Releasable conjugation of polymers to proteins
... mPEG was added drop-wise to papain during the conjugation step, the recovered product did not contain any detectable conjugate. The authors rationalize this to dimerization of papain caused by nucleophilic attack of the thiol on papain on the papain–mPEG bio-conjugate. This observation supports evid ...
... mPEG was added drop-wise to papain during the conjugation step, the recovered product did not contain any detectable conjugate. The authors rationalize this to dimerization of papain caused by nucleophilic attack of the thiol on papain on the papain–mPEG bio-conjugate. This observation supports evid ...
Unit 04 Lecture Notes - Roderick Anatomy and Physiology
... • I can explain the process of Translation. (Where, what molecules are involved and why it’s important) ...
... • I can explain the process of Translation. (Where, what molecules are involved and why it’s important) ...
7.06 Cell Biology EXAM #3
... And be sure to put your NAME ON EACH PAGE in case they become separated! There are SEVEN pages including this cover sheet. ...
... And be sure to put your NAME ON EACH PAGE in case they become separated! There are SEVEN pages including this cover sheet. ...
Aspartic acid or Glutamic Acid Histidine
... shown on the right: Choice B: Explain the difference in entropy (∆So) between the wild-type protein and the Phe57→Val mutant, i.e. why is the overall entropy change larger for the mutant protein. The overall entropy change is higher because the non-polar surface area of Val is smaller than Phe (3 pt ...
... shown on the right: Choice B: Explain the difference in entropy (∆So) between the wild-type protein and the Phe57→Val mutant, i.e. why is the overall entropy change larger for the mutant protein. The overall entropy change is higher because the non-polar surface area of Val is smaller than Phe (3 pt ...
Cytochrome P450 - Spektrum der Wissenschaft
... physically interact in vivo. We adapted this system to our purposes so that it can be used as a screening-system for adrenodoxin mutants and cytochrome P450 mutants to detect proteins with increased interaction to their respective partners. Different adrenodoxin mutants were generated by site-direct ...
... physically interact in vivo. We adapted this system to our purposes so that it can be used as a screening-system for adrenodoxin mutants and cytochrome P450 mutants to detect proteins with increased interaction to their respective partners. Different adrenodoxin mutants were generated by site-direct ...
Classification and substrate head-group specificity of membrane
... been postulated that the Δ9 desaturases are the most ancient desaturases among the three groups because of their universal distribution in organisms [24]. The FDs utilize acyl-CoA as substrates except for the FDs of plant plastids [8]. Third, the methyl-end (ME) desaturases are responsible for intro ...
... been postulated that the Δ9 desaturases are the most ancient desaturases among the three groups because of their universal distribution in organisms [24]. The FDs utilize acyl-CoA as substrates except for the FDs of plant plastids [8]. Third, the methyl-end (ME) desaturases are responsible for intro ...
Resistance to the Pseudomonas syringae Effector
... 2004) for mutants susceptible to DC3000(hopA1) (see “Materials and Methods”). We screened approximately 25,000 M2 plants representing 1,100 M1 plants from 67 different pools by dip inoculation with DC3000(hopA1). Of 138 M2 individuals that were scored as susceptible after the first screen, we identi ...
... 2004) for mutants susceptible to DC3000(hopA1) (see “Materials and Methods”). We screened approximately 25,000 M2 plants representing 1,100 M1 plants from 67 different pools by dip inoculation with DC3000(hopA1). Of 138 M2 individuals that were scored as susceptible after the first screen, we identi ...
Integrated search and alignment of protein structures
... constructs a BLOSUM-like substitution matrix for this backbone angle alphabet, so that BLAST algorithm can be used without any modifications. Currently available protein structure search methods provide database filtering, but defer a detailed structural alignment to further analysis by external ali ...
... constructs a BLOSUM-like substitution matrix for this backbone angle alphabet, so that BLAST algorithm can be used without any modifications. Currently available protein structure search methods provide database filtering, but defer a detailed structural alignment to further analysis by external ali ...
