![Computer Storage of Sequences](http://s1.studyres.com/store/data/022275328_1-5956ca41a91c45fa7610aa7057b77fc6-300x300.png)
Computer Storage of Sequences
... (Non-redundant REFerence protein database) Comprehensive: Containing all sequences from PIR-PSD, SwissProt, TrEMBL, RefSeq, GenPept, and updated bi-weekly. Non-Redundant: Clustered by sequence identity and taxonomy at the species level. ...
... (Non-redundant REFerence protein database) Comprehensive: Containing all sequences from PIR-PSD, SwissProt, TrEMBL, RefSeq, GenPept, and updated bi-weekly. Non-Redundant: Clustered by sequence identity and taxonomy at the species level. ...
attachment 2 - Food Standards Australia New Zealand
... of T-DNA containing three expression cassettes for the genes 2m epsps, aad-12 and pat has been inserted at a single locus in Chromosome 6 of the soybean genome. No DNA sequences from the backbone of the transformation vector, including antibiotic resistance marker genes, were transferred to the plan ...
... of T-DNA containing three expression cassettes for the genes 2m epsps, aad-12 and pat has been inserted at a single locus in Chromosome 6 of the soybean genome. No DNA sequences from the backbone of the transformation vector, including antibiotic resistance marker genes, were transferred to the plan ...
Lab Session 9
... What if not using a stacking gel? • In the absence of a stacking gel, your sample would sit on top of the running gel, as a band of up to 1cm deep. • Rather than being lined up together and hitting the running gel together, the proteins in the sample would all enter the running gel at different tim ...
... What if not using a stacking gel? • In the absence of a stacking gel, your sample would sit on top of the running gel, as a band of up to 1cm deep. • Rather than being lined up together and hitting the running gel together, the proteins in the sample would all enter the running gel at different tim ...
Putrescine oxidase of Micrococcus rubens : primary
... ligated mixture was then introduced by transformation into E. coli JM 109 and ampicillin-resistant transformants were selected on 2 x YT medium containing 50 pg ampicillin ml-'. Colony hybridization (Grunstein & Hogness, 1975) with the 32P-labelledprobe designed for the NH,-terminal amino acid seque ...
... ligated mixture was then introduced by transformation into E. coli JM 109 and ampicillin-resistant transformants were selected on 2 x YT medium containing 50 pg ampicillin ml-'. Colony hybridization (Grunstein & Hogness, 1975) with the 32P-labelledprobe designed for the NH,-terminal amino acid seque ...
Phylogenetic relationship of phototrophic purple sulfur bacteria
... can be easily distinguished by their ability to store elemental sulfur inside or outside the cells as an intermediate during photosynthesis. Functional gene approaches can support phylogenetic considerations concluded from 16S rRNA gene analysis. This has already been demonstrated for the FennaMatht ...
... can be easily distinguished by their ability to store elemental sulfur inside or outside the cells as an intermediate during photosynthesis. Functional gene approaches can support phylogenetic considerations concluded from 16S rRNA gene analysis. This has already been demonstrated for the FennaMatht ...
Which Protein is Best?
... Protein has long been associated with increased satiety, thereby improving weight loss and promoting adherence to difficult, otherwise hunger-inducing diets. While protein may keep you fuller longer, a new study, due to appear in the July issue of The Journal of Nutrition, showed that whey protein is ...
... Protein has long been associated with increased satiety, thereby improving weight loss and promoting adherence to difficult, otherwise hunger-inducing diets. While protein may keep you fuller longer, a new study, due to appear in the July issue of The Journal of Nutrition, showed that whey protein is ...
THE IDENTIFICATION AND CHARACTERISATION OF THE
... adenylation domain may be used for the recognition and activation of phenylalanine as a starting molecule in the biosynthesis of DPO. However, it is possible that clones A-18 and A-66 have substrate specificities for amino acids other than phenylalanine and this is due to the fact that the NRPSpredi ...
... adenylation domain may be used for the recognition and activation of phenylalanine as a starting molecule in the biosynthesis of DPO. However, it is possible that clones A-18 and A-66 have substrate specificities for amino acids other than phenylalanine and this is due to the fact that the NRPSpredi ...
Cloning and characterization of the
... deletion of either LOS1 or PUS1 does not result in a growth defect for yeast cells, whereas combined disruption of both genes causes slow cell growth at 30°C as well as a thermosensitive phenotype, i.e. lack of viability at 37°C (4). This genetic interaction suggests that pseudouridinylation of tRNA ...
... deletion of either LOS1 or PUS1 does not result in a growth defect for yeast cells, whereas combined disruption of both genes causes slow cell growth at 30°C as well as a thermosensitive phenotype, i.e. lack of viability at 37°C (4). This genetic interaction suggests that pseudouridinylation of tRNA ...
