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Comparison of Protein Active Site Structures for
... complexed to a ligand were excluded, since they lack any value in the functional annotation of a protein. The identification of a ligand within a protein PDB file was determined by the presence of either a HET or HETNAM record. Routinely, a single protein PDB file may contain multiple ligands. Each ...
... complexed to a ligand were excluded, since they lack any value in the functional annotation of a protein. The identification of a ligand within a protein PDB file was determined by the presence of either a HET or HETNAM record. Routinely, a single protein PDB file may contain multiple ligands. Each ...
Sample pages 1 PDF
... (Fig. 2.4). In bacteria, the 30S subunit directly binds mRNA in the vicinity of the start codon. This process is mediated by the Shine–Dalgarno sequence, a unique feature of bacterial mRNAs that is located upstream of the start codon (Shine and Dalgarno 1974). This sequence interacts with a compleme ...
... (Fig. 2.4). In bacteria, the 30S subunit directly binds mRNA in the vicinity of the start codon. This process is mediated by the Shine–Dalgarno sequence, a unique feature of bacterial mRNAs that is located upstream of the start codon (Shine and Dalgarno 1974). This sequence interacts with a compleme ...
OsCUL3a Negatively Regulates Cell Death and
... formation (cell death) and disease resistance in rice. OsCUL3a Interacts with Both OsRBX1a and OsRBX1b in Vivo Cullin proteins can assemble with RBX and various adaptors to form CRLs in eukaryotes (Vierstra, 2009). Rice has two genes that encode RBX-like proteins, OsRBX1a and OsRBX1b, which share 92 ...
... formation (cell death) and disease resistance in rice. OsCUL3a Interacts with Both OsRBX1a and OsRBX1b in Vivo Cullin proteins can assemble with RBX and various adaptors to form CRLs in eukaryotes (Vierstra, 2009). Rice has two genes that encode RBX-like proteins, OsRBX1a and OsRBX1b, which share 92 ...
Gene Section DNMT1 (DNA (cytosine-5-)-methyltransferase 1)) Atlas of Genetics and Cytogenetics
... DNMT1 has different translational start points, and exists as different splice variants. The predominant splicing isoform in human somatic cells comprises. 1616 amino acid residues. A shorter germ-cell-specific form of DNMT1 known as DNMT1o is found in growing oocytes and during embryonic preimplant ...
... DNMT1 has different translational start points, and exists as different splice variants. The predominant splicing isoform in human somatic cells comprises. 1616 amino acid residues. A shorter germ-cell-specific form of DNMT1 known as DNMT1o is found in growing oocytes and during embryonic preimplant ...
in Graminaceous Plants
... sion vector for yeast, and has the ADH (alcohol dehydrogenase) promoter driving expression of the introduced cDNAs. The cDNA library was screened by colony hybridization using the PCR product (probe I, see Fig. 2). The probe was labeled with [a-32P]dATP using a randomprimer labeling kit (version 2, ...
... sion vector for yeast, and has the ADH (alcohol dehydrogenase) promoter driving expression of the introduced cDNAs. The cDNA library was screened by colony hybridization using the PCR product (probe I, see Fig. 2). The probe was labeled with [a-32P]dATP using a randomprimer labeling kit (version 2, ...
Rabbit Reticulocyte Lysate Technical Manual
... Rabbit Reticulocyte Lysate may contain a variety of post-translational processing activities, including acetylation, isoprenylation and some phosphorylation activity that will vary from lot to lot (2). Processing events such as signal peptide cleavage and core glycosylation can be examined by adding ...
... Rabbit Reticulocyte Lysate may contain a variety of post-translational processing activities, including acetylation, isoprenylation and some phosphorylation activity that will vary from lot to lot (2). Processing events such as signal peptide cleavage and core glycosylation can be examined by adding ...
Phosphate binding sites identification in protein
... The ability to bind phosphate has evolved in many non-homologous protein families. There are however some preeminent groups that dominate this distribution such as that of P-loop containing proteins (5) or proteins with a Rossmann-type fold (6). The possibility to characterize a protein for its abil ...
... The ability to bind phosphate has evolved in many non-homologous protein families. There are however some preeminent groups that dominate this distribution such as that of P-loop containing proteins (5) or proteins with a Rossmann-type fold (6). The possibility to characterize a protein for its abil ...
Bioinformatics of proteins: Sequence, structure and the `symbiosis
... From Sequence to Structure ...
... From Sequence to Structure ...
