Innovative Purification Protocol for Heparin Binding
... as rHI, under the trade name Humulin [43, 45]. Since then, different biopharmaceuticals products have been developed like blood factors used for treatment of haemophilia, thrombolytic agents used to treat thromboembolism, hematopoietic growth factors used in the treatment of blood related diseases, ...
... as rHI, under the trade name Humulin [43, 45]. Since then, different biopharmaceuticals products have been developed like blood factors used for treatment of haemophilia, thrombolytic agents used to treat thromboembolism, hematopoietic growth factors used in the treatment of blood related diseases, ...
Inositol 1,3,4,5,6-Pentakisphosphate 2-Kinase
... Two parallel phytic acid biosynthetic pathways, one lipid dependent and the other lipid independent, have been proposed for higher plants (Brearley and Hanke, 2000; Stevenson-Paulik et al., 2002; Raboy, 2003). Radioisotope labeling experiments with duckweed (Spirodela polyrhiza), an aquatic plant, s ...
... Two parallel phytic acid biosynthetic pathways, one lipid dependent and the other lipid independent, have been proposed for higher plants (Brearley and Hanke, 2000; Stevenson-Paulik et al., 2002; Raboy, 2003). Radioisotope labeling experiments with duckweed (Spirodela polyrhiza), an aquatic plant, s ...
Autotaxin–Lysophosphatidic Acid Axis Acts Downstream of
... and directional migration by controlling the formation of filopodia/lamellipodia.6 Similarly, carnitine palmitoyltransferase I, a rate-limiting enzyme involved in fatty acid oxidation, was found to regulate EC sprouting by selectively inducing proliferation, ...
... and directional migration by controlling the formation of filopodia/lamellipodia.6 Similarly, carnitine palmitoyltransferase I, a rate-limiting enzyme involved in fatty acid oxidation, was found to regulate EC sprouting by selectively inducing proliferation, ...
Best Plant Protein
... Dietary protein is consumed from myriad food sources, and each contains a unique non-protein content that can have different health related effects aside from their respective contributions on muscle protein synthesis (MPS). 1,2,3 Little argument remains about whole animal, non-milk proteins (so-cal ...
... Dietary protein is consumed from myriad food sources, and each contains a unique non-protein content that can have different health related effects aside from their respective contributions on muscle protein synthesis (MPS). 1,2,3 Little argument remains about whole animal, non-milk proteins (so-cal ...
Nucleic Acids Research
... mitochondria of all eucaryotic cells. It is one of a series of proteins involved in the electron transport chain, and is therefore required for aerobic respiration. Due to structural and functional constraints, the cytochrome c proteins of widely divergent organisms exhibit considerable amino acid h ...
... mitochondria of all eucaryotic cells. It is one of a series of proteins involved in the electron transport chain, and is therefore required for aerobic respiration. Due to structural and functional constraints, the cytochrome c proteins of widely divergent organisms exhibit considerable amino acid h ...
Electrostatic Interactions in Wild
... structure, stability, and dynamics of a folded protein. Despite their obvious importance, the calculation of electrostatic interaction energies in proteins is complicated by the very large number of interacting groups. It is therefore important to make experimental measurements of such interactions ...
... structure, stability, and dynamics of a folded protein. Despite their obvious importance, the calculation of electrostatic interaction energies in proteins is complicated by the very large number of interacting groups. It is therefore important to make experimental measurements of such interactions ...
A Few Good Domains
... The RING finger is a specialized type of Zn finger consisting of 40–60 residues that binds two atoms of zinc, and is involved in mediating protein—protein interactions. The presence of a RING finger domain is a characteristic of RING-class E3 ubiquitin protein ligases capable of transfering ubiquiti ...
... The RING finger is a specialized type of Zn finger consisting of 40–60 residues that binds two atoms of zinc, and is involved in mediating protein—protein interactions. The presence of a RING finger domain is a characteristic of RING-class E3 ubiquitin protein ligases capable of transfering ubiquiti ...
Cloning, Purification, and Partial Characterization of the
... and its integration site [5,6] clearly suggest that it is an intein. The intein is inserted in the middle of the Walker-A motif [7], a highly conserved motif which is found in all members of the AAA+ family of ATPases [8,9]. The sequence of the motif in Halobacterium MCM is GDPGTGKS and the intein i ...
