Three functionally diverged major structural proteins of white spot
... A 133 bp fragment was obtained and, after purification from a 2 % agarose gel, cloned into pBluescript SK(j) and sequenced. The sequence of this PCR product corresponded with the Nterminal protein sequence of WSSV VP24 and was used as probe in a colony lift assay (Sambrook et al., 1989) on WSSV plas ...
... A 133 bp fragment was obtained and, after purification from a 2 % agarose gel, cloned into pBluescript SK(j) and sequenced. The sequence of this PCR product corresponded with the Nterminal protein sequence of WSSV VP24 and was used as probe in a colony lift assay (Sambrook et al., 1989) on WSSV plas ...
5. CH 5 PPT The Structure and Function of Macromolecules
... reactions called dehydration reactions • Polymers are disassembled to monomers by hydrolysis, a reaction that is essentially the reverse of the dehydration reaction ...
... reactions called dehydration reactions • Polymers are disassembled to monomers by hydrolysis, a reaction that is essentially the reverse of the dehydration reaction ...
Slide 1
... DNA carries information from one generation to the next DNA determines the heritable characteristics of organisms DNA is easily copied ...
... DNA carries information from one generation to the next DNA determines the heritable characteristics of organisms DNA is easily copied ...
Document
... • Different evolutionary origins, no sequence similarity • Similarities in the reaction mechanisms. Chymotrypsin, subtilisin and carboxypeptidase C have a catalytic triad of serine, aspartate and histidine in common: serine acts as a nucleophile, aspartate as an electrophile, and histidine as a base ...
... • Different evolutionary origins, no sequence similarity • Similarities in the reaction mechanisms. Chymotrypsin, subtilisin and carboxypeptidase C have a catalytic triad of serine, aspartate and histidine in common: serine acts as a nucleophile, aspartate as an electrophile, and histidine as a base ...
Document
... strands by heating them to 95oC for 30s -1 min • Annealing – primers bind to target sequence by cooling reaction to 40-60oC for 30s - 1 min • Extension – Taq Polymerase extends the primers and copies each DNA template strand by heating to 72oC for 30s - 1 min ...
... strands by heating them to 95oC for 30s -1 min • Annealing – primers bind to target sequence by cooling reaction to 40-60oC for 30s - 1 min • Extension – Taq Polymerase extends the primers and copies each DNA template strand by heating to 72oC for 30s - 1 min ...
transcription/translation game
... DNA transcription to messenger RNA (mRNA) and its subsequent translation into an amino acid polymer (peptide or protein). DNA is transcribed into mRNA using the A-T/G-C pairing (but substituting uracil (U) for thiamine (T)). The mRNA is then translated into a peptide sequence using transfer RNA (tRN ...
... DNA transcription to messenger RNA (mRNA) and its subsequent translation into an amino acid polymer (peptide or protein). DNA is transcribed into mRNA using the A-T/G-C pairing (but substituting uracil (U) for thiamine (T)). The mRNA is then translated into a peptide sequence using transfer RNA (tRN ...
Q1. Lysozyme is an enzyme consisting of a single polypeptide chain
... Explain why the percentages of bases from the middle part of the chromosome and the end part are different. ...
... Explain why the percentages of bases from the middle part of the chromosome and the end part are different. ...
Spnr, a Murine RNA-binding Protein That Is Localized to
... nucleic acid sequence and the deduced amino acid sequence are shown in Fig. 1 a. The Spnr cDNA codes for a 649-amino acid protein of 71 kD that has significant homology with a family of known RNA-binding proteins that includes Xenopus 4F-1 and 4F-2, Drosophila staufen, human Tar-binding protein and ...
... nucleic acid sequence and the deduced amino acid sequence are shown in Fig. 1 a. The Spnr cDNA codes for a 649-amino acid protein of 71 kD that has significant homology with a family of known RNA-binding proteins that includes Xenopus 4F-1 and 4F-2, Drosophila staufen, human Tar-binding protein and ...
