Microplate-Based Pathlength Correction Method for Photometric
... correction is performed, the results are no longer dependent on the volume. The microplate results—after pathlength correction—correlate perfectly with the cuvette results. The only exception seen in the figures is a slight variation between the 20 µl assay volume results using 384-well plates and t ...
... correction is performed, the results are no longer dependent on the volume. The microplate results—after pathlength correction—correlate perfectly with the cuvette results. The only exception seen in the figures is a slight variation between the 20 µl assay volume results using 384-well plates and t ...
Distortion of quantitative genomic and expression
... regarding reproducibility of these techniques have been raised by cross-validation studies in different laboratories (1–5). Strategies to mitigate variability in the results obtained from replicate studies have focused on standardizing technical factors, such as array production, RNA synthesis, labe ...
... regarding reproducibility of these techniques have been raised by cross-validation studies in different laboratories (1–5). Strategies to mitigate variability in the results obtained from replicate studies have focused on standardizing technical factors, such as array production, RNA synthesis, labe ...
Molecular cloning and expression of full
... cleaved intopolypeptides of 32kDand 170kD respectively. Theother BRHA encoded polypeptides were shown to be all derived from the 170kD polypeptide (seeFigure 1)and for some of these proteins the function was known. BRNA was shown tobe replicated and expressed independently in cowpea protoplasts indi ...
... cleaved intopolypeptides of 32kDand 170kD respectively. Theother BRHA encoded polypeptides were shown to be all derived from the 170kD polypeptide (seeFigure 1)and for some of these proteins the function was known. BRNA was shown tobe replicated and expressed independently in cowpea protoplasts indi ...
CBSE XII BIO QS with key(2009-2015)
... they have food reserves for the seedling or embryo the seed coat provides protection to the embryo generate genetic variation remain viable for a considerable period of time.(any two) =1x2 State what is apomixis? Comment on its significance. How can it be commercially used? (2015) Form of asexual re ...
... they have food reserves for the seedling or embryo the seed coat provides protection to the embryo generate genetic variation remain viable for a considerable period of time.(any two) =1x2 State what is apomixis? Comment on its significance. How can it be commercially used? (2015) Form of asexual re ...
Characterization of microbial activity in the surface layers of a
... that the abiotic adsorption is constant with time. Studies with adenine showed that this is not strictly the case; however, adsorption was rapid (on the order of seconds) and subsequent increases were slight. 3HDNA measurements, on the other hand, were unaffected by the adsorbed adenine and hence no ...
... that the abiotic adsorption is constant with time. Studies with adenine showed that this is not strictly the case; however, adsorption was rapid (on the order of seconds) and subsequent increases were slight. 3HDNA measurements, on the other hand, were unaffected by the adsorbed adenine and hence no ...
Brand, Veronica - Degenerate Primer Design using Computational Tools
... primers with the highest coverage to the third stage. The third stage is a refinement stage that uses a hill-climbing procedure to incrementally change two positions: one character is removed from a degenerate position and added at a different position as long as coverage of the primer increases (Li ...
... primers with the highest coverage to the third stage. The third stage is a refinement stage that uses a hill-climbing procedure to incrementally change two positions: one character is removed from a degenerate position and added at a different position as long as coverage of the primer increases (Li ...
How Cations Can Assist DNase I in DNA Binding and Hydrolysis
... degradation [20] and its two disulfide bridges from reduction. Equilibrium dialysis and various spectroscopic studies have led to the conclusion that bpDNase I contains two strong and several weak cation-binding sites [21,22]. More precise information on the number and the location of divalent catio ...
... degradation [20] and its two disulfide bridges from reduction. Equilibrium dialysis and various spectroscopic studies have led to the conclusion that bpDNase I contains two strong and several weak cation-binding sites [21,22]. More precise information on the number and the location of divalent catio ...
Inquiry into Life Twelfth Edition
... The Mechanism of CAP Action • CAP-cAMP complex binds to the lac promoter – Mutants whose lac gene is not stimulated by complex had the mutation in the lac promoter – Mapping the DNA has shown that the activator-binding site lies just upstream of the promoter ...
... The Mechanism of CAP Action • CAP-cAMP complex binds to the lac promoter – Mutants whose lac gene is not stimulated by complex had the mutation in the lac promoter – Mapping the DNA has shown that the activator-binding site lies just upstream of the promoter ...
The Role of DNA-PKcs and Artemis in Opening Viral DNA
... DNA was collected from each step in the process of no-end genome synthesis, i.e., for step 1, PvuII was added; step 2, PvuII and Exo III were added; step 3, PvuII, Exo III, and T4 DNA Pol were added; and step 4, PvuII, Exo III, T4 DNA Pol, and T4 DNA Lig were added. A portion of each DNA collection ...
... DNA was collected from each step in the process of no-end genome synthesis, i.e., for step 1, PvuII was added; step 2, PvuII and Exo III were added; step 3, PvuII, Exo III, and T4 DNA Pol were added; and step 4, PvuII, Exo III, T4 DNA Pol, and T4 DNA Lig were added. A portion of each DNA collection ...
Small aminoacyl transfer centers at GU within a larger RNA
... Separate aminoacyl transfer centers related to the small …GUNNN..: NNNU ribozyme seem possible at the frequent GU sequences dispersed throughout an RNA tertiary structure. In fact, such activity is easily detected and varies more than 2 orders in rate, probably being faster at sites with less struct ...
... Separate aminoacyl transfer centers related to the small …GUNNN..: NNNU ribozyme seem possible at the frequent GU sequences dispersed throughout an RNA tertiary structure. In fact, such activity is easily detected and varies more than 2 orders in rate, probably being faster at sites with less struct ...
