Biological Expression Language Overview
... tloc(a(CHEBI:"sodium(1+)"), MESHCL:"Extracellular Space", \ ...
... tloc(a(CHEBI:"sodium(1+)"), MESHCL:"Extracellular Space", \ ...
as a PDF
... shift to -68 kDa in sodium dodecyl sulfate gels, which is very similar to that observed in Alzheimer-T . Tryptic digestion of 32 P-labelled T, followed by HPLC and twodimensional separation on TLC cellulose plates, revealed eight major phosphopeptides. Phosphoamino acid analysis together with sequen ...
... shift to -68 kDa in sodium dodecyl sulfate gels, which is very similar to that observed in Alzheimer-T . Tryptic digestion of 32 P-labelled T, followed by HPLC and twodimensional separation on TLC cellulose plates, revealed eight major phosphopeptides. Phosphoamino acid analysis together with sequen ...
CENTRAL TEXAS COLLEGE
... Describe the two types of secondary protein structure. Explain the role of hydrogen bonds in maintaining the structure. Explain how weak interactions and disulfide bridges contribute to tertiary protein structure. Using collagen and hemoglobin as examples, describe quaternary protein structure. Defi ...
... Describe the two types of secondary protein structure. Explain the role of hydrogen bonds in maintaining the structure. Explain how weak interactions and disulfide bridges contribute to tertiary protein structure. Using collagen and hemoglobin as examples, describe quaternary protein structure. Defi ...
DOCTORAL THESIS
... transglycosylase activity catalyzing cleavage and reconection of xyloglucan molecules, which is proposed to be involved in secondary cell wall morphogenesis. The creation of a family 16 carbohydrate active enzyme β-glucanase/XET hybrids were attempted in order to design a chimeric enzyme with one or ...
... transglycosylase activity catalyzing cleavage and reconection of xyloglucan molecules, which is proposed to be involved in secondary cell wall morphogenesis. The creation of a family 16 carbohydrate active enzyme β-glucanase/XET hybrids were attempted in order to design a chimeric enzyme with one or ...
Characters of Chymosin Gene Isolated from Different Animal A. G. Attallah
... for camel, pig, buffalo and cow respectively and it was corresponding with the sequence obtained from genomic analysis of pre-prochymosin gene [1 4 ]. Fig. (3) shows that PCR profiles of each primer we found that two main bands of approximately 1.2 kb and 0.25 kb for buffalo chymosin, 1.1 kb and 0.2 ...
... for camel, pig, buffalo and cow respectively and it was corresponding with the sequence obtained from genomic analysis of pre-prochymosin gene [1 4 ]. Fig. (3) shows that PCR profiles of each primer we found that two main bands of approximately 1.2 kb and 0.25 kb for buffalo chymosin, 1.1 kb and 0.2 ...
Ana Maria da Silva Esteves Dissertation presented to obtain
... membranar DIPPS domain was deemed highly relevant and challenging. The structure of the recombinant bifunctional protein IPCT/DIPPS of Archaeoglobus fulgidus with and without bound CDP-inositol was solved by X-ray crystallography to a resolution of 2.5 and 2.65 Å, respectively. The DIPPS domain comp ...
... membranar DIPPS domain was deemed highly relevant and challenging. The structure of the recombinant bifunctional protein IPCT/DIPPS of Archaeoglobus fulgidus with and without bound CDP-inositol was solved by X-ray crystallography to a resolution of 2.5 and 2.65 Å, respectively. The DIPPS domain comp ...
Hydrolysis of a Series of Synthetic Peptide Substrates by the Human
... for antiviral chemotherapy. By analogy, this is almost certainly true for the 3C proteins of all picornaviruses, including human rhinoviruses. However there have been relatively few comprehensive studies on the rhinovirus 3C protein (Libby et al., 1988). To ascertain not only whether this protein ex ...
... for antiviral chemotherapy. By analogy, this is almost certainly true for the 3C proteins of all picornaviruses, including human rhinoviruses. However there have been relatively few comprehensive studies on the rhinovirus 3C protein (Libby et al., 1988). To ascertain not only whether this protein ex ...
An investigation of the zinc binding characteristics of the RING finger
... RBBP6 contains a RING finger domain, which is a hallmark of E3 ubiquitin ligases, and which is essential for the interaction with Hdm2 (Li et al., 2007). RING fingers typically bind two Zn2+ ions, coordinated by eight conserved cysteine or histidine residues, typically forming a C3HC4 motif, althoug ...
... RBBP6 contains a RING finger domain, which is a hallmark of E3 ubiquitin ligases, and which is essential for the interaction with Hdm2 (Li et al., 2007). RING fingers typically bind two Zn2+ ions, coordinated by eight conserved cysteine or histidine residues, typically forming a C3HC4 motif, althoug ...
