Biotechnology Explorer™ GMO Investigator™ Kit: A - Bio-Rad
... mixed (multiplexed) together but are detected simultaneously, either as bands on an agarose gel or as a SYBR Green I dye signal in this real-time extension. In the description above, primer pairs also include a matched fluorescent probe for each primer set, which allows multiple PCR reactions to be ...
... mixed (multiplexed) together but are detected simultaneously, either as bands on an agarose gel or as a SYBR Green I dye signal in this real-time extension. In the description above, primer pairs also include a matched fluorescent probe for each primer set, which allows multiple PCR reactions to be ...
Microscopic Mechanics of Hairpin DNA Translocation through
... shown that single nucleotide polymorphisms can be detected by measuring the average time required for unzipping a DNA duplex, while Soni and Meller (10) demonstrated the feasibility of an ultrafast sequencing method in which the DNA sequence is read optically by means of fluorescent markers as the d ...
... shown that single nucleotide polymorphisms can be detected by measuring the average time required for unzipping a DNA duplex, while Soni and Meller (10) demonstrated the feasibility of an ultrafast sequencing method in which the DNA sequence is read optically by means of fluorescent markers as the d ...
Biology II Final Exam Practice
... ____ 64. Why is the synthesis stage called this? a. because protein synthesis is taking place b. because DNA synthesis is taking place c. because it combines several smaller stages into one d. because the chromosomes come together ____ 65. Which of these has occurred by the end of prophase? a. Siste ...
... ____ 64. Why is the synthesis stage called this? a. because protein synthesis is taking place b. because DNA synthesis is taking place c. because it combines several smaller stages into one d. because the chromosomes come together ____ 65. Which of these has occurred by the end of prophase? a. Siste ...
Processing the RefSeq and CCDS Annotation Datasets Using the SAS System: Creation of Gene Reference
... two reasons: the computational require of two exclusive IF statements is negligible and indenting made the code more difficult to read on a smaller screen. In either set of IF-THEN-DO-END statements, the approach is the same, but must account for the reverse compliment for the minus-strand. First it ...
... two reasons: the computational require of two exclusive IF statements is negligible and indenting made the code more difficult to read on a smaller screen. In either set of IF-THEN-DO-END statements, the approach is the same, but must account for the reverse compliment for the minus-strand. First it ...
Locked Nucleic Acid (LNA™)
... The affinity-enhancing effect of incorporation of LNA™ monomers into an oligonucleotide is demonstrated by an increase in the duplex melting temperature (Tm) of 2-8 ºC per LNA™ monomer (see table 1). LNA™ oligonucleotides are defined as DNA or RNA nucleotides containing one or more LNA™ nucleosides. ...
... The affinity-enhancing effect of incorporation of LNA™ monomers into an oligonucleotide is demonstrated by an increase in the duplex melting temperature (Tm) of 2-8 ºC per LNA™ monomer (see table 1). LNA™ oligonucleotides are defined as DNA or RNA nucleotides containing one or more LNA™ nucleosides. ...
AraC Protein, Regulation of the L-arabinose Operon in Escherichia
... protein reduces the tumbling rate of the DNA. This can then be detected as an increase in the fluorescence anisotropy. In practice, the situation is slightly more complicated. The most convenient means of attaching a fluorophore to DNA utilizes six-carbon linkers. Rotation about these six bonds subs ...
... protein reduces the tumbling rate of the DNA. This can then be detected as an increase in the fluorescence anisotropy. In practice, the situation is slightly more complicated. The most convenient means of attaching a fluorophore to DNA utilizes six-carbon linkers. Rotation about these six bonds subs ...
53 - Lab Times
... be in danger of extinction when it comes to sequencing entire genomes like there’s no tomorrow. Instead of cloning single genes, as in the “old days”, many of today’s molecular biologists clone large sets of genes to determine their function. ...
... be in danger of extinction when it comes to sequencing entire genomes like there’s no tomorrow. Instead of cloning single genes, as in the “old days”, many of today’s molecular biologists clone large sets of genes to determine their function. ...
HP1 binding to native chromatin in vitro is determined by the hinge
... from its mammalian homologues, whereas xHP1g is highly conserved. Functionally, xHP1a behaves identically to human HP1a. We observe unexpected differences between the two HP1 variants in binding native soluble chromatin, which seem to correlate with their distinct nuclear distributions in vivo. A su ...
... from its mammalian homologues, whereas xHP1g is highly conserved. Functionally, xHP1a behaves identically to human HP1a. We observe unexpected differences between the two HP1 variants in binding native soluble chromatin, which seem to correlate with their distinct nuclear distributions in vivo. A su ...
