Complementary DNA Sequence of a Human Cytoplasmic Actin
... actin cDNA clone, large-scale plasmid preparations and DNA fragment isolations were carried out as described (Hanukoglu & Fuchs, 1982). The DNA sequencing strategy used for this cDNA is shown in Figure 1. The DNA sequence and predicted amino acid sequence of the actin cDNA insert are shown in Figure ...
... actin cDNA clone, large-scale plasmid preparations and DNA fragment isolations were carried out as described (Hanukoglu & Fuchs, 1982). The DNA sequencing strategy used for this cDNA is shown in Figure 1. The DNA sequence and predicted amino acid sequence of the actin cDNA insert are shown in Figure ...
Protein
... order specified by the genetic code responsible for synthesizing the protein If an (essential) amino acid is unavailable, protein synthesis is ...
... order specified by the genetic code responsible for synthesizing the protein If an (essential) amino acid is unavailable, protein synthesis is ...
Formate Dehydrogenase, an Enzyme of Anaerobic Metabolism, Is
... The induction of Fdh expression required 1 d of Fe deficiency, with the amount of Fdh mRNA increasing gradually day by day (Fig. 7). After 14 d, Fdh expression reached a maximum and remained constant for 28 d. When Fe was resupplied in the form of Fe-EDTA to the culture solution of Fe-deficient plan ...
... The induction of Fdh expression required 1 d of Fe deficiency, with the amount of Fdh mRNA increasing gradually day by day (Fig. 7). After 14 d, Fdh expression reached a maximum and remained constant for 28 d. When Fe was resupplied in the form of Fe-EDTA to the culture solution of Fe-deficient plan ...
CS273_SequenceSimilarity1
... • Nucleic acids and proteins are related by molecular evolution Orthologs: two proteins in animals X and Y that evolved from one protein in immediate ancestor animal Z Paralogs: two proteins that evolved from one protein through duplication in some ancestor Homologs: orthologs or paralogs that ...
... • Nucleic acids and proteins are related by molecular evolution Orthologs: two proteins in animals X and Y that evolved from one protein in immediate ancestor animal Z Paralogs: two proteins that evolved from one protein through duplication in some ancestor Homologs: orthologs or paralogs that ...
TRIzol Reagent
... (back to Table of Contents) (back to Protocol and Application Notes) (back to RNA Isolation) Incubate sample for 5 minutes in TRIzol at room temperature. Add 0.2 ml of chloroform for every 1ml of TRIzol used. Shake vigorously for 15 seconds and incubate at room temperature for 2-3 min. Centrifuge sa ...
... (back to Table of Contents) (back to Protocol and Application Notes) (back to RNA Isolation) Incubate sample for 5 minutes in TRIzol at room temperature. Add 0.2 ml of chloroform for every 1ml of TRIzol used. Shake vigorously for 15 seconds and incubate at room temperature for 2-3 min. Centrifuge sa ...
Topology and Phosphorylation of Soybean Nodulin
... acids are the primary carbon source supplied to the bacteroids by the plant (see Dilworth and Glenn, 1984). The flow of these compounds is controlled by the PBM. Several carbon and amino acid transport systems have been identiffed in the PBM using isolated peribacteroid units (Day et al., 1990). Non ...
... acids are the primary carbon source supplied to the bacteroids by the plant (see Dilworth and Glenn, 1984). The flow of these compounds is controlled by the PBM. Several carbon and amino acid transport systems have been identiffed in the PBM using isolated peribacteroid units (Day et al., 1990). Non ...
Protein-protein interaction networks (I): data preprocessing
... (such as MIPS). Each point in the graph represents an entire interaction data set and its position specifies coverage and accuracy (on a log-log scale). Notably the comparison is incomplete since the reference set is still incomplete, and may suffer from biases. However, we can see that there are la ...
... (such as MIPS). Each point in the graph represents an entire interaction data set and its position specifies coverage and accuracy (on a log-log scale). Notably the comparison is incomplete since the reference set is still incomplete, and may suffer from biases. However, we can see that there are la ...
Nucleotide Sequence of the DNA Complementary to Avian (Chicken
... It was not surprising to find that the greatest conservation in sequence homology was in the amino terminal 1-34 portion of the hormones, since this region has been shown to be responsible for the biological activity of PTH (2, 3). Within this region, the amino-terminal Ser-Val sequence is identical ...
... It was not surprising to find that the greatest conservation in sequence homology was in the amino terminal 1-34 portion of the hormones, since this region has been shown to be responsible for the biological activity of PTH (2, 3). Within this region, the amino-terminal Ser-Val sequence is identical ...