Expressing the multifunctional nucleoside kinase of : Drosophila melanogaster Shuba Krishnan
... 5.3 Analysis of Dm-dNK Protein Expression......................................................................... 7 5.5 Quantification of mtDNA by Real-time PCR (RT-PCR) ................................................. 9 6. Discussion ............................................................... ...
... 5.3 Analysis of Dm-dNK Protein Expression......................................................................... 7 5.5 Quantification of mtDNA by Real-time PCR (RT-PCR) ................................................. 9 6. Discussion ............................................................... ...
The Plant Cell
... was identified from the GenBank database. These proteins contained predicted coiled-coil and Src homology 3 (SH3) domains that are similar to animal and yeast proteins involved in the formation, fission, and uncoating of clathrin-coated vesicles. Subcellular fractionation and immunolocalization stud ...
... was identified from the GenBank database. These proteins contained predicted coiled-coil and Src homology 3 (SH3) domains that are similar to animal and yeast proteins involved in the formation, fission, and uncoating of clathrin-coated vesicles. Subcellular fractionation and immunolocalization stud ...
Expression of phosphofructokinase in Neisseria meningitidis
... phosphate pathway. The Embden–Meyerhof–Parnas pathway is not functional, because the gene for phosphofructokinase (PFK) is not present. The phylogenetic distribution of PFK indicates that in most obligate aerobic organisms, PFK is lacking. We conclude that this is because of the limited contribution ...
... phosphate pathway. The Embden–Meyerhof–Parnas pathway is not functional, because the gene for phosphofructokinase (PFK) is not present. The phylogenetic distribution of PFK indicates that in most obligate aerobic organisms, PFK is lacking. We conclude that this is because of the limited contribution ...
Structure-Based Prediction of DNA Target Sites by Regulatory Proteins
... These results show that target binding sites for several regulatory proteins are successfully predicted, and our data suggest that this method can serve as a powerful tool for predicting multiple target sites and target genes for regulatory proteins. Proteins 1999;35:114–131. r 1999 Wiley-Liss, Inc. ...
... These results show that target binding sites for several regulatory proteins are successfully predicted, and our data suggest that this method can serve as a powerful tool for predicting multiple target sites and target genes for regulatory proteins. Proteins 1999;35:114–131. r 1999 Wiley-Liss, Inc. ...
Chapter 9 Proteins - Angelo State University
... • Peptides are named by starting at the N-terminal end and listing the amino acid residues from left to right. • Large amino acid chains are unwieldy to draw in their complete forms, so they are usually represented by their three-letter abbreviations, separated by dashes: – Gly-Ala (Gly = N-terminal ...
... • Peptides are named by starting at the N-terminal end and listing the amino acid residues from left to right. • Large amino acid chains are unwieldy to draw in their complete forms, so they are usually represented by their three-letter abbreviations, separated by dashes: – Gly-Ala (Gly = N-terminal ...
The Wiskott-Aldrich Syndrome and X-Linked
... platelets,eczema,recurrentinfections,andimmunodeficiency. Besidesthe classic WAS phenotype,there is a group of patients with congenitalX-linkedthrombocytopenia (XLT) who have small platelets but only transient eczema, if any, and minimal immune deficiency. Because the gene responsible for WAS has be ...
... platelets,eczema,recurrentinfections,andimmunodeficiency. Besidesthe classic WAS phenotype,there is a group of patients with congenitalX-linkedthrombocytopenia (XLT) who have small platelets but only transient eczema, if any, and minimal immune deficiency. Because the gene responsible for WAS has be ...
Module Document
... southern species (warm-adapted) increased significantly over northern species (coldadapted), during a time period where ocean and summer air temperatures had both increased over the 60-year span of time. What this study suggests is that such a change can eliminate some species from the community—per ...
... southern species (warm-adapted) increased significantly over northern species (coldadapted), during a time period where ocean and summer air temperatures had both increased over the 60-year span of time. What this study suggests is that such a change can eliminate some species from the community—per ...
The initiation phase of protein synthesis in eukaryotes
... Figure 7 The binding of the 4E-BPs to eIF4E is regulated by phosphorylation. The 4E-BPs and eIF4Gs compete for a common binding site on eIF4E. Various stimuli increase the phosphorylation of the 4E-BPs. Hyperphosphorylated 4E-BPs have a relatively low affinity for eIF4E. Conversely, a decrease in 4E ...
... Figure 7 The binding of the 4E-BPs to eIF4E is regulated by phosphorylation. The 4E-BPs and eIF4Gs compete for a common binding site on eIF4E. Various stimuli increase the phosphorylation of the 4E-BPs. Hyperphosphorylated 4E-BPs have a relatively low affinity for eIF4E. Conversely, a decrease in 4E ...
