Evaluation of existing motif detection tools on their
... - Putative direct regulation by VDR - VDRE hard to discover de novo: only one conserved half-site! •Two conserved half sites with variable spacer ...
... - Putative direct regulation by VDR - VDRE hard to discover de novo: only one conserved half-site! •Two conserved half sites with variable spacer ...
9 Proteins Chapter 9 Proteins
... • These structures result from four types of interactions between the R side chains of the amino acids residues: 1. Disulfide bridges can form between two cysteine residues that are close to each other in the same chain, or between cysteine residues in different chains. These bridges hold the protei ...
... • These structures result from four types of interactions between the R side chains of the amino acids residues: 1. Disulfide bridges can form between two cysteine residues that are close to each other in the same chain, or between cysteine residues in different chains. These bridges hold the protei ...
Protein-only inheritance in yeast: something to get
... determinant is eliminated rather than masked10. The only known function of Hsp104 is to alter the physical states of other proteins34,35. That a heritable change in phenotype in yeast can be induced by the transient overexpression of Hsp104 provides compelling support for [PSI1] as a yeast prion. An ...
... determinant is eliminated rather than masked10. The only known function of Hsp104 is to alter the physical states of other proteins34,35. That a heritable change in phenotype in yeast can be induced by the transient overexpression of Hsp104 provides compelling support for [PSI1] as a yeast prion. An ...
Improving representation of biological responses
... Valerie Wood Varsha Khodiyar Stan Laulederkind ...
... Valerie Wood Varsha Khodiyar Stan Laulederkind ...
Probe design for microarrays using OligoWiz
... Another way of ensuring a optimal discrimination between target and non-target under hybridization is to design all the oligos on an array with similar affinity for their targets. This will allow the experimentalist to optimize the hybridization conditions for all oligos by choosing the right hybrid ...
... Another way of ensuring a optimal discrimination between target and non-target under hybridization is to design all the oligos on an array with similar affinity for their targets. This will allow the experimentalist to optimize the hybridization conditions for all oligos by choosing the right hybrid ...
High Density Cobalt Agarose
... from bulk affinity resins: • Shake the bottle manually to obtain a homogenous suspension of the affinity resin. Place a funnel in the column head and slowly pour the suspension down the walls of the column. Please note: Add the suspension slowly to avoid the formation of bubbles. The suspension may al ...
... from bulk affinity resins: • Shake the bottle manually to obtain a homogenous suspension of the affinity resin. Place a funnel in the column head and slowly pour the suspension down the walls of the column. Please note: Add the suspension slowly to avoid the formation of bubbles. The suspension may al ...
Transcriptional activity and role of plasmids of
... Department of Pathology and Laboratory Medicine, College of Medicine, University of Saskatchewan, Room 2841, Royal University Hospital, 103 Hospital Drive, Saskatoon, SK Canada S7N 0W8 Contango Strategies Ltd., 410 Downey Road, Saskatoon SK, Canada S7N 4N1 Corresponding author: 5B53 Health Sciences ...
... Department of Pathology and Laboratory Medicine, College of Medicine, University of Saskatchewan, Room 2841, Royal University Hospital, 103 Hospital Drive, Saskatoon, SK Canada S7N 0W8 Contango Strategies Ltd., 410 Downey Road, Saskatoon SK, Canada S7N 4N1 Corresponding author: 5B53 Health Sciences ...
Monte Carlo Simulations of HIV Capsid Protein
... virus, and disruption of the capsid by inhibitors could potentially serve to treat virus infections.2,3 The mature HIV capsid is formed by multiple copies of a single capsid protein (CA). The structure of CA is largely α-helical, with two relatively rigid domains, respectively termed the N-terminal ...
... virus, and disruption of the capsid by inhibitors could potentially serve to treat virus infections.2,3 The mature HIV capsid is formed by multiple copies of a single capsid protein (CA). The structure of CA is largely α-helical, with two relatively rigid domains, respectively termed the N-terminal ...
human-physiology-ii-lecture-endomembrane
... a positively charged N-terminal region; a central hydrophobic region; a polar region adjoining the site where cleavage from the mature protein will take place. A signal sequence on nascent seretory proteins targets them to the ER and is then cleaved off. SRP receptor (a binding protein or docking pr ...
... a positively charged N-terminal region; a central hydrophobic region; a polar region adjoining the site where cleavage from the mature protein will take place. A signal sequence on nascent seretory proteins targets them to the ER and is then cleaved off. SRP receptor (a binding protein or docking pr ...