Queuosine formation in eukaryotic tRNA occurs via a mitochondria
... Ф-x2-3-Ф-x2-3-Ф-x-Ф (Ф = L, I, V, F, M; x is any amino acid) but the region is rich in glutamate, aspartate and serine and occurs at the end of a putative α-helix at the border of secondary structure elements; features typical of a leucine-rich export signal (28, 29). An insertion of 41 amino acids ...
... Ф-x2-3-Ф-x2-3-Ф-x-Ф (Ф = L, I, V, F, M; x is any amino acid) but the region is rich in glutamate, aspartate and serine and occurs at the end of a putative α-helix at the border of secondary structure elements; features typical of a leucine-rich export signal (28, 29). An insertion of 41 amino acids ...
Bio 226: Cell and Molecular Biology
... "Escape time" until a response can no longer be reversed by FR also varies: time taken for Pfr to do its job Conclusions: phytochrome acts on many processes in ...
... "Escape time" until a response can no longer be reversed by FR also varies: time taken for Pfr to do its job Conclusions: phytochrome acts on many processes in ...
Supplementary materials - Stanford Artificial Intelligence Laboratory
... Importantly, the candidate genetic regulators for Geronemo consist of both continuousvalued e-regulators and discrete-valued g-regulators. The continuous-valued candidate regulators have more possible split values than the discrete-valued ones; therefore it is possible that e-regulators can be captu ...
... Importantly, the candidate genetic regulators for Geronemo consist of both continuousvalued e-regulators and discrete-valued g-regulators. The continuous-valued candidate regulators have more possible split values than the discrete-valued ones; therefore it is possible that e-regulators can be captu ...
Biomarker discovery for psychiatric disorders
... 2007; Rosen and Schulkin, 1998). In its pathological form, anxiety encompasses a wide spectrum of conditions, including panic disorder, agoraphobia, social phobia, obsessive-compulsive disorder, generalized anxiety disorder, post-traumatic stress disorder and acute stress disorder (American Psychiat ...
... 2007; Rosen and Schulkin, 1998). In its pathological form, anxiety encompasses a wide spectrum of conditions, including panic disorder, agoraphobia, social phobia, obsessive-compulsive disorder, generalized anxiety disorder, post-traumatic stress disorder and acute stress disorder (American Psychiat ...
SensiFAST™ Two-Step Real-Time RT-PCR
... such as in high-throughput detection of a specific target ...
... such as in high-throughput detection of a specific target ...
A conserved N-‐terminal domain of Rif2 regulates telomere length in
... The regulation of telomere length equilibrium is essential for cell growth and survival since critically short telomeres signal DNA damage and cell cycle arrest. While the broad principles of length regulatio ...
... The regulation of telomere length equilibrium is essential for cell growth and survival since critically short telomeres signal DNA damage and cell cycle arrest. While the broad principles of length regulatio ...
Abiotic stress in plants: Late Embryogenesis Abundant proteins Imen Amara
... and Shinozaki 2006). In Arabidopsis gene networks of stress-inducible genes have been identified and have been classified into two groups: regulatory and functional genes (Cramer et al. 2011; Shinozaki and Yamaguchi-Shinozaki 2007) (Figure 1). The first group comprises regulatory proteins, i.e. prot ...
... and Shinozaki 2006). In Arabidopsis gene networks of stress-inducible genes have been identified and have been classified into two groups: regulatory and functional genes (Cramer et al. 2011; Shinozaki and Yamaguchi-Shinozaki 2007) (Figure 1). The first group comprises regulatory proteins, i.e. prot ...
Rabbit genome editing with zinc finger nucleases
... nology may be used to edit a particular gene in a potential model organism in order to develop an animal model of a certain human or rabbit disease. HoWever, gene knockout technology may require months or years to construct and ...
... nology may be used to edit a particular gene in a potential model organism in order to develop an animal model of a certain human or rabbit disease. HoWever, gene knockout technology may require months or years to construct and ...
Cra-mediated regulation of Escherichia coli adenylate cyclase
... of many genes (Saier & Ramseier, 1996). cAMP is also essential for transcriptional regulation of many genes via its receptor protein CRP. When complexed with CAMP, CRP binds to specific sites upstream of promoters, causing transcriptional activation or repression (Kolb et al., 1993). A CRP binding s ...
... of many genes (Saier & Ramseier, 1996). cAMP is also essential for transcriptional regulation of many genes via its receptor protein CRP. When complexed with CAMP, CRP binds to specific sites upstream of promoters, causing transcriptional activation or repression (Kolb et al., 1993). A CRP binding s ...