Transposon stability and a role for conjugational transfer in adaptive mutability
... (16–21). Moreover, even lacI33 Lac⫺ cells, products of conjugal transfer to scavenger, frequently had become TetS. About 10% of the late-appearing Lac⫹ revertants that are TetS have lost the transposon DNA, most often precisely, and an equal number show evidence of amplification of the Lac兾Tn geneti ...
... (16–21). Moreover, even lacI33 Lac⫺ cells, products of conjugal transfer to scavenger, frequently had become TetS. About 10% of the late-appearing Lac⫹ revertants that are TetS have lost the transposon DNA, most often precisely, and an equal number show evidence of amplification of the Lac兾Tn geneti ...
Sequence requirements for function of the
... su(Hw)BS, ACE3, S18 and ori-β was liberated from the BP construct by digestion with NotI and XhoI. The fragment was cloned into the NotI to XhoI sites of pBS*K to generate pBS*K-2.8. A subregion of the 320 bp ACE3 (ACE3mt-1) was amplified by PCR using primer set 1, digested at the KpnI and BamHI sit ...
... su(Hw)BS, ACE3, S18 and ori-β was liberated from the BP construct by digestion with NotI and XhoI. The fragment was cloned into the NotI to XhoI sites of pBS*K to generate pBS*K-2.8. A subregion of the 320 bp ACE3 (ACE3mt-1) was amplified by PCR using primer set 1, digested at the KpnI and BamHI sit ...
Analysis of Cross Sequence Similarities for Multiple - PolyU
... sample sequence while the subsequence ‘ACGCAT’ listed between 7 and 12 is the 7th to 12th bases of the sample sequence. The vertical line located between two bases indicates that the upper base is identical to the lower base. By comparing the first subsequence (1st to 6th bases) and the second subse ...
... sample sequence while the subsequence ‘ACGCAT’ listed between 7 and 12 is the 7th to 12th bases of the sample sequence. The vertical line located between two bases indicates that the upper base is identical to the lower base. By comparing the first subsequence (1st to 6th bases) and the second subse ...
Classification of plant-pathogenic mycoplasma
... Based on Southern hybridization with a DNA fragment of an MLO associated with aster yellows, a differentiation between organisms inducing decline symptoms and those causing floral virescence has been proposed (Kuske et al., 1991). Although several organisms have been differentiated using these metho ...
... Based on Southern hybridization with a DNA fragment of an MLO associated with aster yellows, a differentiation between organisms inducing decline symptoms and those causing floral virescence has been proposed (Kuske et al., 1991). Although several organisms have been differentiated using these metho ...
Specialized Transduction by Bacteriophage P22 in Salmonella typhimurium: Genetic and Physical Structure of the Transducing Genomes and the Prophage Attachment Site.
... chromosome. By examining DNA heteroduplexes in the electron microscope, we found that DNA molecules from P22pro-I and P22pro-3 each contain a substitution which adds length to the composite genome making the intracellular replicated genome too long to fit into a single phage particle. In this respec ...
... chromosome. By examining DNA heteroduplexes in the electron microscope, we found that DNA molecules from P22pro-I and P22pro-3 each contain a substitution which adds length to the composite genome making the intracellular replicated genome too long to fit into a single phage particle. In this respec ...
Drosophila - The Solomon H Snyder Department of Neuroscience
... then performed to amplify the genomic sequence between the transposon insertion and the annealed splinkerette. This is followed by a sequencing reaction with another nested primer. The spPCR reaction remains highly efficient and specific due to the splinkerette design. Since the splinkerette oligonu ...
... then performed to amplify the genomic sequence between the transposon insertion and the annealed splinkerette. This is followed by a sequencing reaction with another nested primer. The spPCR reaction remains highly efficient and specific due to the splinkerette design. Since the splinkerette oligonu ...
Maintenance of genomic integrity by p53: complementary
... result, DNA damage will be repaired during a growth arrest, or the damaged cells will be eliminated, thereby preventing ®xation of DNA damage as mutations. This function of p53 led to the now famous coining of p53 as the `guardian of the genome' by Lane (1992). Although the main features of p53's ro ...
... result, DNA damage will be repaired during a growth arrest, or the damaged cells will be eliminated, thereby preventing ®xation of DNA damage as mutations. This function of p53 led to the now famous coining of p53 as the `guardian of the genome' by Lane (1992). Although the main features of p53's ro ...
ATR Regulates a G2-Phase Cell-Cycle Checkpoint
... only in terms of their viability but also in their degree of sensitivity to g-radiation. In the course of these experiments, we also observed a differential response to HU versus aphidicolin in wild-type cells, with HU primarily inducing a G1 arrest, whereas aphidicolin induced arrest in G2. This st ...
... only in terms of their viability but also in their degree of sensitivity to g-radiation. In the course of these experiments, we also observed a differential response to HU versus aphidicolin in wild-type cells, with HU primarily inducing a G1 arrest, whereas aphidicolin induced arrest in G2. This st ...
Evolutionary dynamics of RNA-like replicator systems
... Interestingly, a similar situation exists in RNA and DNA. RNA and DNA are chemically very similar to each other, the only difference being the presence or absence of one oxygen atom per nucleotide. Although RNA molecules are the only templates from which proteins are translated in the cell, DNA can ...
... Interestingly, a similar situation exists in RNA and DNA. RNA and DNA are chemically very similar to each other, the only difference being the presence or absence of one oxygen atom per nucleotide. Although RNA molecules are the only templates from which proteins are translated in the cell, DNA can ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.