Molecule of the Month: AgrA DNA Binding Domain AgrA is the
... DNA bases – N201 makes contact via the phosphate backbone. This is interesting because other nucleotides in the binding site are quite important for binding (and conserved among other LytTR binding site) indicating that they may play an important role in indirect binding or in allowing for proper po ...
... DNA bases – N201 makes contact via the phosphate backbone. This is interesting because other nucleotides in the binding site are quite important for binding (and conserved among other LytTR binding site) indicating that they may play an important role in indirect binding or in allowing for proper po ...
Pol Is a Candidate for the Mouse Pulmonary
... in adenoma susceptibility between A/J mice and BALB/cJ mice (7). Par2 is mapped to the mouse chromosome 18 and accounts for 60% phenotype variance (7–9). The resistance of BALB/cJ mice appears due to the interaction between the Pas1 QTL and Par2 QTL in the BALB/cJ mouse genome (10). We previously us ...
... in adenoma susceptibility between A/J mice and BALB/cJ mice (7). Par2 is mapped to the mouse chromosome 18 and accounts for 60% phenotype variance (7–9). The resistance of BALB/cJ mice appears due to the interaction between the Pas1 QTL and Par2 QTL in the BALB/cJ mouse genome (10). We previously us ...
PDF - Biotechnology for Biofuels
... largely dependent on characterization of metabolic mechanisms within the cell. The marine diatom Cyclotella cryptica, which was originally identified in the Aquatic Species Program, is a promising strain of microalgae for large-scale production of biofuel and bioproducts, such as omega-3 fatty acids ...
... largely dependent on characterization of metabolic mechanisms within the cell. The marine diatom Cyclotella cryptica, which was originally identified in the Aquatic Species Program, is a promising strain of microalgae for large-scale production of biofuel and bioproducts, such as omega-3 fatty acids ...
Stringent Response in Myxococcus xanthus
... Starve for amino acids Fruiting body formation Starve for amino acids Accumulation of (p)ppGpp Accumulate (p)ppGpp ??Starve for aa Fruiting bodies Is this a causal relationship? Is there a connection between initiation of fruiting body development and (p)ppGpp accumulation? ...
... Starve for amino acids Fruiting body formation Starve for amino acids Accumulation of (p)ppGpp Accumulate (p)ppGpp ??Starve for aa Fruiting bodies Is this a causal relationship? Is there a connection between initiation of fruiting body development and (p)ppGpp accumulation? ...
TRANSPORT PROCESSES
... nascent chains. The ligand for these two lectins, which contains a single glucose residue, is generated by a specific glucosyltransferase in the ER lumen. This enzyme acts only on polypeptide chains that are unfolded or misfolded. Binding of calnexin and calreticulin to unfolded nascent chains preve ...
... nascent chains. The ligand for these two lectins, which contains a single glucose residue, is generated by a specific glucosyltransferase in the ER lumen. This enzyme acts only on polypeptide chains that are unfolded or misfolded. Binding of calnexin and calreticulin to unfolded nascent chains preve ...
Protein - Angelfire
... – Chains fold and twist themselves to form proteins; some of these proteins become enzymes themselves – These enzymes may then be used to break apart or put together other compounds ...
... – Chains fold and twist themselves to form proteins; some of these proteins become enzymes themselves – These enzymes may then be used to break apart or put together other compounds ...
EC->PDB
... have sequence identities >= 30% (but this is not an absolute measure); proteins are placed in superfamilies when, in spite of low sequence identity, their structural and functional characteristics suggest a common evolutionary origin; and proteins are classed as having a common fold if they have the ...
... have sequence identities >= 30% (but this is not an absolute measure); proteins are placed in superfamilies when, in spite of low sequence identity, their structural and functional characteristics suggest a common evolutionary origin; and proteins are classed as having a common fold if they have the ...
The Ethics of Gene Therapy
... defective gene is replaced by a new, functional copy of the gene – Can be accomplished by homologous recombination, although efficiency is low ...
... defective gene is replaced by a new, functional copy of the gene – Can be accomplished by homologous recombination, although efficiency is low ...
Cardiac Energy Dependence on Glucose Increases
... (mTOR), a kinase that drives cardiac growth by promoting protein synthesis and enhancing glucose and lipid metabolism,12 is robustly activated.9 mTORC1 is activated by signals of nutrient availability, including insulin and other growth factors, amino acids, and adequate energy levels (oxygen availa ...
