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molecular biology
molecular biology

... – RNA nucleotides contain ribose while DNA contains deoxyribose (a type of ribose that lacks one oxygen atom) – in RNA the nucleotide uracil substitutes for thymine, which is present in DNA Machine Learning & Bioinformatics ...
Chapter 09 Lecture PowerPoint - McGraw Hill Higher Education
Chapter 09 Lecture PowerPoint - McGraw Hill Higher Education

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Biological sequence analysis
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... RNA occurs in multiple forms (can be double helix but not necessary) and copies; Messenger RNA codes template for protein synthesis; Ribosomal RNA constitute the catalytic core of the ribosome; Transfer RNA is the adaptor between nucleic acids and proteins; Small nuclear RNA are essential component ...
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PowerPoint 演示文稿

... aminoacyl-tRNAs to bind to their codons on mRNA. small subunit of the ribosome is bound to mRNA large subunit carries the nascent polypeptide. A ribosome moves along mRNA from an initiation site in the 5′ region to a termination site in the 3′ region, and the appropriate aminoacyl-tRNAs respond to t ...
Chapter 9 – DNA-Based Information Technologies
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... specific recognition sequence • Type II - cleave double-stranded DNA only, at or near an unmethylated recognition sequence • More than 200 type I and type II are known • Most recognize “palindromic sequences” (read the same in either direction) ...
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... A form is generally observed in partially dehydrated samples of DNA while in the cell it may be produced in hybrid pairings of DNA and RNA strands as well as in enzyme – DNA duplex complexes. DNA is still arranged in right handed double helix but the helix is wider and the number of base pairs per h ...
Biochem 4 protein notes - The Bronx High School of Science
Biochem 4 protein notes - The Bronx High School of Science

... Since shape is determined by amino acid sequence; changing sequence changes 3D shape EX: Sickle cell anemia mutation changes one amino acid in the sequence (glu → ala) Abnormal hemoglobin molecules crystallize; cause blood cells to become sickle shaped FACTORS AFFECTING CONFORMATION Folding occurs a ...
Protein Expression - New England Biolabs
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Gene predictions: structural, discovery, functional part 1
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... prokaryotes where all long ORFs encode genes Genomic repeats Non-canonical (ATG) start codon Splicing (exons & introns) - alternative splicing (40-50% genes) Pseudogenes Long genes or short genes Long introns Non-canonical introns UTR introns Overlapping genes on opposite strands Nested genes overla ...
the genetic code - Blue Valley Schools
the genetic code - Blue Valley Schools

... spaced plus and minus mutants are more likely to be non-functional? How did they restore such genes activity? 9. How do mutations suggest that genes are true and separate entities? 10. What happened when Crick and colleagues created T4 recombinants with three plus mutants fairly close together? How ...
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... (Dicer) to produce short double-stranded siRNA molecules. Argonaute proteins are then required to bind siRNA molecules to form a complex known as RISC (RNA-induced silencing complex). RISCs may then promote epigentic silencing through RNA-directed DNA methylation or by target RNA cleavage. Though pr ...
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Transfer RNA and Protein Building Name_________________

... important molecules used for: building cell parts, as transport molecules, as enzymes and hormones and numerous other functions. Proteins are built of long chains of ______________________________. Each protein must be built with the correct sequence of amino acids. How does mRNA direct the ribosome ...
Chem 4B Final Exam Review Sheet Systematic error
Chem 4B Final Exam Review Sheet Systematic error

... 95 C: This temperature \melts" the double stranded template DNA to form ssDNA, thus exposing the base pair sequence to solution and making them accessible to the primers. DNA polymerase does not synthesize DNA at this stage because there are no paired strands. 58 C: The solution is now cool enough t ...
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Protein engineering: navigating between chance and reason

... Georgiou at the University of Texas, Austin have pioneered techniques in which cells producing proteins with desirable properties can be easily sorted by flow cytometry using fluorescence-activated cell sorting. Still, getting display technologies to select for properties beside binding is often imp ...
Exam Review 2 10/2/16
Exam Review 2 10/2/16

... A. You have ADH 3 heterozygote B. You have ALDH 2 homozygous 1 C. You have ADH3 homozygous 1 D. You have ADH 2 homozygous 1 53. If oxygen in cellular respiration was labeled with oxygen isotopes, what molecule in the cell would we look for to have the isotope? A. Glucose B. Water C. CO2 D. ATP 54. I ...
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Gene expression



Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.
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