... and its integration site [5,6] clearly suggest that it is an intein. The intein is inserted in the middle of the Walker-A motif [7], a highly conserved motif which is found in all members of the AAA+ family of ATPases [8,9]. The sequence of the motif in Halobacterium MCM is GDPGTGKS and the intein i ...
Proteomic sensitivity to dietary manipulations in rainbow trout
... fish. A proteomics approach was used to study the protein profiles of livers of rainbow trout that have been fed two diets containing different proportions of plant ingredients. Both diets control (C) and soy (S) contained fish meal and plant ingredients and synthetic amino acids, but diet S had a g ...
... fish. A proteomics approach was used to study the protein profiles of livers of rainbow trout that have been fed two diets containing different proportions of plant ingredients. Both diets control (C) and soy (S) contained fish meal and plant ingredients and synthetic amino acids, but diet S had a g ...
Pupko_pairwise
... Query sequence: DNA or protein? • For coding sequences, we can use the DNA sequence or the protein sequence to search for similar sequences. • Which is preferable? ...
... Query sequence: DNA or protein? • For coding sequences, we can use the DNA sequence or the protein sequence to search for similar sequences. • Which is preferable? ...
Gel electrophoresis - Caltech Particle Theory
... You have to remember that when we work with proteins, we work with many copies of each kind of protein. As a result, the collection of proteins of any given size tend to move through the gel at the same rate, even if they do not take exactly the same tunnels to get through. Back to our analogy of th ...
... You have to remember that when we work with proteins, we work with many copies of each kind of protein. As a result, the collection of proteins of any given size tend to move through the gel at the same rate, even if they do not take exactly the same tunnels to get through. Back to our analogy of th ...
Host Factors in the Replication of Positive
... Following translation and processing of viral proteins, (+)RNA viruses adopt a relatively conserved process for completing the RNA replication cycle in host cells. Studies of the mechanisms of (+)RNA virus replication have suggested that this process usually involves the following steps: 1) selectin ...
... Following translation and processing of viral proteins, (+)RNA viruses adopt a relatively conserved process for completing the RNA replication cycle in host cells. Studies of the mechanisms of (+)RNA virus replication have suggested that this process usually involves the following steps: 1) selectin ...
Gene–Nutrition Interaction in Human Performance and Exercise
... remained highly concordant throughout a lifetime, physical activity level did not, suggesting that environmental factors rather than genetic factors are more important in determining an individual’s propensity to be active in later life. Recently, Maia et al.4 studied 411 Portuguese twins of differe ...
... remained highly concordant throughout a lifetime, physical activity level did not, suggesting that environmental factors rather than genetic factors are more important in determining an individual’s propensity to be active in later life. Recently, Maia et al.4 studied 411 Portuguese twins of differe ...
Identification of Plant Virus Movement-Host Protein
... (G em iniviridae), reverse transcriptase ( Caulimoviridae) or RNA-dependent R N A polymerase (for multiplication and transcription of RNA virus ge nom es), and iii.) a movement protein (M P), essen tial for intra- and intercellular motion of the virus to enable systemic infection of the whole plan ...
... (G em iniviridae), reverse transcriptase ( Caulimoviridae) or RNA-dependent R N A polymerase (for multiplication and transcription of RNA virus ge nom es), and iii.) a movement protein (M P), essen tial for intra- and intercellular motion of the virus to enable systemic infection of the whole plan ...
One amino acid makes the difference: the formation of ent
... wide array of important functions. With over 60,000 different structures described at present, terpenoids represent the largest and structurally most diverse group of natural products [1]. This biodiversity arises from only a few prenyl diphosphate precursors. Terpene synthases (TPSs), the key enzym ...
... wide array of important functions. With over 60,000 different structures described at present, terpenoids represent the largest and structurally most diverse group of natural products [1]. This biodiversity arises from only a few prenyl diphosphate precursors. Terpene synthases (TPSs), the key enzym ...
The diagram below shows the process of translation. PROCTOR
... (C) All of the amino acids after the first will be changed in the polypeptide, because the deletion will create a shift beginning with the second, and this will change all codons that follow. Distractor Rationale: This answer suggests the student may understand that most deletions result in a frames ...
... (C) All of the amino acids after the first will be changed in the polypeptide, because the deletion will create a shift beginning with the second, and this will change all codons that follow. Distractor Rationale: This answer suggests the student may understand that most deletions result in a frames ...