Translation of Cyclin mRNA Is Necessary for Extracts of Activated
... of inducing amphibian oocytes to enter meiosis. It has subsequently been identified in a wide variety of meiotic and mitotic cells from yeast to man (Kishimoto and Kanatani, 1976; Wasserman and Smith, 1978; Sunkara et al., 1979; Kishimoto et al., 1982; Weintraub et al., 1982; Gerhart et al., 1984; M ...
... of inducing amphibian oocytes to enter meiosis. It has subsequently been identified in a wide variety of meiotic and mitotic cells from yeast to man (Kishimoto and Kanatani, 1976; Wasserman and Smith, 1978; Sunkara et al., 1979; Kishimoto et al., 1982; Weintraub et al., 1982; Gerhart et al., 1984; M ...
A novel sensitive method for the detection of user
... not only in sequence similarity searches. DNA sequences contain a large fraction of diverse biased segments with poorly understood function. More than 50% of all proteins have been shown to contain biased segments (Wootton and Federhen, 1996). Some researchers believe that they are similar to junk D ...
... not only in sequence similarity searches. DNA sequences contain a large fraction of diverse biased segments with poorly understood function. More than 50% of all proteins have been shown to contain biased segments (Wootton and Federhen, 1996). Some researchers believe that they are similar to junk D ...
Hydrodynamic Studies on the Manganese
... the isolation of this protein were performed at 4 °C. Chloroplasts were isolated as previously described (15). PS II membranes were isolated by the procedure of Berthold et al. (16) as modified by Ghanotakis and Babcock (17). These membranes were suspended to a concentration of 1 mg/mL chl in 1.0 M ...
... the isolation of this protein were performed at 4 °C. Chloroplasts were isolated as previously described (15). PS II membranes were isolated by the procedure of Berthold et al. (16) as modified by Ghanotakis and Babcock (17). These membranes were suspended to a concentration of 1 mg/mL chl in 1.0 M ...
Section 3: Prokaryotic Sample and Array Processing
... Total RNA Isolation As starting material for the cDNA synthesis procedure, total RNA can be isolated by using standard procedures for bacterial RNA isolation or various commercial RNA isolation kits. For Pseudomonas aeruginosa and E. coli, we have successfully used the QIAGEN® RNeasy Mini Purificati ...
... Total RNA Isolation As starting material for the cDNA synthesis procedure, total RNA can be isolated by using standard procedures for bacterial RNA isolation or various commercial RNA isolation kits. For Pseudomonas aeruginosa and E. coli, we have successfully used the QIAGEN® RNeasy Mini Purificati ...
16_Lecture_Presentation
... – Cistron: Part of nucleotide sequence coding for single gene – Single mRNA is translated into three gene products ...
... – Cistron: Part of nucleotide sequence coding for single gene – Single mRNA is translated into three gene products ...
Large-scale analysis of Influenza A virus nucleoprotein sequence
... 1. Conservation grades were mapped to the protein backbone (figure 1A, B). Overall the protein is highly conserved with 59% of residues in the highest conservation grades 7-9, while 21% of residues are highly variable with grades 1-3. A significant number of 38 residues highlighted in table 1 show n ...
... 1. Conservation grades were mapped to the protein backbone (figure 1A, B). Overall the protein is highly conserved with 59% of residues in the highest conservation grades 7-9, while 21% of residues are highly variable with grades 1-3. A significant number of 38 residues highlighted in table 1 show n ...
Protein aggregation and amyloid fibril formation prediction software
... database) are used as a prototype scale for constructing a packing density profile for a certain protein sequence. Calculations are based on the sliding-window averaging technique. First, an expected value is assigned to each residue of the protein, equal to the average packing density value observed ...
... database) are used as a prototype scale for constructing a packing density profile for a certain protein sequence. Calculations are based on the sliding-window averaging technique. First, an expected value is assigned to each residue of the protein, equal to the average packing density value observed ...
Recognition Specificity for the Bacterial Avirulence Protein AvrPto Is
... recognition is further supported by analysis of the Pto kinase encoded by an allele from a bacterial speck susceptible tomato plant (Jia et al., 1997). This kinase, which does not interact with AvrPto in the two-hybrid assay, contains a phenylalanine at position L205 of Pto and contains a small dele ...
... recognition is further supported by analysis of the Pto kinase encoded by an allele from a bacterial speck susceptible tomato plant (Jia et al., 1997). This kinase, which does not interact with AvrPto in the two-hybrid assay, contains a phenylalanine at position L205 of Pto and contains a small dele ...