Formosa haliotis sp. nov., a brown-alga
... representatives of a novel species of the genus Formosa based on a polyphasic characterization. Isolates LMG 28520T and LMG 28521 were obtained from the gut of the abalone Haliotis gigantea collected at an abalone hatchery (Owase, Mie, Japan) in July 2009. Isolates LMG 28522 and LMG 28523 were obtai ...
... representatives of a novel species of the genus Formosa based on a polyphasic characterization. Isolates LMG 28520T and LMG 28521 were obtained from the gut of the abalone Haliotis gigantea collected at an abalone hatchery (Owase, Mie, Japan) in July 2009. Isolates LMG 28522 and LMG 28523 were obtai ...
Detection of chromosome 2 and chromosome 7 within X-ray
... tested to find out the one that gave the best basis for a good signal after whole chromosome painting. Ah" fixation protocols which included the use of acetic acid led to a chromosome paint of minor quality, whereas the use of absolute methanol (-20°C) after cytocentrifugation of the cells onto slid ...
... tested to find out the one that gave the best basis for a good signal after whole chromosome painting. Ah" fixation protocols which included the use of acetic acid led to a chromosome paint of minor quality, whereas the use of absolute methanol (-20°C) after cytocentrifugation of the cells onto slid ...
the hydrophilic, protease-sensitive terminal domains of eucaryotic
... eucaryotic topoisomerase II. While the bacterial and eucaryotic topoisomerase II (topo II) share significant sequence identity (4) and similarity in their threedimensional structures (5,6), they differ in their number of subunits. Bacterial gyrase and topo IV are heterotypic tetramers, gyrA2gyrB2 an ...
... eucaryotic topoisomerase II. While the bacterial and eucaryotic topoisomerase II (topo II) share significant sequence identity (4) and similarity in their threedimensional structures (5,6), they differ in their number of subunits. Bacterial gyrase and topo IV are heterotypic tetramers, gyrA2gyrB2 an ...
Molecular mechanics of the interactions of spermine with DNA: DNA
... the three central torsion angles are 180°, which forces the central diaminobutane moiety into a fully extended conformation and produces a single distance (approximately 6.3 A) between the secondary amino groups (data not shown). The aminopropyl groups attached to the secondary amino groups assume a ...
... the three central torsion angles are 180°, which forces the central diaminobutane moiety into a fully extended conformation and produces a single distance (approximately 6.3 A) between the secondary amino groups (data not shown). The aminopropyl groups attached to the secondary amino groups assume a ...
Etude du régime alimentaire des carnivores par des techniques
... biodiversity. Thus, precise knowledge of their diet is a prerequisite for designing conservation strategies of these endangered species. Direct and indirect monitoring as well as invasive and non-invasive approaches that have been used to study the diet are either biased or have a low resolution. Th ...
... biodiversity. Thus, precise knowledge of their diet is a prerequisite for designing conservation strategies of these endangered species. Direct and indirect monitoring as well as invasive and non-invasive approaches that have been used to study the diet are either biased or have a low resolution. Th ...
Document
... of all cells in all living organisms DNA controls all the chemical changes which take place in cells The kind of cell which is formed, (muscle, blood, nerve etc) is controlled by DNA The kind of organism which is produced (buttercup, giraffe, herring, human etc) is controlled by DNA ...
... of all cells in all living organisms DNA controls all the chemical changes which take place in cells The kind of cell which is formed, (muscle, blood, nerve etc) is controlled by DNA The kind of organism which is produced (buttercup, giraffe, herring, human etc) is controlled by DNA ...
PO 4
... of all cells in all living organisms DNA controls all the chemical changes which take place in cells The kind of cell which is formed, (muscle, blood, nerve etc) is controlled by DNA The kind of organism which is produced (buttercup, giraffe, herring, human etc) is controlled by DNA ...
... of all cells in all living organisms DNA controls all the chemical changes which take place in cells The kind of cell which is formed, (muscle, blood, nerve etc) is controlled by DNA The kind of organism which is produced (buttercup, giraffe, herring, human etc) is controlled by DNA ...
DNA . ppt - biology
... of all cells in all living organisms DNA controls all the chemical changes which take place in cells The kind of cell which is formed, (muscle, blood, nerve etc) is controlled by DNA The kind of organism which is produced (buttercup, giraffe, herring, human etc) is controlled by DNA ...
... of all cells in all living organisms DNA controls all the chemical changes which take place in cells The kind of cell which is formed, (muscle, blood, nerve etc) is controlled by DNA The kind of organism which is produced (buttercup, giraffe, herring, human etc) is controlled by DNA ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.