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RNA to Protein
RNA to Protein

...  Three types of RNA are involved in translation: mRNA, rRNA, and tRNA  mRNA produced by transcription carries proteinbuilding information from DNA to the other two types of RNA for translation ...
Uses of DNA information on Commercial Cattle Ranches Alison Van
Uses of DNA information on Commercial Cattle Ranches Alison Van

Chapter 7 Microbial Genetics
Chapter 7 Microbial Genetics

... • Gene expression occurs when gene activity leads to a protein product in the cell (Protein Synthesis). • A gene does not directly control protein synthesis; instead, it passes its genetic information on to RNA, which is more directly involved in protein synthesis. • Difference between DNA and RNA • ...
Document
Document

... accuracy, errors do occur (< 1 error per billion bases copied) – mutations. • If cell survives the mutation, it will be copied every time DNA is replicated • Mutations cause diversity within species • Some mutations have no effect, some are lethal and in very rare occasions mutations are useful – ba ...
Cloning homework_S11
Cloning homework_S11

... 3. The melting temperature (Tm) should be between 55-80°C. (This is the temperature at which the primer will denature. Your annealing temperature in the PCR reaction needs to be below this temperature by at least 5°C and the two primers should not have extremely different Tm’s.) 4. Primers should en ...
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File

Understand the Basics of Genetic Testing
Understand the Basics of Genetic Testing

An Artist in Gene Editing - Max-Planck
An Artist in Gene Editing - Max-Planck

... An immune mechanism in bacteria, until recently known only to hardcore microbiologists, could soon become a medical treatment for millions of patients. CRISPR-Cas9 is therefore a prime example of one of Charpentier’s core beliefs: “Basic research is essential for progress,” she stresses at every opp ...
final exam in kje-2004
final exam in kje-2004

... and ProtC are likely to be unrelated to ProtB1 and ProtB2. ProtA is 61% identical to ProtC over 250 amino acids of length. This indicates that they are likely to be homologs. Short proteins with 61% identity on the other hand are not necessarily homologs even if they share a relatively high degree o ...
Bacterial Genetics
Bacterial Genetics

... -need for nutrients prototropic: can grow on minimal medium auxotropic: must have specific nutrients added to medium ...
Assignment 2
Assignment 2

... Based on the table of mRNA codons (see p33 in Relethford) answer the following questions: 1. Determine the direction of transcription 2. Locate the initiation and termination codons 3. Circle coding codons; cross-out non-coding areas 4. Draw a diagram showing the sequence of mRNA before and after sp ...
Genetics: Smoking out BRCA2
Genetics: Smoking out BRCA2

... When you're tuning a guitar, the smallest of tweaks can make a big difference to the way the instrument sounds. And in books, small changes in the way the words are put together can change how the story unfolds – little changes can have big effects. The same applies to DNA – the molecule that carrie ...
Analyzing the Changes in DNA Flexibility Due to Base Modifications
Analyzing the Changes in DNA Flexibility Due to Base Modifications

... issues. To strip water, we will first make a psf and pdb without water. We will then make an index file and feed the dcd and the index file into catdcd, which will produce a new dcd without water that takes up much less space. The vmd script, strip.tcl, accomplishes these tasks and takes as input a ...
Gene Transfer in Bacteria/Phage
Gene Transfer in Bacteria/Phage

An exo-b-( 1,3)-glucanase of Candida albicans
An exo-b-( 1,3)-glucanase of Candida albicans

... excised band by SDS-PAGE revealed only one protein of M, 42000. The present study and the recent report by Luna-Arias et al. (199 1) indicate that the secreted enzyme is monomeric. Edman degradation of the intact enzyme yielded 35 residues of the amino acid sequence and confirmed that the preparatio ...
Artemis as genome viewing and annotation tool
Artemis as genome viewing and annotation tool

... Overview of the genome sequencing and sequence analysis. Demonstration of Artemis. Hands on guided exercise in Artemis. Demonstration of ACT . Hands on guided exercise in ACT Generating ACT comparison files ...
Introduction to self-assembly Self
Introduction to self-assembly Self

... In such simulations, capsids will spontaneously assemble around a nucleic acid even if the NA’s charge significantly exceeds that of the capsid faces. A more important determinant is the typical volume of the NA, determined both by its length and its folding pattern. These simulations suggest anoth ...
SNPs
SNPs

... • Understand the genetics of diseases, esp. the more common complex ones such as diabetes, cancer, cardiovascular, and neurodegenerative. • To allow pharmaceutical treatments to be tailored to individuals (adverse reactions based on genetics). ...
Gene Tech answers622 KB
Gene Tech answers622 KB

... This task is linked with applying students’ knowledge of DNA sequencing and cloning. One possible application of this knowledge may be to sequence the DNA found in fossils of very rare species. This could make it possible to increase a rare population using cloning or even to resurrect an extinct sp ...
Biology II, Genetics - Southwest Allen County Schools
Biology II, Genetics - Southwest Allen County Schools

... • Explain the correlation between proper development and birth defects during the critical period pertaining to malfunctioning genes and environmental factors. • Explain the two basic laws of inheritance- segregation and independent assortment. • Understand basic terminology relating to Mendelian ge ...
Multiplexing of DELFIA® Cell Proliferation and DNA
Multiplexing of DELFIA® Cell Proliferation and DNA

... STS. The two assays do not significantly affect each other when performed simultaneously in the same sample wells. The data is expressed as signal to background for both the proliferation- and the DNA fragmentation assays when performed either alone or together. ...
Genome changes
Genome changes

... • A series of repeating units of 2 to 5 nucleotides is called a short tandem repeat (STR) • The repeat number for STRs can vary among sites (within a genome) or individuals • Simple sequence DNA is common in centromeres and telomeres, where it probably plays structural roles in the chromosome ...
Protein Synthesis PPT - Get a Clue with Mrs. Perdue
Protein Synthesis PPT - Get a Clue with Mrs. Perdue

... matching tRNA. 2. The codon of mRNA bases pairs to anti-codon of tRNA. 3. tRNA drops off amino acid to ribosome and then float away. 4. Ribosome pieces together amino acids to build proteins. ...
Sequences as arrays or strings
Sequences as arrays or strings

... 1 to reading frame 2 and the same when going from reading frame 2 to reading frame 3 subsequent ...
gen-305-presentation-8-16
gen-305-presentation-8-16

... Bacteriophages may also contain a sheath, base plate and tail fibers ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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