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DNA transcription
DNA transcription

... processes: transcription and translation. Transcription takes place in the nucleus. It uses DNA as a template to make an RNA molecule. RNA then leaves the nucleus and goes to a ribosome in the cytoplasm, where translation occurs. Translation reads the genetic code in mRNA and makes a protein. Transc ...
Supplementary Information (doc 38K)
Supplementary Information (doc 38K)

... and Choi (Hans et al. 2004; Choi et al. 2009). For this purpose the immunohistochemical expression of the different markers used (GCET1, CD10, MUM1, BCL6 and FOXP1) was scored by two independent pathologists (SMM and JADP) and the percentage of tumor-cell staining was estimated by visual inspection ...
Kodaq 2X PCR MasterMix
Kodaq 2X PCR MasterMix

... exceptional 3’ to 5’ exonuclease activity that endows it with superior accuracy over competitor polymerases. This novel enzyme has intrinsically high processivity and is engineered to have an improved binding affinity for DNA resulting in highly successful PCR. abm’s Kodaq 2X PCR MasterMix is a read ...
060506-Meeting
060506-Meeting

... • Conjugative plasmids are very large, from 60k – 100k basepairs long •The TraJ protein is a regulatory protein responsible for initiating the DNA transfer cascade •DNA transfer during conjugation always begins at a specific sequence on the plasmid, OriT, the Origin of Transfer. ...
Mutations File
Mutations File

- Environmental Biosafety Research
- Environmental Biosafety Research

... (1) Recombinant nucleic acid techniques involving the formation of new combinations of genetic material by the insertion of nucleic acid molecules produced by whatever means outside an organism, into any virus, bacterial plasmid or other vector system and their incorporation into a host organism in ...
Teacher`s guide - National Centre for Biotechnology Education
Teacher`s guide - National Centre for Biotechnology Education

... teaching of genetics and to integrate theory, technology and some ethical issues in one activity. Students are asked to determine the mode of inheritance of a genetic condition by analysing DNA samples that are said to come from individual members of the extended family. Restriction enzymes are used ...
Analysis of a piwi-related Gene Implicates Small RNAs in
Analysis of a piwi-related Gene Implicates Small RNAs in

... 1. The next slide illustrates (without data) an interesting property of the protein (Twi1p) encoded by the TWI1 gene. A tagged Twi1p that can rescue the conjugation lethal phenotype of a TWI1 deletion localizes first in the cytoplasm of conjugating cells. It then localizes exclusively in the old ma ...
Chromosomal Rearrangements I
Chromosomal Rearrangements I

... symbol used. Deletions can be located within a chromosome (interstitial) or can remove the end of a chromosome (terminal). Deletions can be small (intragenic), affecting only one gene, or can span multiple genes (multigenic). Deletions can arise from DNA damage (X-rays or chemical agents that break ...
Metabolic Managers
Metabolic Managers

... different shapes based on the number of amino acids, the type of amino acids, the sequence of amino acids. Ex. Analogy  26 letters of the alphabet  Form different words ...
Learning Log/ FRQ-style Question
Learning Log/ FRQ-style Question

... larger, it now requires more resources, like water and nutrients. To allow for the increased cotransport (process of using the effects of the H+ gradient to couple downhill passage of H+ with uphill passage of another solute into the cell) of such resources, the cell needs more cellular “equipment” ...
Mechanical separation of the complementary strands of DNA
Mechanical separation of the complementary strands of DNA

... involved in the elementary process of mechanical strand separation, with no enzyme present. The experiment presented here is approaching the Levinthal and Crane (5) configuration. Force measurement on single molecules of DNA is an emerging field (6–13). For typical molecular interactions involving b ...
Genetic Control of Cell Function and Inheritance
Genetic Control of Cell Function and Inheritance

... composed of the structural proteins and enzymes needed for protein synthesis. As with the other types of RNA, rRNA is synthesized in the nucleus. Unlike other RNAs, ribosomal RNA is produced in a specialized nuclear structure called the nucleolus. The formed rRNA combines with ribosomal proteins in ...
GPR17 shRNA Plasmid (r): sc-270023-SH
GPR17 shRNA Plasmid (r): sc-270023-SH

... concentrations of nucleotides and cysteinyl leukotrienes (CysLTs), two families of endogenous signaling molecules, increase significantly at the site of damage. In some neurons, GPR17, a membrane receptor for uracil nucleotide and CysLTs, is upregulated as well, infiltrating the lesioned area. GPR17 ...
Chapter 19 Nucleic Acids
Chapter 19 Nucleic Acids

... • In prokaryotes the primary mRNA transcript is translated directly • In eukaryotes transcription occurs in the nucleus, translation in the cytoplasm • Eukaryotic mRNA is processed in the nucleus without interfering with translation • In some mRNA, pieces are removed from the middle and the ends joi ...
제3회 한국분자세포생물학회 이동성 유전인자분과 학술대회
제3회 한국분자세포생물학회 이동성 유전인자분과 학술대회

... Ionizing radiation (IR) effects to genomic instability, which cause chromosomal aberrations, deletions, insertions, and point mutations, is considered to be the precursor of tumorigenesis. Even though effects on radiation exposure in cancer is very well-characterized by numbers of researchers, the u ...
3.2.U1 Prokaryotes have one chromosome consisting of a
3.2.U1 Prokaryotes have one chromosome consisting of a

... There is one copy of each gene except when the cell and its DNA are replicating. A copy is made just before the cell divides by binary fission ...
Molecular Cloning and Characterization of an
Molecular Cloning and Characterization of an

From Gene to Protein
From Gene to Protein

... b. 3, 1 c. 3, 3 d. 1, 20 The sugar in RNA is _____, the sugar in DNA is _______ a. deoxyribose, ribose b. ribose, deoxyribose c. ribose, phosphate d. ribose, uracil Which of the following is found on RNA but not DNA? a. uracil b. deoxyribose c. phosphate d. adenine A stretch of chromosome that codes ...
1. ELONGATION
1. ELONGATION

... In eukaryotes, the initial product of transcription, the primary RNA transcript, is processed in several ways before its transport to the cytosol. These processing steps are all performed by specific proteins that bind to the RNA. Until it reaches its final, mature form, the primary transcript is so ...
Strand 4 GM Antifreeze Gene 2010
Strand 4 GM Antifreeze Gene 2010

... product that includes animal DNA or not. Genetic pollution (pollen from GM crops may cross-pollinate non-GM crops and/or organic crops,rendering farmers unable to certify their crops as organic). Reduced genetic variation (farmers may opt to plant only the GM varieties to reap the benefits of th ...
CH 8. DNA: The Universal Molecule of Life
CH 8. DNA: The Universal Molecule of Life

RNA to Protein
RNA to Protein

...  Three types of RNA are involved in translation: mRNA, rRNA, and tRNA  mRNA produced by transcription carries proteinbuilding information from DNA to the other two types of RNA for translation ...
Teacher-submitted assessment ideas
Teacher-submitted assessment ideas

... Humans have evolved to be non-tasters. ...
Chapter 1
Chapter 1

... compounds, such as proteins or lipids. The lipids are mainly found in the membrane, which separates the inside of a cell from the outside world. Lipids, the third type of biopolymer, are not very soluble in water, and can form membrane-like structures if mixed with water. Usually the lipids will for ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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