Identification of Plant Virus Movement
... by it. Subcellular fractionation studies suggested that the tubules were derived from the endoplas mic reticulum (E R ), implying that SqLCV recruits the E R as a conduit for cell-to-cell movement in a developmental manner. Several features of the BL1 induced tubules appear to distinguish them from ...
... by it. Subcellular fractionation studies suggested that the tubules were derived from the endoplas mic reticulum (E R ), implying that SqLCV recruits the E R as a conduit for cell-to-cell movement in a developmental manner. Several features of the BL1 induced tubules appear to distinguish them from ...
Conserved Key Amino Acid Positions (CKAAPs) Derived From the
... homologues in SWALL, a nonredundant protein sequence database. The most similar sequences are purged into a common frequency matrix, and weighted homologues of each one of the subsequences are used in scoring for conserved key amino acid positions (CKAAPs). We have set the top 20% of the high-scorin ...
... homologues in SWALL, a nonredundant protein sequence database. The most similar sequences are purged into a common frequency matrix, and weighted homologues of each one of the subsequences are used in scoring for conserved key amino acid positions (CKAAPs). We have set the top 20% of the high-scorin ...
p53
... - T antigen oncogenic activity was carried out by sequestering p53 when the latter was supposed to inhibit tumor progression - T antigen binds also to pRb, sequestering it from negatively regulating cell cycle progression ...
... - T antigen oncogenic activity was carried out by sequestering p53 when the latter was supposed to inhibit tumor progression - T antigen binds also to pRb, sequestering it from negatively regulating cell cycle progression ...
Molecular Characterization of a Hamster Oviduct
... BabOGP cDNA probe [20]. The BabOGP cDNA was partially amplified by the polymerase chain reaction (PCR) in the presence of DIG-11-dUTP. Based on the DNA sequence data described by Donnelly et al. [20], two oligonucleotides (a part of the sense or anti-sense sequence of a baboon oviduct-specific glyco ...
... BabOGP cDNA probe [20]. The BabOGP cDNA was partially amplified by the polymerase chain reaction (PCR) in the presence of DIG-11-dUTP. Based on the DNA sequence data described by Donnelly et al. [20], two oligonucleotides (a part of the sense or anti-sense sequence of a baboon oviduct-specific glyco ...
The Basics of RT-PCR
... tubes in the same run. This type of contamination is thus insidious, because the negative control tubes are often “clean;” therefore, unexpected positive results can be caused simply by contamination. If a contamination problem is suspected, it is sometimes helpful to perform multiple negative-contr ...
... tubes in the same run. This type of contamination is thus insidious, because the negative control tubes are often “clean;” therefore, unexpected positive results can be caused simply by contamination. If a contamination problem is suspected, it is sometimes helpful to perform multiple negative-contr ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)
... twenty amino acids. The main chains of all these amino acids are identical. The difference in the side chain in these 20 amino acids incorporates diversity in structure-function relationship in proteins adapted in different environments. Substitution, deletion and insertion are the available mechani ...
... twenty amino acids. The main chains of all these amino acids are identical. The difference in the side chain in these 20 amino acids incorporates diversity in structure-function relationship in proteins adapted in different environments. Substitution, deletion and insertion are the available mechani ...
Module 1 : Introduction to the study of man
... 5.1.1 Draw and label the molecular components of the cell membrane. 5.1.2 Explain how the alteration of the fatty acid composition and cholesterol content may alter the structure and hence the functions of the membrane. 5.1.3 Describe the different location of proteins on the cell membrane and their ...
... 5.1.1 Draw and label the molecular components of the cell membrane. 5.1.2 Explain how the alteration of the fatty acid composition and cholesterol content may alter the structure and hence the functions of the membrane. 5.1.3 Describe the different location of proteins on the cell membrane and their ...
Identification of four small molecular mass proteins in the silk of
... sequencing of the two clones identified the complete 5′ and 3′ ends. The clones were identical save position 329 (3′ UTR) where T was identified in one and G in the other clone. The BmSPI 2 cDNA is 411 nt long and includes 14 nt 5′ UTR, 198 nt ORF, and 199 nt 3′ UTR with three overlapping polyadenyl ...
... sequencing of the two clones identified the complete 5′ and 3′ ends. The clones were identical save position 329 (3′ UTR) where T was identified in one and G in the other clone. The BmSPI 2 cDNA is 411 nt long and includes 14 nt 5′ UTR, 198 nt ORF, and 199 nt 3′ UTR with three overlapping polyadenyl ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.