A chitin-binding domain in a marine bacterial
... sequencing the clones outlined in Fig. 1. The ORF of chiA encodes a 729 residue protein with a calculated molecular mass of 92 kDa. Analysis of the predicted amino acid sequence revealed characteristic features of a signal peptide which is required for membrane translocation (Inouye & Halegoua, 1980 ...
... sequencing the clones outlined in Fig. 1. The ORF of chiA encodes a 729 residue protein with a calculated molecular mass of 92 kDa. Analysis of the predicted amino acid sequence revealed characteristic features of a signal peptide which is required for membrane translocation (Inouye & Halegoua, 1980 ...
review on enhancement of glucose uptake and up
... CE and CP with procyanidin type-A polymers exhibit the potential to increase the amount of protein tristetraprolin (TTP), IR, and GLUT4 in mouse 3T3-L1 adipocytes. Immunoblotting showed that CP increased IR levels and that both CE and CP increased GLUT4 and TTP levels in the adipocytes. Quantitative ...
... CE and CP with procyanidin type-A polymers exhibit the potential to increase the amount of protein tristetraprolin (TTP), IR, and GLUT4 in mouse 3T3-L1 adipocytes. Immunoblotting showed that CP increased IR levels and that both CE and CP increased GLUT4 and TTP levels in the adipocytes. Quantitative ...
Large-scale Analysis of Pseudogenes in the Human Genome
... Mammalian genomes, such as human and mouse, contain large number of gene-like sequences called pseudogenes. These pseudogenes are inheritable, non-functional, gene homologies that are generally disabled at transcriptional level [1,2]. In most cases, pseudogenes cannot produce transcripts due to the ...
... Mammalian genomes, such as human and mouse, contain large number of gene-like sequences called pseudogenes. These pseudogenes are inheritable, non-functional, gene homologies that are generally disabled at transcriptional level [1,2]. In most cases, pseudogenes cannot produce transcripts due to the ...
unexpected consequences for sense codon reassignment
... acid with the ncAA. Residue specific reassignment operates through precisely controlling the growth medium such that the targeted natural amino acid is removed and replaced by a ncAA that is a close structural analog (24-27). The ncAA is utilized in translation in place of the removed natural amino ...
... acid with the ncAA. Residue specific reassignment operates through precisely controlling the growth medium such that the targeted natural amino acid is removed and replaced by a ncAA that is a close structural analog (24-27). The ncAA is utilized in translation in place of the removed natural amino ...
Biological significance of structural differences between two highly
... (Fig. 1A), because it lies in proximity to a.a. 116–129 in the Uev1 molecular structure (Fig. 1C). The Uev1D30-F104S single amino acid substitution abrogated binding by LN2A, but did not affect ...
... (Fig. 1A), because it lies in proximity to a.a. 116–129 in the Uev1 molecular structure (Fig. 1C). The Uev1D30-F104S single amino acid substitution abrogated binding by LN2A, but did not affect ...
Document
... NMR-visible component display characteristic chemical shift changes upon increasing amounts of the additionally expressed, unlabeled ligand [24,25]. This effect can be employed to comparatively analyze in vitro and in vivo binding behaviors of known interaction partners, or to test how small molec ...
... NMR-visible component display characteristic chemical shift changes upon increasing amounts of the additionally expressed, unlabeled ligand [24,25]. This effect can be employed to comparatively analyze in vitro and in vivo binding behaviors of known interaction partners, or to test how small molec ...
Characterization of the chimeric seven
... wild-type BPR (491 nm) at pH 7. The absorption maxima of all the CFR isolates were between 515 and 522 nm at pH 7 (Table 2), which falls into the green light region. CFR 1 absorbs light maximally at 545 and 513, at pH 4 and 10, respectively (Fig. 4). The most blue-shifted CFR variant, CFR3, displaye ...
... wild-type BPR (491 nm) at pH 7. The absorption maxima of all the CFR isolates were between 515 and 522 nm at pH 7 (Table 2), which falls into the green light region. CFR 1 absorbs light maximally at 545 and 513, at pH 4 and 10, respectively (Fig. 4). The most blue-shifted CFR variant, CFR3, displaye ...
Enzymatic activation of sulfur for incorporation into biomolecules in
... although via more indirect pathways (see below). The central importance of IscS in E. coli has motivated innumerable searches for sequence homologs in other organisms and often iscS-type genes can be identified by the encoded IscS protein signature motifs and the neighborhood to other isc-type compo ...
... although via more indirect pathways (see below). The central importance of IscS in E. coli has motivated innumerable searches for sequence homologs in other organisms and often iscS-type genes can be identified by the encoded IscS protein signature motifs and the neighborhood to other isc-type compo ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.