VegT initiates endoderm formation
... 1999b). The region constituting the organizer overlaps a portion of anterior endomesoderm and is where organizer genes such as Xlim1, a gene encoding a LIM class homeodomain protein, are expressed (Taira et al., 1992). The gene encoding the transcription factor Xlhbox8 (Wright et al., 1988) shows re ...
... 1999b). The region constituting the organizer overlaps a portion of anterior endomesoderm and is where organizer genes such as Xlim1, a gene encoding a LIM class homeodomain protein, are expressed (Taira et al., 1992). The gene encoding the transcription factor Xlhbox8 (Wright et al., 1988) shows re ...
Patterns of prokaryotic lateral gene transfers affecting
... ’TMDs ≥ 4’ or ‘TMDs 1-3’ refers to the number of TMDs predicted on protein sequences. Transporters typically have at least four TMDs (TMDs ≥ 4). Proteins with one to three TMDs represent putative membrane proteins. d EC numbers were annotated for each entry based on a significant sequence similarity ...
... ’TMDs ≥ 4’ or ‘TMDs 1-3’ refers to the number of TMDs predicted on protein sequences. Transporters typically have at least four TMDs (TMDs ≥ 4). Proteins with one to three TMDs represent putative membrane proteins. d EC numbers were annotated for each entry based on a significant sequence similarity ...
Acetyl-coenzyme A carboxylase – an attractive enzyme for biotechnolo
... the impact of its product on cellular processes in this group of organisms. Escherichia coli, the model bacteria of well-studied biochemistry and molecular biology as well as a “working horse” of biotechnology, provides some introductory information on ACCase relations with other biochemical pathway ...
... the impact of its product on cellular processes in this group of organisms. Escherichia coli, the model bacteria of well-studied biochemistry and molecular biology as well as a “working horse” of biotechnology, provides some introductory information on ACCase relations with other biochemical pathway ...
Infrared spectroscopy: a tool for protein characterization Chenge Li
... SERCA1a is composed of a single polypeptide chain that contains 994 amino acids and has a molecular mass of 110 kDa30,31. The first three-dimensional (3D) structure of SERCA1a was solved by Toyoshima et al. in 200031. The 3D structure shows a 10 helix (M1-M10) transmembrane domain and 3 globular dom ...
... SERCA1a is composed of a single polypeptide chain that contains 994 amino acids and has a molecular mass of 110 kDa30,31. The first three-dimensional (3D) structure of SERCA1a was solved by Toyoshima et al. in 200031. The 3D structure shows a 10 helix (M1-M10) transmembrane domain and 3 globular dom ...
... The tertiary structure of caffeine 1N-demethylase was predicted using fold recognition server and the template used was 1ndo (pdbid). Figure 5 represents the predicted structure of caffeine demethylase. Based on the predicted structure, the protein is organized into a domain containing a six membere ...
Recombinant DNA Technology
... compatible host cells. In order to be propagated, the recombinant DNA molecule (insert DNA joined to vector DNA) must be introduced into a compatible host cell where it can replicate. The direct uptake of foreign DNA by a host cell is called genetic transformation (or transformation). Recombinant DN ...
... compatible host cells. In order to be propagated, the recombinant DNA molecule (insert DNA joined to vector DNA) must be introduced into a compatible host cell where it can replicate. The direct uptake of foreign DNA by a host cell is called genetic transformation (or transformation). Recombinant DN ...
PDF - World Wide Journals
... sodium sulfide (0.5 M) solution. This concluded that 0.5 M sodium sulfide could has reducing capability of chicken feather. The presence of protein is confirmed by biuret test. The solution turned purple after reagent is added and this is only possible, if peptides are present in it. The technique w ...
... sodium sulfide (0.5 M) solution. This concluded that 0.5 M sodium sulfide could has reducing capability of chicken feather. The presence of protein is confirmed by biuret test. The solution turned purple after reagent is added and this is only possible, if peptides are present in it. The technique w ...
Acetyl-coenzyme A carboxylase – an attractive enzyme for biotechnolo
... the impact of its product on cellular processes in this group of organisms. Escherichia coli, the model bacteria of well-studied biochemistry and molecular biology as well as a “working horse” of biotechnology, provides some introductory information on ACCase relations with other biochemical pathway ...
... the impact of its product on cellular processes in this group of organisms. Escherichia coli, the model bacteria of well-studied biochemistry and molecular biology as well as a “working horse” of biotechnology, provides some introductory information on ACCase relations with other biochemical pathway ...
BIOINFORMATICS ORIGINAL PAPER Going from where to why—interpretable prediction of protein subcellular localization
... the predicted class, compared to the class where this feature value is least likely. In contrast, the opposition score is always negative. Given a very low opposition score, it is more likely to observe this feature in a class that was not predicted. Hence, a prediction based on the feature alone wo ...
