• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
chapter 15 section 3 notes
chapter 15 section 3 notes

... No individual is exactly like any other genetically—except for identical twins, who share the same genome. Chromosomes contain many regions with repeated DNA sequences that do not code for proteins. These vary from person to person. Here, one sample has 12 repeats between genes A and B, while the se ...
A-level Biology Essay Titles Paper 3
A-level Biology Essay Titles Paper 3

... 10 (a) The membranes of different types of cells are involved in many different functions ...
Describe the central dogma of molecular biology.
Describe the central dogma of molecular biology.

...  Therefore, insertions or deletions that do not involve multiples of 3 base pairs will change the reading frame of the gene, and alter all codons downstream from the mutation. These are called frameshift mutations: ...
Ch. 5 LEcture PPt
Ch. 5 LEcture PPt

... D. Multiple Alleles- Some traits are determined by more than one allele. ...
Interaction
Interaction

... Oct-1 activates transcription of genes that are involved in basic cellular processes Oct-1 activates small nuclear RNA (snRNA) and ...
In experiments with a 3 base codon system it was shown that the
In experiments with a 3 base codon system it was shown that the

... cytoplasm and is similar to DNA since it also has four bases: A, U, G, C. ...
Chapter Introduction Lesson 1 Mendel and His Peas Lesson 2
Chapter Introduction Lesson 1 Mendel and His Peas Lesson 2

... • Mendel concluded that two factors—one from each parent—control each trait. • Mendel’s “factors” are part of chromosomes which exist as pairs—one chromosome from each parent. • Each cell in an offspring organism contains chromosomes from both ...
Teacher Background on Epigenetics 2013
Teacher Background on Epigenetics 2013

Is there a link between DNA and Obesity?
Is there a link between DNA and Obesity?

... molecule form a nucleotide. These nucleotides are arranged in long strands that form a double helix. The base pairs are the double helix’s stairs and the sugar phosphate molecules are the side pieces. DNA has a certain property of making exact copies of itself. This is important for bacteria or hea ...
CopyRight® v2.0 Fosmid Cloning Kit
CopyRight® v2.0 Fosmid Cloning Kit

... FOS vector are complete, without missing clones or deleted sequences. High DNA yields.The CopyRight v2.0 Fosmid vector and Replicator FOS cells feature inducible amplification of copy number**, increasing yields to as many as 50 copies per cell. CopyRight amplification is more robust than the simila ...
Final Exam answer key
Final Exam answer key

... 14. (4 pts) DNA markers are often designed around sites of DNA repeats of about 10100 base pairs. Why are these VNTR sites or minisatellites highly variable. Minisatellites can expand or contract if the sequences pair incorrectly in meiosis. 15. (8 pts) John Bowman studied the genetics of floral org ...
BioTeke Corporation Technical Manual
BioTeke Corporation Technical Manual

... 11. Transfer the Spin-column AC to a clean 1.5ml microcentrifuge tube, add 50μl Buffer EB (warm in 65-℃70℃ before use) directly onto the silicified membrane. Incubate 3-5 min at room temperature. Centrifuge at 13,000rpm at 1 min. The volume of elution buffer could be adjusted according to needs. Ap ...
PTK7 domain involvement in planar cell polarity
PTK7 domain involvement in planar cell polarity

... PTK7. If the anterior/posterior randomized selection of these CoPA neurons can be rescued to result in mostly anterior/wild type (not always 100% in real life) without one of PTK7’s three domains, then that domain can be concluded to not be required for regulating the PCP pathway in zebrafish CoPA ...
Improvement of DNA Extraction Protocols for Nostochopsis spp.
Improvement of DNA Extraction Protocols for Nostochopsis spp.

... polysaccharides of up to 49% w/w [3,6]. The polysaccharides, polyphenols and other secondary metabolites make it very difficult to isolate a satisfactory quality of DNA. These compounds bind tightly to nucleic acids during the isolation of DNA and interfere with other subsequent reactions [7]. Most ...
Cha. 3 Cell structure
Cha. 3 Cell structure

...  Organelles containing oxidative enzymes  H+ removed from toxic molecules – transferred to  Peroxide is formed ...
Document
Document

... More conventional examples in applying biotechnology Agriculture – produce fertilizers and pesticides Medicines – diagnose and prevent diseases, produce antibiotics, vaccine and drugs ...
Practise Midterm Exam
Practise Midterm Exam

... In the genomic DNA sequence shown above, draw boxes around the exons. ...
Overexpression of the Tryptophan Cluster in Corynebacterium
Overexpression of the Tryptophan Cluster in Corynebacterium

... biosynthetic pathway. The first enzyme gene and other L-tryptophan biosynthetic pathway genes form a operon and regulate by repressor system,the attenuator and feedback inhibition of L- tryptophan. And it is the key enzyme of L- tryptophan biosynthetic pathway. According to the attenuation model pro ...
Section J Analysis and Uses of Cloned DNA
Section J Analysis and Uses of Cloned DNA

... • The two main methods of DNA sequencing are (1) Maxam’s method: the end-labeled DNA is subjected to base-specific cleavage reactions prior to gel separation; (2) Sanger’s method: to uses di-dNTP as chain terminators to produce a ladder of molecules generated by polymerase extension of a primer. • A ...
CHAPTER 21
CHAPTER 21

... It would not account for the same number of A and T or C and G. It would not be able to explain replication. ...
DESKTOP YARN MITOSIS/MEIOSIS SET UP AHEAD OF TIME: Cut
DESKTOP YARN MITOSIS/MEIOSIS SET UP AHEAD OF TIME: Cut

... During S phase DNA is copied. Hold up each chromatin yarn piece and place a "copy" along side of it. G2 phase: The period after DNA synthesis has occurred but prior to the start of prophase. The cell synthesizes proteins and continues to increase in size ...
3.1 Genes - Peoria Public Schools
3.1 Genes - Peoria Public Schools

... sequence of a polypeptide in hemoglobin. The number of genes in a species should not be Comparison of the number of genes in humans with referred to as genome size as this term is used other species. for the total amount of DNA. At least one plant and one bacterium should be included in the comparis ...
Recombinant Technology
Recombinant Technology

... 12.9 DNA microarrays test for the expression of many genes at once • DNA microarray assays – Can reveal patterns of gene expression in different kinds of cells ...
I. Geometric Crossover
I. Geometric Crossover

lecture12-motif-finding
lecture12-motif-finding

... Regulatory regions are comprised of “binding sites” “Binding sites” attract a special class of proteins, known as “transcription factors” A TFBS can be located anywhere within the regulatory region (promoter region) Bound transcription factors can also inhibit DNA ...
< 1 ... 188 189 190 191 192 193 194 195 196 ... 766 >

Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report