Stanley Miller`s Experiment
... conclusion. “But,” cautions Lazcano, “as more and more completely sequenced cellular genomes have become available, their analysis has shown that an extensive amount of horizontal transfer of genes occurred, leading many to wonder whether we will ever untangle the weblike phylogenies of early cell e ...
... conclusion. “But,” cautions Lazcano, “as more and more completely sequenced cellular genomes have become available, their analysis has shown that an extensive amount of horizontal transfer of genes occurred, leading many to wonder whether we will ever untangle the weblike phylogenies of early cell e ...
The Localization of PABPC1 in HeLa Cells
... Once the mRNA has been processed in the nucleus, it is then allowed to pass into the cytoplasm. A complex in the nuclear pore is able to recognize which mRNA molecules are correct and allows them to pass through into the cytoplasm. Once in the cytoplasm, the building blocks of the mRNA or nucleotide ...
... Once the mRNA has been processed in the nucleus, it is then allowed to pass into the cytoplasm. A complex in the nuclear pore is able to recognize which mRNA molecules are correct and allows them to pass through into the cytoplasm. Once in the cytoplasm, the building blocks of the mRNA or nucleotide ...
Adobe PDF - CL Davis Foundation
... process by which they inactivate (C) (C). Transitions from the open to the inactivated state reduce the number of channels that are available to conduct ions. Mutations that destabilize the inactivated, nonconducting state of the channel are gain-of-function mutations and are common to diverse disea ...
... process by which they inactivate (C) (C). Transitions from the open to the inactivated state reduce the number of channels that are available to conduct ions. Mutations that destabilize the inactivated, nonconducting state of the channel are gain-of-function mutations and are common to diverse disea ...
Getting the most out of milk
... Proteins have several levels of structure. The amino acid sequence forms the primary protein structure. When the amino acid sequences become linked, they form either sheets or helixes called the secondary structure. These secondary structures interact to form globular 3D shapes called the tertiary s ...
... Proteins have several levels of structure. The amino acid sequence forms the primary protein structure. When the amino acid sequences become linked, they form either sheets or helixes called the secondary structure. These secondary structures interact to form globular 3D shapes called the tertiary s ...
Interaction of cycloheximide with 25S ribosomal RNA from yeast
... cerevisiae are inhibited by cycloheximide. This yeast possesses a ribosomal protein L41 that has a different primary structure from that of its counterpart in K. lact i s . The above observations suggest that the ribosomal domain that is, in S.cerevisiae, involved with the interaction of cyclEheximi ...
... cerevisiae are inhibited by cycloheximide. This yeast possesses a ribosomal protein L41 that has a different primary structure from that of its counterpart in K. lact i s . The above observations suggest that the ribosomal domain that is, in S.cerevisiae, involved with the interaction of cyclEheximi ...
Gene Section JUNB (jun B proto-oncogene) Atlas of Genetics and Cytogenetics
... JUNB is a member of JUN family (JUN, JUNB and JUND) that can dimerize with one another, or with members of Fos and ATF families, to form AP-1 transcription factor. Comparing with JUN, the transactivation activity of JUNB is much weaker. Due to the small differences on the amino acid sequences in the ...
... JUNB is a member of JUN family (JUN, JUNB and JUND) that can dimerize with one another, or with members of Fos and ATF families, to form AP-1 transcription factor. Comparing with JUN, the transactivation activity of JUNB is much weaker. Due to the small differences on the amino acid sequences in the ...
ppt
... Genetic information DNA → RNA → Protein • RNA polymerase synthesizes RNA from DNA templates (transcription): ...
... Genetic information DNA → RNA → Protein • RNA polymerase synthesizes RNA from DNA templates (transcription): ...
Transcription – Gene regulation
... mapping of the TBP. The blue mesh corresponds to the holo-TFIID, with the A, B, and C lobes indicated. (A) The green mesh corresponds to the density difference between the holo-TFIID and the TFIID-IIB complex. (B) The magenta and green meshes show the density difference between the holo-TFIID and th ...