Protein thermal stability: insights from atomic displacement
... smearing of atomic electron densities around their equilibrium positions due to thermal motion and positional disorder. Analysis of B values, therefore, is likely to provide newer insights into protein dynamics, flexibility of amino acids and protein stability. Molecular dynamics studies have sugges ...
... smearing of atomic electron densities around their equilibrium positions due to thermal motion and positional disorder. Analysis of B values, therefore, is likely to provide newer insights into protein dynamics, flexibility of amino acids and protein stability. Molecular dynamics studies have sugges ...
Identification of a putative flexible loop in Arabidopsis glutathione
... cysteinyl carboxyl group. Attack of this acylphosphate by the αamino of glycine results in a tetrahedral intermediate, followed by the release of phosphate and the formation of a peptide bond [7]. Other enzymes with similar reaction mechanisms include γglutamylcysteine synthetase and -alanine : -a ...
... cysteinyl carboxyl group. Attack of this acylphosphate by the αamino of glycine results in a tetrahedral intermediate, followed by the release of phosphate and the formation of a peptide bond [7]. Other enzymes with similar reaction mechanisms include γglutamylcysteine synthetase and -alanine : -a ...
Development of the Musculoskeletal System
... • induced by expression of BMP4 and FGFs from lateral plate • MYF5 expression Æ epimere Æ extensor muscles of axial skeleton • BMP4 expression by ectoderm induces dorsal neural tube expression of WNT, which induces MYF5 expression • MYO-D and MYF5 induce expression of myogenin and MRF5 involved in f ...
... • induced by expression of BMP4 and FGFs from lateral plate • MYF5 expression Æ epimere Æ extensor muscles of axial skeleton • BMP4 expression by ectoderm induces dorsal neural tube expression of WNT, which induces MYF5 expression • MYO-D and MYF5 induce expression of myogenin and MRF5 involved in f ...
$doc.title
... known to accumulate in the vacuoles of petal epidermal cells. Conditions inside the vacuole are therefore of utmost importance in determining the exact structure and colour of anthocyan ins. Recent evidence suggests that anthocyanins are tagged for transport into the vacuole by glutathione S-transfe ...
... known to accumulate in the vacuoles of petal epidermal cells. Conditions inside the vacuole are therefore of utmost importance in determining the exact structure and colour of anthocyan ins. Recent evidence suggests that anthocyanins are tagged for transport into the vacuole by glutathione S-transfe ...
Cloning and Expression of Bovine Sodium/Glucose Cotransporters* J. Dairy Sci. 88:182–194
... of the SGLT family (Mackenzie et al., 1994). However, its function may need to be reevaluated because human SGLT3 apparently does not transport glucose (Wright and Turk, 2004). Little is known about the isoforms SGLT4 to 6, except that SGLT4 and 6 are expressed in a wide variety of tissues, whereas ...
... of the SGLT family (Mackenzie et al., 1994). However, its function may need to be reevaluated because human SGLT3 apparently does not transport glucose (Wright and Turk, 2004). Little is known about the isoforms SGLT4 to 6, except that SGLT4 and 6 are expressed in a wide variety of tissues, whereas ...
Projection Structure of a Plant Vacuole Membrane Aquaporin by
... hydrophobic transmembrane domains and an AsnPro-Ala consensus sequence (often termed the ``NPA box''), which is duplicated between the two halves of the protein. Many proteins of the MIP family form channels for small, generally non-ionic solutes. Aquaporins (AQPs) are a subset of these, the term be ...
... hydrophobic transmembrane domains and an AsnPro-Ala consensus sequence (often termed the ``NPA box''), which is duplicated between the two halves of the protein. Many proteins of the MIP family form channels for small, generally non-ionic solutes. Aquaporins (AQPs) are a subset of these, the term be ...
Use of the non-radioactive SUnSET method to detect decreased
... provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/ publicdomain/zero/1.0/) applies to the data made availab ...
... provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/ publicdomain/zero/1.0/) applies to the data made availab ...
Ontogenomic study of the relationship between number of gene
... Motivation: Splice variation plays important roles in evolution and cancer. Different splice variants of a gene may be characteristic of particular cellular processes, subcellular locations or organs. Although several genomic projects have identified splice variants, there have been no large-scale co ...
