then fuse resul ...
The sternum is to the arm. Lateral Medial Superior Inferior In what
The sternum is to the arm. Lateral Medial Superior Inferior In what

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Platinum DNA polymerases

... Figure 1. Relative fidelity values of different DNA polymerases. Polymerase fidelity was measured by next-generation sequencing. The background level of experimental errors was estimated from PCR-free library sequencing data. The polymerase fidelities were normalized to Taq polymerase. It is difficu ...
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HiPer® Real-Time PCR Teaching Kit

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An Introduction to Bioinformatics - E-Learning/An

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immunology of infections. hiv

... • Because of the ability of HIV to undergo recombination, the use of attenuated HIV virus as vaccine has been abandoned for now. • One promising approach is DNA vaccination, in which expression plasmids that drive expression of HIV protein subunits are introduced into host cells. • So far, all attem ...
Phenomena of Life and Death Based on Nonphysical Gene and
Phenomena of Life and Death Based on Nonphysical Gene and

... double helix by Francis Crick and James D. Watson in 1953 and in vitro characterization of the process of protein biosynthesis led to the idea that it was the linear sequence of ribonucleic acid derived from one of the DNA strands that directed the synthesis of a linear sequence of amino acids, or a ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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