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DNA and genetic information
DNA and genetic information

... The genetic code is universal • This fact indicates a single origin for all ...
sequence
sequence

Biology Final Jeopary 1
Biology Final Jeopary 1

... A: The molecule in a cell responsible for storing and transmitting genetic information (info for making proteins); has the shape of a double-helix. ...
Introduction to Genetics
Introduction to Genetics

... nucleosomes – Humans contain 46 such molecules (23 pairs) • 44 somatic chromosomes • 2 sex chromosomes (X +Y) ...
DNA, Chromosomes & Genes - Blountstown Middle School
DNA, Chromosomes & Genes - Blountstown Middle School

... http://ghr.nlm.nih.gov/info=basics/show/gene ...
Assessment Builder - Printer Friendly Version Name: Date: 1 The
Assessment Builder - Printer Friendly Version Name: Date: 1 The

... One possible way for humans to produce species A plants with the ability to synthesize this protein would be to (1) mutate fungal DNA and introduce the mutated DNA into species B using a virus (2) add DNA from species B into the soil around species A (3) insert the gene for the protein from species ...
Gene Regulation
Gene Regulation

... – Build up larger molecules – Once end product is present pathway turns off ...
Biotechnology Part 3 Outline
Biotechnology Part 3 Outline

... A. The first step in this process uses restriction enzymes to create “Sticky Ends” on a plasmid and DNA from another source. 1. These are enzymes that cut DNA at specific nucleotide sequences. a. This specific DNA sequence is referred to as the restriction site. 2. These enzymes create restriction f ...
6 Day 9 Biotechnology Part 3 Outline
6 Day 9 Biotechnology Part 3 Outline

... A. The first step in this process uses restriction enzymes to create “Sticky Ends” on a plasmid and DNA from another source. 1. These are enzymes that cut DNA at specific nucleotide sequences. a. This specific DNA sequence is referred to as the restriction site. 2. These enzymes create restriction f ...
Final Exam Study Guide Ms. Thomas Spring 2011
Final Exam Study Guide Ms. Thomas Spring 2011

... 13. Draw and label a DNA nucleotide. 14. Draw and label the major parts of a flower. Describe the function of each part. 15. Draw and label the stages of meiosis. 16. Draw the following cycles and define each process within the cycle: a. Water b. Carbon c. Nitrogen 17. How many chromosomes are prese ...
DNA Replication
DNA Replication

... Forks (Y-shaped region) ...
Answers chapter 9
Answers chapter 9

... the DNA by the endonucleolytic cleavage is repaired by DNA polymerase (using the undamaged strand as a template) and DNA ligase. If the excision repair system fails to detect a damaged base prior to replication, a backup mechanism exists that can intervene before a point mutation is permanently esta ...
Slide 1
Slide 1

... Both orientations of insert DNA possible. Tandem copies of insert possible. Restriction sites at junctions often eliminated. Tandem copies of insert DNA possible. Both orientations possible. Restriction sites at junctions preserved. Background of non-recombinants is low. One possible orientation of ...
Concept 20.1 A. -Plasmid is the cloning vector.
Concept 20.1 A. -Plasmid is the cloning vector.

... - Expression of a Eukaryotic gene in a Prokaryote may be difficult because of a) Different aspects of gene expression: - To overcome difficulties in promoters, and other control sequences we use an expression vector. - This vector contains a very active prokaryotic promoter just upstream of a restri ...
Complete DNA Function Vocab with definitions
Complete DNA Function Vocab with definitions

... A single linear strand of DNA (and associated structural proteins) that carries the genes and functions in the transmission of hereditary information The backbone of nucleic acid constructed from alternating ribose sugar and phosphate molecules. a part of the cell containing DNA and RNA and responsi ...
Manipulating DNA
Manipulating DNA

... cells" — cells that, in theory, can ultimately grow into any kind of cell in the body. • These cells could be used to generate new organs or cell clusters to treat patients with failing organs or degenerative diseases ...
B left E
B left E

... Right strand, leading strand sysnthesis, reading the template 3’ to 5’ Okazaki fragments on the right The beta clamp is involved in DNA synthesis on both the left and right supercoiling the DNA duplex occurs near E Single strand binding proteins promote duplex (double stranded) DNA ...
Review Questions
Review Questions

... The same goes for guanine and cytosine. Adenine and guanine are both purines (nitrogen bases composed of double rings). Cytosine and thymine each are single-ring bases so they are pyrimidines. You may notice that in each complementary pair of bases there is one purine and one pyrimidine. This arrang ...
DNA Structure: Deoxyribonucleic acid
DNA Structure: Deoxyribonucleic acid

Genetic Engineering pp 2014
Genetic Engineering pp 2014

... 1. Cut human insulin gene with restriction enzymes. 2. Cut the bacterial plasmid (chromosome) with the same restriction enzymes. 3. Combine the human insulin gene, bacterial plasmid, and ligase (an enzyme that helps form the hydrogen bonds) 4. Insert the recombinant plasmid into a bacteria cell. 5. ...
Protein Synthesis - Doral Academy High School
Protein Synthesis - Doral Academy High School

... • Messenger RNA (mRNA) copies DNA’s code & carries the genetic information to the ribosomes • Ribosomal RNA (rRNA), along with protein, makes up the ribosomes • Transfer RNA (tRNA) transfers amino acids to the ribosomes where proteins are synthesized ...
History_of_DNA
History_of_DNA

... E.Coli DNA polymerase I requires: 1. All four dNTPs (dATP, dGTP, dCTP and dTTP) 2. A primer chain with a free 3`-OH end 3. A template strand to which the primer is basepaired • Double-stranded DNA that is fully intact and lacking a free 3`-OH end will not be replicated (Ex: Intact circular DNA) 4. M ...
Transcription Worksheet
Transcription Worksheet

... DNA replication is the process by which a cell copies its DNA. During replication, both strands of the double helix are used as templates to make complementary, or matching, strands of DNA. DNA transcription is the process by which a single strand of DNA is used as a template to generate a strand of ...
REVIEW OF MOLECULAR GENETICS - Pascack Valley Regional
REVIEW OF MOLECULAR GENETICS - Pascack Valley Regional

... DNA library - a random collection of DNA fragments from an organism cloned into a vector Ideally contains at least one copy of every DNA sequence. Easily maintained in the laboratory Can be manipulated in various ways to facilitate the isolation of a DNA fragment of interest to a scientist. Num ...
What is a genome?
What is a genome?

... •  Process by which DNA copies  itself before cell division  •  DNA Molecule is “unzipped”  between bases by enzymes  called helicases  •  DNA polymerase enzymes  copy each strand to make 2  new iden9cal DNA molecules  •  DNA ligase joins fragments of  DNA together  •  See DNA Replica9on Tutorial  f ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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