... mapping of the TBP. The blue mesh corresponds to the holo-TFIID, with the A, B, and C lobes indicated. (A) The green mesh corresponds to the density difference between the holo-TFIID and the TFIID-IIB complex. (B) The magenta and green meshes show the density difference between the holo-TFIID and th ...
Multipower Sportsfood launches Fit Protein Lite
... Fit Protein Lite delivers 80% less carbs and sugars than Multipower’s number one selling Fit Protein in the iconic brown bottle. Retailing at just £3.85 a bottle the 500ml drink is available in three delicious flavours of Chocolate, Vanilla and Strawberry. Multipower Nutritionist Drew Price said: “F ...
... Fit Protein Lite delivers 80% less carbs and sugars than Multipower’s number one selling Fit Protein in the iconic brown bottle. Retailing at just £3.85 a bottle the 500ml drink is available in three delicious flavours of Chocolate, Vanilla and Strawberry. Multipower Nutritionist Drew Price said: “F ...
CH 6: Proteins and Amino Acids
... a regular/daily basis for proteins to be made – Need all 20 a.a. to make most proteins – Animal sources of proteins contain all 9 essential aa (one exception, gelatin) – Plant sources are missing or low in at least one essential aa (one exception, soy) ...
... a regular/daily basis for proteins to be made – Need all 20 a.a. to make most proteins – Animal sources of proteins contain all 9 essential aa (one exception, gelatin) – Plant sources are missing or low in at least one essential aa (one exception, soy) ...
regulation of a bacteriophage t4 late gene, soc, which
... A short (approximately 300-base) transcript (marked with a closed square in Figure 3), detected with probe 2 but not with probe 1, was present only in the, late RNA samples (Figure 3, panel 2, lane B). The size of this late RNA and its hybridization pattern are consistent with initiation of transcri ...
... A short (approximately 300-base) transcript (marked with a closed square in Figure 3), detected with probe 2 but not with probe 1, was present only in the, late RNA samples (Figure 3, panel 2, lane B). The size of this late RNA and its hybridization pattern are consistent with initiation of transcri ...
15-25 kD
... – Class – Order – Family – Genus – Species • Traditional classification based upon traits: – Morphological – Behavioral ...
... – Class – Order – Family – Genus – Species • Traditional classification based upon traits: – Morphological – Behavioral ...
CH 5
... almost everything that an organism does. Protein functions include structural support, storage, transport, cellular signaling, movement, and defense against foreign substances. Most important, protein enzymes function as catalysts in cells, regulating metabolism by selectively accelerating chemi ...
... almost everything that an organism does. Protein functions include structural support, storage, transport, cellular signaling, movement, and defense against foreign substances. Most important, protein enzymes function as catalysts in cells, regulating metabolism by selectively accelerating chemi ...
BIOCHEMISTRY, CELL AND MOLECULAR BIOLOGY TEST Time—170 minutes
... patient antibodies against viral proteins. If antibodies against the viral proteins are present, then this is taken as evidence that the patient is infected with the virus. However, a flaw in this method of detection is the existence of a "latent period", i.e., a time period after infection with the ...
... patient antibodies against viral proteins. If antibodies against the viral proteins are present, then this is taken as evidence that the patient is infected with the virus. However, a flaw in this method of detection is the existence of a "latent period", i.e., a time period after infection with the ...
Molecular Cloning
... (i) EM of bacteriophage λ virion. (ii) Simplified view of bacteriophage genome:about 60 genes, replaceable region not essential and can be replaced by foreign DNA up to 25 kb. Large segment of the 48kb DNA of the λ phage are not essential for productive infection and can be replaced with inserts. ( ...
... (i) EM of bacteriophage λ virion. (ii) Simplified view of bacteriophage genome:about 60 genes, replaceable region not essential and can be replaced by foreign DNA up to 25 kb. Large segment of the 48kb DNA of the λ phage are not essential for productive infection and can be replaced with inserts. ( ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.