Chapter 47
... 1. The restriction enzyme, HindIII recognizes the sequence 5’-AAGCTT-3’, cutting between the two A’s on both strands. Draw the double-stranded sequence before and after the enzyme cuts. What type of bonds are being cleaved by the restriction enzyme? (Cues: active site, complementary shape, phosphate ...
... 1. The restriction enzyme, HindIII recognizes the sequence 5’-AAGCTT-3’, cutting between the two A’s on both strands. Draw the double-stranded sequence before and after the enzyme cuts. What type of bonds are being cleaved by the restriction enzyme? (Cues: active site, complementary shape, phosphate ...
DNA- Experiments and People
... EXPERIMENT: MATTHEW MESELSON AND FRANKLIN STAHL- 1958 Grow E. coli bacteria with radioactive 15N (its heavier than 14N) so bacteria incorporate heavy N into their DNA Then grow in media with only 14N Centrifuge DNA at different times to separate by size. (The more 15N it has the heavier it is) ...
... EXPERIMENT: MATTHEW MESELSON AND FRANKLIN STAHL- 1958 Grow E. coli bacteria with radioactive 15N (its heavier than 14N) so bacteria incorporate heavy N into their DNA Then grow in media with only 14N Centrifuge DNA at different times to separate by size. (The more 15N it has the heavier it is) ...
DNA People - Biology Junction
... EXPERIMENT: MATTHEW MESELSON AND FRANKLIN STAHL- 1958 Grow E. coli bacteria with radioactive 15N (its heavier than 14N) so bacteria incorporate heavy N into their DNA Then grow in media with only 14N Centrifuge DNA at different times to separate by size. (The more 15N it has the heavier it is) ...
... EXPERIMENT: MATTHEW MESELSON AND FRANKLIN STAHL- 1958 Grow E. coli bacteria with radioactive 15N (its heavier than 14N) so bacteria incorporate heavy N into their DNA Then grow in media with only 14N Centrifuge DNA at different times to separate by size. (The more 15N it has the heavier it is) ...
dna replication
... Ends of eukaryotic chromosomes, the telomeres, have special nucleotide sequences Humans - this sequence is typically TTAGGG, repeated 100 - 1,000 times (not an actual used gene) Telomerase adds a short molecule of RNA as a template to extend the 3’ end Now there is room for primase & DNA pol to exte ...
... Ends of eukaryotic chromosomes, the telomeres, have special nucleotide sequences Humans - this sequence is typically TTAGGG, repeated 100 - 1,000 times (not an actual used gene) Telomerase adds a short molecule of RNA as a template to extend the 3’ end Now there is room for primase & DNA pol to exte ...
Review 16-18
... The final stage of tsln is termination the ribosome reaches a stop codon in the mRNA The pp may undergo further modification B4 it becomes a ...
... The final stage of tsln is termination the ribosome reaches a stop codon in the mRNA The pp may undergo further modification B4 it becomes a ...
Break it down, DNA song
... The DNA, the DNA, the DNA makes protein Transcription takes the bases that are found in one gene Converts them to RNA if you know what I mean The bases pair up, just like they did before, But U subs for T which isn’t needed anymore RNA leaves the nucleus but the job isn’t done Ribosomes roll in to j ...
... The DNA, the DNA, the DNA makes protein Transcription takes the bases that are found in one gene Converts them to RNA if you know what I mean The bases pair up, just like they did before, But U subs for T which isn’t needed anymore RNA leaves the nucleus but the job isn’t done Ribosomes roll in to j ...
Ch 12 Lecture Notes - PPT
... manufactured, it occurs in the nucleus •Enzyme, RNA polymerase binds to a strand of DNA, causing bonds between nitrogen bases to break and separates strands •Individual mRNA nucleotides floating around in the nucleus bind with one unraveled DNA strand •Uses DNA strand as a template to create the RNA ...
... manufactured, it occurs in the nucleus •Enzyme, RNA polymerase binds to a strand of DNA, causing bonds between nitrogen bases to break and separates strands •Individual mRNA nucleotides floating around in the nucleus bind with one unraveled DNA strand •Uses DNA strand as a template to create the RNA ...
History of Genetics
... REVIEW • Where in the cell are chromosomes located? Nucleus • How many pairs of chromosomes do we as Humans have? ...