... Motivation: Splice variation plays important roles in evolution and cancer. Different splice variants of a gene may be characteristic of particular cellular processes, subcellular locations or organs. Although several genomic projects have identified splice variants, there have been no large-scale co ...
what we`re reading
... eight Gainer – Looking to bulk up? Weight gainer combines protein, often whey, with a mix of high-carbohydrate ingredients that makes it much more calorie-dense than typical protein powders. It is often used by bodybuilders who are looking to pack on the pounds, or by serious athletes who have dif ...
... eight Gainer – Looking to bulk up? Weight gainer combines protein, often whey, with a mix of high-carbohydrate ingredients that makes it much more calorie-dense than typical protein powders. It is often used by bodybuilders who are looking to pack on the pounds, or by serious athletes who have dif ...
Ten remarks on peptide bond formation on the ribosome
... with a direct role of A2451 in catalysis. However, mutation of G2447 had no observable effects on cell growth and was non-essential for peptide bond formation [31,32,34]. This result does not support the proposed charge relay mechanism involving G2447 and the role of A2451 as a general base in the c ...
... with a direct role of A2451 in catalysis. However, mutation of G2447 had no observable effects on cell growth and was non-essential for peptide bond formation [31,32,34]. This result does not support the proposed charge relay mechanism involving G2447 and the role of A2451 as a general base in the c ...
2006 Program
... “Gelatinase contributes to the release of extracellular DNA in the development of Enterococcus faecalis biofilms” Lance R. Thurlow and Lynn E. Hancock “The genetic basis for capsular polysaccharide production in Enterococcus faecalis serogroups C and D” ...
... “Gelatinase contributes to the release of extracellular DNA in the development of Enterococcus faecalis biofilms” Lance R. Thurlow and Lynn E. Hancock “The genetic basis for capsular polysaccharide production in Enterococcus faecalis serogroups C and D” ...
mbe.oxfordjournals.org - Oxford Academic
... et al. 2005). Analogous to all other nuclear proteins, eukaryotic ribosomal proteins are synthesized in the cytoplasm, subsequently imported through the nuclear pore complex into the nucleoplasm, and ultimately accumulate in the nucleolus (Fromont-Racine et al. 2003). Their association with the newl ...
... et al. 2005). Analogous to all other nuclear proteins, eukaryotic ribosomal proteins are synthesized in the cytoplasm, subsequently imported through the nuclear pore complex into the nucleoplasm, and ultimately accumulate in the nucleolus (Fromont-Racine et al. 2003). Their association with the newl ...
A unique amino acid substitution, T126I, in human
... with an increased risk of developing hepatocellular carcinoma (HCC), which is one of the major causes of human death. HBV is a double-stranded DNA virus with a very compact genome of only about 3200 bp. It encodes four proteins: S, P, C and X. Some regions of the genome encode two proteins using dif ...
... with an increased risk of developing hepatocellular carcinoma (HCC), which is one of the major causes of human death. HBV is a double-stranded DNA virus with a very compact genome of only about 3200 bp. It encodes four proteins: S, P, C and X. Some regions of the genome encode two proteins using dif ...
Differential Accumulation Pattern of Met-rich beta
... callus after 6-weeks and they are regenerated into plantlets ...
... callus after 6-weeks and they are regenerated into plantlets ...
Specific Growth Inhibition by Acetate of an Escherichia coli Strain
... (Chen et al., 1999). It consists of two domains: a GTPbinding N-terminal domain whose three dimensional structure is similar to those of the GTPase domains of other G-proteins (Bourne et al., 1991) and a C-terminal domain which is conserved within the Era subfamily, containing a putative KH-like RNA ...
... (Chen et al., 1999). It consists of two domains: a GTPbinding N-terminal domain whose three dimensional structure is similar to those of the GTPase domains of other G-proteins (Bourne et al., 1991) and a C-terminal domain which is conserved within the Era subfamily, containing a putative KH-like RNA ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.