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Genome-wide Dissections of DNA Damage Induced Transcriptional
Genome-wide Dissections of DNA Damage Induced Transcriptional

... • Partition the responding genes into distinct sets, each with a particular expression pattern  Identify major patterns in the data: reduce the dimensionality of the problem  co-expression → co-function  co-expression → co-regulation • Partition the genes to achieve:  Homogeneity: genes inside a ...
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... target protein is engineered to transcribe an ACA-less mRNA, its transcript is stably co-expressed and translated in MazFinduced cells (2). Codon degeneracy enables alteration of an ACA triplet to a cleavage-resistant sequence without changing the protein amino acid sequence, regardless of its posit ...
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Gene expression



Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.
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