The Genetic Code and RNA-Amino Acid Affinities
... slowly release a ligand selects most stable binding, for example, by L-Arg [22]. Alternatively, if a selection allows RNAs to compete for reaction at a limited number of sites, selection of the fastest reacting can be the result [23,24]. However, in the absence of such functional pressures, the most ...
... slowly release a ligand selects most stable binding, for example, by L-Arg [22]. Alternatively, if a selection allows RNAs to compete for reaction at a limited number of sites, selection of the fastest reacting can be the result [23,24]. However, in the absence of such functional pressures, the most ...
Article Lateral Gene Transfer and Gene
... mitochondria, and phylogenetic analysis of its two key components, ATP sulfurylase (AS) and adenosine-50 -phosphosulfate kinase (APSK), suggests that the corresponding genes were acquired laterally from a bacterial source. Interestingly, in Entamoeba’s relative M. balamuthi, PFO and hydrogenase are ...
... mitochondria, and phylogenetic analysis of its two key components, ATP sulfurylase (AS) and adenosine-50 -phosphosulfate kinase (APSK), suggests that the corresponding genes were acquired laterally from a bacterial source. Interestingly, in Entamoeba’s relative M. balamuthi, PFO and hydrogenase are ...
Tumor cell metabolism: the marriage of molecular genetics and
... are then extrapolated to cellular metabolic events. The weakness of this group is the absence of training in and understanding of the principles of enzymology, the factors that effect cellular enzyme activity, and the relationships of sequential enzyme activities in metabolic pathways. This is the e ...
... are then extrapolated to cellular metabolic events. The weakness of this group is the absence of training in and understanding of the principles of enzymology, the factors that effect cellular enzyme activity, and the relationships of sequential enzyme activities in metabolic pathways. This is the e ...
DNA replication - U of L Class Index
... They are the binding sites for the Tus proteins (terminus utilization substance). Sequences must be disentangled. ...
... They are the binding sites for the Tus proteins (terminus utilization substance). Sequences must be disentangled. ...
Histone Modifications - Life Science Saga
... of gene either transcriptionally active or inactive. ...
... of gene either transcriptionally active or inactive. ...
ppt - University of Connecticut
... Exon - a region of a gene which is translated into protein Intron - a region of a gene which is not translated into protein Splicing – a process in which the introns are removed and exons are joined to be translated into a single protein ...
... Exon - a region of a gene which is translated into protein Intron - a region of a gene which is not translated into protein Splicing – a process in which the introns are removed and exons are joined to be translated into a single protein ...
Purine Biosynthesis. Big in Cell Division, Even
... Figure 3. Schematic diagram showing the structural organization of enzymes of the de novo purine biosynthesis pathway from a range of organisms. In E. coli, each enzyme is monofunctional, except aminoimidazolecarboximide ribonucleotide transformylase/inosine monophosphate cyclohydrolase, which is bi ...
... Figure 3. Schematic diagram showing the structural organization of enzymes of the de novo purine biosynthesis pathway from a range of organisms. In E. coli, each enzyme is monofunctional, except aminoimidazolecarboximide ribonucleotide transformylase/inosine monophosphate cyclohydrolase, which is bi ...
Bioinorganic motifs: towards functional classification of metalloproteins
... to the concept of bioinorganic motif. The definition for terms given in italic are summarized in the Glossary. The Glossary of Terms in Bioinorganic Chemistry (de Bolster, 1997) contains definitions for approximately 400 terms of relevance and I recommend it for further reference. The same low-molec ...
... to the concept of bioinorganic motif. The definition for terms given in italic are summarized in the Glossary. The Glossary of Terms in Bioinorganic Chemistry (de Bolster, 1997) contains definitions for approximately 400 terms of relevance and I recommend it for further reference. The same low-molec ...
UvA-DARE (Digital Academic Repository) The role of yeast NAD+
... Mitochondriall mRNAs are uncapped and predominantly possess extremely long untranslatedd leader and trailer sequences of high A+U content (>95%). These leader sequencess are likely to be difficult to scan for a ribosome, since they often contain shortt open reading frames and short G+C rich clusters ...
... Mitochondriall mRNAs are uncapped and predominantly possess extremely long untranslatedd leader and trailer sequences of high A+U content (>95%). These leader sequencess are likely to be difficult to scan for a ribosome, since they often contain shortt open reading frames and short G+C rich clusters ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.