... REVIEW • Where in the cell are chromosomes located? Nucleus • How many pairs of chromosomes do we as Humans have? ...
Gene Expression - OpotikiCollegeBiology
... • Scientists worked out that chromosomes come in pairs, • The pair has the same genes on them, • So you get two versions of each gene, one on the chromosome from the mother and one on the chromosome from the father. • The genes are on matching locations on the pair of chromosomes so they are called ...
... • Scientists worked out that chromosomes come in pairs, • The pair has the same genes on them, • So you get two versions of each gene, one on the chromosome from the mother and one on the chromosome from the father. • The genes are on matching locations on the pair of chromosomes so they are called ...
DNA - The Double Helix - OG
... cell DNA. The new cells then receive the instructions and information needed to function. The process of copying DNA is called replication. Replication in prokaryotes is different than in eukaryotes. Prokaryotes have circular DNA. In this process, replication starts at one point and proceeds in both ...
... cell DNA. The new cells then receive the instructions and information needed to function. The process of copying DNA is called replication. Replication in prokaryotes is different than in eukaryotes. Prokaryotes have circular DNA. In this process, replication starts at one point and proceeds in both ...
lecture notes-molecular biology-web
... The primary product of transcription: m-RNA, t-RNA and r-RNA. - RNA polymerases consist of : sigma factor: a protein locate the beginning for the message. core enzyme: it contains the active sites. Read 3’ to 5’ direction of DNA template RNA is synthesized in the direction of 5’ to 3’. - Both strand ...
... The primary product of transcription: m-RNA, t-RNA and r-RNA. - RNA polymerases consist of : sigma factor: a protein locate the beginning for the message. core enzyme: it contains the active sites. Read 3’ to 5’ direction of DNA template RNA is synthesized in the direction of 5’ to 3’. - Both strand ...
DNA Technology
... This spurred a revolution in biotechnology innovation around the world and played a key role in making the U.S. the global leader in the new ...
... This spurred a revolution in biotechnology innovation around the world and played a key role in making the U.S. the global leader in the new ...
Ch6.1 - Cobb Learning
... 9.Changes in the number, type, or order of DNA bases: 26.How often is DNA copied? ______________________________________________________________________________ 10.Analysis of fragments of DNA as a form of identification (two words): 27.What in the cell helps with unwinding, copying, and rewinding t ...
... 9.Changes in the number, type, or order of DNA bases: 26.How often is DNA copied? ______________________________________________________________________________ 10.Analysis of fragments of DNA as a form of identification (two words): 27.What in the cell helps with unwinding, copying, and rewinding t ...
Protein Synth Mutation test review
... 13. After transcription takes place the __________ leaves the nucleus and travels to a _______________ in the cytoplasm or on the Rough ER. 14. In the cytoplasm, the second phase of protein synthesis called _________________ takes place. ...
... 13. After transcription takes place the __________ leaves the nucleus and travels to a _______________ in the cytoplasm or on the Rough ER. 14. In the cytoplasm, the second phase of protein synthesis called _________________ takes place. ...
Chapter 6 DNA Replication
... strand by joining the first nucleotides. Nucleotides must be added to the end of an already existing chain, called primer. The primer is not a DNA, but short stretch of RNA. Still another enzyme, primase, makes the primer. ...
... strand by joining the first nucleotides. Nucleotides must be added to the end of an already existing chain, called primer. The primer is not a DNA, but short stretch of RNA. Still another enzyme, primase, makes the primer. ...
DNA replication
... The need for a specialized region at the ends of eukaryotic chromosomes is apparent when we consider that all known DNA polymerases elongate DNA chains from the 3′ end, and all require an RNA or DNA primer. As the growing fork approaches the end of a linear chromosome, synthesis of the leading stra ...
... The need for a specialized region at the ends of eukaryotic chromosomes is apparent when we consider that all known DNA polymerases elongate DNA chains from the 3′ end, and all require an RNA or DNA primer. As the growing fork approaches the end of a linear chromosome, synthesis of the leading stra ...
Protein Synthesis Notes
... tRNA (compliment of mRNA) picks up specific amino acids from the cytoplasm and attaches to the mRNA strand. ...
... tRNA (compliment of mRNA) picks up specific amino acids from the cytoplasm and attaches to the mRNA strand. ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.