... (mTOR), a kinase that drives cardiac growth by promoting protein synthesis and enhancing glucose and lipid metabolism,12 is robustly activated.9 mTORC1 is activated by signals of nutrient availability, including insulin and other growth factors, amino acids, and adequate energy levels (oxygen availa ...
IOSR Journal of Computer Engineering (IOSR-JCE)
... protein contains a unique amino acid sequence which has a set of generic codes called „codon‟. ‟Codon‟ is a set of three nucleotide as for example AUG is a code that contains adenine-uracil-guanine nucleotides respectively. On the other hand, DNA sequence contains four nucleotides such as adenine (A ...
... protein contains a unique amino acid sequence which has a set of generic codes called „codon‟. ‟Codon‟ is a set of three nucleotide as for example AUG is a code that contains adenine-uracil-guanine nucleotides respectively. On the other hand, DNA sequence contains four nucleotides such as adenine (A ...
Amino Acids - Angelo State University
... • More than 200 peptides have been identified as being essential to the body’s proper functioning. • Vasopressin and oxytocin are nonapeptide hormones secreted by the pituitary gland. Six of the amino acid residues are held in a loop by disulfide bridges formed by the oxidation of two cysteine resid ...
... • More than 200 peptides have been identified as being essential to the body’s proper functioning. • Vasopressin and oxytocin are nonapeptide hormones secreted by the pituitary gland. Six of the amino acid residues are held in a loop by disulfide bridges formed by the oxidation of two cysteine resid ...
Evolution of Cellular Data Processing
... PEP (phosphoenol pyruvate)-powered phosphotransferase systems, abbreviated as PTS. They combine membrane transport with the regulation of gene transcription. Prokaryotes are capable of switching their genes on and off in a specific and signal-controlled mode in order to adapt to the actual condition ...
... PEP (phosphoenol pyruvate)-powered phosphotransferase systems, abbreviated as PTS. They combine membrane transport with the regulation of gene transcription. Prokaryotes are capable of switching their genes on and off in a specific and signal-controlled mode in order to adapt to the actual condition ...
Western Blot - Faperta UGM
... Its cut is predictable and consistent manner at a site within or adjacent to restriction site It require only magnesium ion as cofactor Have both restriction and modification activity Cut at sites closed to recognition site ATP is required ...
... Its cut is predictable and consistent manner at a site within or adjacent to restriction site It require only magnesium ion as cofactor Have both restriction and modification activity Cut at sites closed to recognition site ATP is required ...
A prophage-encoded actin-like protein required for efficient viral
... virion assembly and lysis of the host cell (3,4). Recent reports revealed that viral replication in prokaryotes appears to be organized at specific intracellular locations and this process relies on the action of cytoskeletal proteins (5). Cytoskeletal elements in eu- and prokaryotes are involved in ...
... virion assembly and lysis of the host cell (3,4). Recent reports revealed that viral replication in prokaryotes appears to be organized at specific intracellular locations and this process relies on the action of cytoskeletal proteins (5). Cytoskeletal elements in eu- and prokaryotes are involved in ...
CHAPTER 4 ISOLATION, CHARACTERIZATION AND EXPRESSION OF GA20ox
... 4.1) based on constitutively expressed house-keeping tef Actin and 25S rRNA gene to optimize qRT-PCR measurements. The E. tef 25S rRNA gene was selected and amplification was optimized based on the LightCycler 480 (Roche Diagnostic, UK) result. The crossing point value (CP) was generated representin ...
... 4.1) based on constitutively expressed house-keeping tef Actin and 25S rRNA gene to optimize qRT-PCR measurements. The E. tef 25S rRNA gene was selected and amplification was optimized based on the LightCycler 480 (Roche Diagnostic, UK) result. The crossing point value (CP) was generated representin ...
Osmoadaptative Strategy and Its Molecular
... of halophilic proteins possibly allows them to avoid overly rigid folded conformations. On the other hand, the proteomes of halophiles that use organic solutes as their main osmolytes (salt-out organisms) are not enriched in highly acidic proteins, although they typically produce extracellular prote ...
... of halophilic proteins possibly allows them to avoid overly rigid folded conformations. On the other hand, the proteomes of halophiles that use organic solutes as their main osmolytes (salt-out organisms) are not enriched in highly acidic proteins, although they typically produce extracellular prote ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.