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2016 department of medicine research day
2016 department of medicine research day

... formation, gene expression of intestinal fatty acid transporters and tight junction (TJ) proteins and hepatic gene expression of key enzymes in the energy metabolism and fat oxidation to elucidate how decaffeinated polyphenol (P) extracts from GT and BT induce weight loss. Design: 48 C57BL/6 male mi ...
Amino Acids
Amino Acids

... Seager SL, Slabaugh MR, Chemistry for Today: General, Organic and Biochemistry, 7 th Edition, 2011; Berg JM, Tymoczko JL, Stryer L, Biochemistry, 5th Edition, 2002 ...
Chapter 2
Chapter 2

... -pyrimidine –single ring structure Nucleotides are covalently bonded between phosphate groups and either ribose or deoxyribose (depending on which nucleic acid) by phosphodiester bond 2 Types of Nucleic Acids I. RNA (ribonucleic acid) -functions in protein synthesis -usually single stranded -complem ...
Introduction 1
Introduction 1

... Lk = linking number: Number of times one edge of ribbon linked around other – topological property  cannot change w/o cutting. (calculate by Lk = Tw+Wr) Twist = winding of Watson around Crick – integrated angle of twist/2p along length, not an integer, necessarily (calculate by Tw = (number of base ...
Assessment Statement
Assessment Statement

... IB says: Originally, it was assumed that one gene would invariably code for one polypeptide, but many exceptions have been discovered. ...
manual HiScribe T7 In Vitro Transcription Kit E2030
manual HiScribe T7 In Vitro Transcription Kit E2030

... ~1.5 mg per ml of transcription reaction should be easily obtainable using the HiScribe T7 In Vitro Transcription Kit. A 40 µl pilot reaction should be carried out initially to test the quality of DNA template and transcription reagents. Reactions can then be scaled up accordingly as required by the ...
Comparative Proteomics Kit I: Protein Profiler Module
Comparative Proteomics Kit I: Protein Profiler Module

... – Novel traits – Positive, negative, or no effects • Genetic diversity provides pool for natural selection = evolution ...
Reorientation (AMA-1)
Reorientation (AMA-1)

... erythrocytes. Knobs mediate cytoadherence to endothelial cells. ...
Ch 5 Activity List File
Ch 5 Activity List File

... 9. Identify an ester linkage and describe how it is formed. 10. Distinguish between saturated and unsaturated fats. 11. Name the principal energy storage molecules of plants and animals. 12. Distinguish between a protein and a polypeptide. 13. Explain how a peptide bond forms between two amino acids ...
Export To Word
Export To Word

... This lesson will clear misconceptions and probe student thinking by utilizing differentiated instruction and implementing meaningful learning. The educator will also be able to provide students with real world examples. Students are given multiple opportunities to excel and demonstrate their content ...
Biology DNA MCAS questions
Biology DNA MCAS questions

... In phenylketonuria (PKU), an enzyme that converts one amino acid into another does not work properly. Which of the following is the most likely cause of this genetic condition? A. an error in the transcription of the gene for the enzyme B. a mutation in the DNA sequence that codes for the enzyme C. ...
F212 2.1.1 Biological Molecules Proteins
F212 2.1.1 Biological Molecules Proteins

... • Biuret is made up of sodium hydroxide and copper sulphate, when these are added to protein they will turn lilac in colour. • Which solution contains proteins? ...
Plant Transposable Elements
Plant Transposable Elements

...  They are found in all plant species o Greater than 50% of the DNA content of some genomes o Larger genomes have more TEs from: Tenaillon et al (2010) Trends in Plant Science 15:471 ...
Master Dissertation Project
Master Dissertation Project

... immunosupressed population. Alteration in host cellular processes due to infection leaves unique chemical fingerprints in the form of proteins, small molecules and metabolites. Although some potential TB biomarker have been suggested, an extensive differential proteomic characterization should be pe ...
Biochem Review
Biochem Review

... 8. They are ___________ - they can be used over, and over again because they are not destroyed and their shape does not change 9. They are ____________ - SHAPE MATTERS!! 10. What is the function of enzymes in biological systems? Why are they necessary for all biochemical reactions? 11. Explain why e ...
ppt part 1 - Embrace Challenge
ppt part 1 - Embrace Challenge

... • Tris buffer to provide appropriate pH • SDS (sodium dodecyl sulfate) detergent to dissolve proteins and give them a negative charge • Glycerol to make samples sink into wells • Bromophenol Blue dye to visualize samples ...
Supplementary Information
Supplementary Information

... reactions of ~1.5 kb each. Nested RT-PCR was carried out as described above for generating QRT-PCR standards. Instead of cloning, the resulting PCR products were sequenced directly using BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Inc.) and BetterBuffer (Microzone Ltd) according ...
Datasheet - LifeSensors
Datasheet - LifeSensors

... Interferon stimulated gene 15 (ISG15) is a member of the ubiquitin-like protein family whose expression is increased following stimulation with type 1 Interferons. ISG15-VME is synthesized by the conjugation of 4-amino-but-2-enoic acid methyl ester to the C-terminus of ISG15G156. Binding of ISG15-V ...
were performed essentially as described previously (Witt et al
were performed essentially as described previously (Witt et al

... binding to nebulin, we used a SPOTS blot membrane (JPT, Berlin) that displays exon 4 – exon 7 of titin (see also EMBL data library, accession AJ277892) as a series of 31 overlapping residues (peptides were acetylated at their amino terminus, to enhance stability). Initially, the membrane was washed ...
Genes and DNA2012
Genes and DNA2012

... 3. Proteins make up parts such as hair, horns, hooves, nails, ...
doc - University of California, Santa Cruz
doc - University of California, Santa Cruz

... University of California Santa Cruz ...
DNA Notes
DNA Notes

... * Amino acids bond to each other to form protiens ...
Biological Macromolecules and Lipids
Biological Macromolecules and Lipids

... 2 The cap attaches, causing 3 The cap comes the cylinder to change off, and the shape in such a way that properly folded it creates a hydrophilic protein is environment for the released. folding of the polypeptide. ...
About
About

... Matthews Correlation Coefficient (MCC) = 0.425 ...
Communication - Dundee Life Sciences
Communication - Dundee Life Sciences

... the translocation of tightly folded proteins across a spectrum of biological membranes. ...
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Gene expression



Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.
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