Nomenclature for incompletely specified bases in nucleic acid
... 'NNNNNCNNGNTNN'with'-----C--G-T--, for example. Note that the use of the lower-case letter n may avoid the necessity for an additional symbol, as in 'nnnnnCnnGnTnn'. In addition, the use of the oblique or slash / may present advantages in the definition of the precise cleavage sites of restriction e ...
... 'NNNNNCNNGNTNN'with'-----C--G-T--, for example. Note that the use of the lower-case letter n may avoid the necessity for an additional symbol, as in 'nnnnnCnnGnTnn'. In addition, the use of the oblique or slash / may present advantages in the definition of the precise cleavage sites of restriction e ...
Characterization of Complementary DNA Encoding the Precursor for
... identified two forms of GnRH in several teleost species, including salmon, trout, goldfish, herring, mullet, siganids, milkfish, and sea bass (2). However, the exact amino acid sequence of only one form, salmon I, is known (15). This peptide sequence was used to design the oligonucleotides that succ ...
... identified two forms of GnRH in several teleost species, including salmon, trout, goldfish, herring, mullet, siganids, milkfish, and sea bass (2). However, the exact amino acid sequence of only one form, salmon I, is known (15). This peptide sequence was used to design the oligonucleotides that succ ...
Complementary DNA Cloning, Messenger RNA
... nantly involved in detoxification reactions (1,2). They are found in all tissues and species, including bacteria (3), yeast (4), and plants (2). In higher organisms, three classes of GST protein, a, n, and 7T(1, 2), have been discriminated; a fourth class, 0, has been tentatively identified, althoug ...
... nantly involved in detoxification reactions (1,2). They are found in all tissues and species, including bacteria (3), yeast (4), and plants (2). In higher organisms, three classes of GST protein, a, n, and 7T(1, 2), have been discriminated; a fourth class, 0, has been tentatively identified, althoug ...
Electromagnetic Properties of Biomolecules
... interactions between proteins and their targets (other proteins, DNA regulatory segments or small molecules). Each of these processes involves an energy transfer between the interacting molecules. These interactions are highly selective, and this selectivity is defined within the protein primary str ...
... interactions between proteins and their targets (other proteins, DNA regulatory segments or small molecules). Each of these processes involves an energy transfer between the interacting molecules. These interactions are highly selective, and this selectivity is defined within the protein primary str ...
Atom depth in protein structure and function
... several molecular, residue and atomic properties, such as average protein domain size, protein stability, free energy of formation of protein complexes, amino acid type hydrophobicity, residue conservation and hydrogen/deuterium amide proton exchange rates. Although the functional properties of a pr ...
... several molecular, residue and atomic properties, such as average protein domain size, protein stability, free energy of formation of protein complexes, amino acid type hydrophobicity, residue conservation and hydrogen/deuterium amide proton exchange rates. Although the functional properties of a pr ...
The Crystal Structure of Arabidopsis thaliana Allene Oxide Cyclase
... retinol binding protein (RBP; 1AQB) and nitrophorin (1NP1) each with a Z-score of 3.2. The lipocalin protein family (Pervaiz and Brew, 1985, 1987; Grzyb et al., 2006) is a large group of small extracellular proteins with great diversity at the sequence level but mostly with either three characterist ...
... retinol binding protein (RBP; 1AQB) and nitrophorin (1NP1) each with a Z-score of 3.2. The lipocalin protein family (Pervaiz and Brew, 1985, 1987; Grzyb et al., 2006) is a large group of small extracellular proteins with great diversity at the sequence level but mostly with either three characterist ...
Consistent Errors in First Strand cDNA Due to Random Hexamer
... We analyzed two independently generated RNA-seq datasets, focusing on reads mapping to ERCC spikes which are artificial RNA fragments of known sequence that are added during library preparation[4]. Reverse mapping reads represent first strand cDNA and forward mapping reads represent second strand cD ...
... We analyzed two independently generated RNA-seq datasets, focusing on reads mapping to ERCC spikes which are artificial RNA fragments of known sequence that are added during library preparation[4]. Reverse mapping reads represent first strand cDNA and forward mapping reads represent second strand cD ...
Intrinsically Disordered Domains of the B Cell Receptor
... nearly 30% of the proteome is comprised of MPs, only 1% of all deposited structures in the protein data bank (PDB) are MPs. This is largely due to the inherent difficulties associated with working with this class of proteins [3]. Cell membranes are permeable barriers that maintain and protect the in ...
... nearly 30% of the proteome is comprised of MPs, only 1% of all deposited structures in the protein data bank (PDB) are MPs. This is largely due to the inherent difficulties associated with working with this class of proteins [3]. Cell membranes are permeable barriers that maintain and protect the in ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.