... the predicted class, compared to the class where this feature value is least likely. In contrast, the opposition score is always negative. Given a very low opposition score, it is more likely to observe this feature in a class that was not predicted. Hence, a prediction based on the feature alone wo ...
Structure and Physiological significance of lipid
... In a selection, cells are grown under conditions in which only transformed cells can survive; all the other cells die. In contrast, in a screen, transformed cells have to be individually tested for the presence of the desired recombinant DNA. Normally, a number of colonies of cells are first sel ...
... In a selection, cells are grown under conditions in which only transformed cells can survive; all the other cells die. In contrast, in a screen, transformed cells have to be individually tested for the presence of the desired recombinant DNA. Normally, a number of colonies of cells are first sel ...
DNA and RNA Extraction Controls Performance Summary
... developed a DNA Extraction Control (DEC) and RNA Extraction Control (REC), which more closely mimic the test sample, as compared to spike controls. Genetic material from the test sample and the DEC or REC is simultaneously extracted by common extraction methods, with the extraction control being as ...
... developed a DNA Extraction Control (DEC) and RNA Extraction Control (REC), which more closely mimic the test sample, as compared to spike controls. Genetic material from the test sample and the DEC or REC is simultaneously extracted by common extraction methods, with the extraction control being as ...
Molecular Cloning and Expression Analysis of IgD in Nile
... comprise the major lymphoid organs of fish, yet bone marrow and lymph nodes constitute the immune organs of mammals. The fish immune system is far simpler than that of primates and rodents [8]. It is necessary to analyze IgD in teleosts, as it will provide a reference for further research in this fi ...
... comprise the major lymphoid organs of fish, yet bone marrow and lymph nodes constitute the immune organs of mammals. The fish immune system is far simpler than that of primates and rodents [8]. It is necessary to analyze IgD in teleosts, as it will provide a reference for further research in this fi ...
nutrient composition of dandelions and its potential as human food
... Two thirds of the world’s populations are suffering from protein malnutrition and about 36 million people die every year due to hunger. Expansion of present agriculture practices into marginal land is not expected to solve the problem of increasing the food supply. New methods of feeding the ever in ...
... Two thirds of the world’s populations are suffering from protein malnutrition and about 36 million people die every year due to hunger. Expansion of present agriculture practices into marginal land is not expected to solve the problem of increasing the food supply. New methods of feeding the ever in ...
Transcriptomic analysis reveals metabolic switches and
... organization and transcription. The genome of trypanosomatids is organized in clusters of protein-coding genes located on the same DNA strand, separated by relatively short intergenic regions (Daniels, Gull & Wickstead, 2010). With a few exceptions, genes do not contain introns, and the clusters are ...
... organization and transcription. The genome of trypanosomatids is organized in clusters of protein-coding genes located on the same DNA strand, separated by relatively short intergenic regions (Daniels, Gull & Wickstead, 2010). With a few exceptions, genes do not contain introns, and the clusters are ...
Histidine Biosynthetic Pathway and Genes: Structure
... that one of the two substrates of the transferase, PRPP, brings about a dissociation of hexamers and higher aggregates, with a resulting increase in the concentration of dimers. On the other hand, ATP counteracts PRPP in this respect. This finding is in apparent conflict with data indicating that th ...
... that one of the two substrates of the transferase, PRPP, brings about a dissociation of hexamers and higher aggregates, with a resulting increase in the concentration of dimers. On the other hand, ATP counteracts PRPP in this respect. This finding is in apparent conflict with data indicating that th ...
The Structure and Topology of Protein Serine/Threonine
... EF hand motifs, whereas in KAPP-1, a kinase interaction domain associated with a phosphorylated receptor precedes the phosphatase domain. Other less closely related examples include the Ca2 stimulated mitochondrial pyruvate dehydrogenase phosphatase, which contains a catalytic subunit sharing 22 pe ...
... EF hand motifs, whereas in KAPP-1, a kinase interaction domain associated with a phosphorylated receptor precedes the phosphatase domain. Other less closely related examples include the Ca2 stimulated mitochondrial pyruvate dehydrogenase phosphatase, which contains a catalytic subunit sharing 22 pe ...
Preparation of cell lysates For immunoblotting, 5 x 105
... used rather than the commercially available anti-FKBP12 antibody C-19 because SA-169 appeared to be more sensitive to rabbit antigen. Although this antibody does not discriminate between FKBP12 and FKBP12.6 isoforms, it is still possible to distinguish the two FKBPs on the basis of their electrophor ...
... used rather than the commercially available anti-FKBP12 antibody C-19 because SA-169 appeared to be more sensitive to rabbit antigen. Although this antibody does not discriminate between FKBP12 and FKBP12.6 isoforms, it is still possible to distinguish the two FKBPs on the basis of their electrophor ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.