Supplementary Materials and methods (doc 154K)
... 50% PAO1-GFP. Competitions starting at 50% of each strain maximize the ability to detect small differences in fitness. The exact initial proportions were confirmed via flow cytometry (see conditions below). Mixtures were diluted 200-fold in fresh LB and competed for 16 hours at 37°C with no agitatio ...
... 50% PAO1-GFP. Competitions starting at 50% of each strain maximize the ability to detect small differences in fitness. The exact initial proportions were confirmed via flow cytometry (see conditions below). Mixtures were diluted 200-fold in fresh LB and competed for 16 hours at 37°C with no agitatio ...
ARTICLES - Weizmann Institute of Science
... Eukaryotic genomes are packaged into nucleosome particles that occlude the DNA from interacting with most DNA binding proteins. Nucleosomes have higher affinity for particular DNA sequences, reflecting the ability of the sequence to bend sharply, as required by the nucleosome structure. However, it ...
... Eukaryotic genomes are packaged into nucleosome particles that occlude the DNA from interacting with most DNA binding proteins. Nucleosomes have higher affinity for particular DNA sequences, reflecting the ability of the sequence to bend sharply, as required by the nucleosome structure. However, it ...
What percentage of students have a dominant learning style
... What is a silent mutation? Mutation that does ...
... What is a silent mutation? Mutation that does ...
TAP Express® Kit
... * Terminator mix A is generally recommended since it is more robust with the second PCR reaction, resulting in higher yield and cleaner product. However, the TAP fragments generated using mix A are not compatible with TAP Express Cloning Kit (Cat. No. TAPC2010). In addition, in our hands, the TAP fr ...
... * Terminator mix A is generally recommended since it is more robust with the second PCR reaction, resulting in higher yield and cleaner product. However, the TAP fragments generated using mix A are not compatible with TAP Express Cloning Kit (Cat. No. TAPC2010). In addition, in our hands, the TAP fr ...
Dark matter, Quantum Gravity and Prebiotic Evolution Contents
... Gravimagnetic London field is proportional to the square of Planck constant and the obvious guess is that the replacement h with hgr could explain the enormous discrepancy with GRT if gravimagnetism is in question. This predicts correctly the magnitude of the effect and one also ends up with the ide ...
... Gravimagnetic London field is proportional to the square of Planck constant and the obvious guess is that the replacement h with hgr could explain the enormous discrepancy with GRT if gravimagnetism is in question. This predicts correctly the magnitude of the effect and one also ends up with the ide ...
UCSD_PevznerMolecula.. - Purdue University :: Computer Science
... More Terminology • The genome is an organism’s complete set of DNA. ...
... More Terminology • The genome is an organism’s complete set of DNA. ...
Detection of GM Papaya Event 55-1 in Fresh
... both fresh and processed papaya fruit. GM papaya event 55-1 is a genetically modified organism (GMO) not currently approved for food in Korea. Using a primer set specific to papain, an endogenous papaya gene, we obtained a PCR product only from papaya genomic DNA, thus defining the primer’s specific ...
... both fresh and processed papaya fruit. GM papaya event 55-1 is a genetically modified organism (GMO) not currently approved for food in Korea. Using a primer set specific to papain, an endogenous papaya gene, we obtained a PCR product only from papaya genomic DNA, thus defining the primer’s specific ...
Methods of Human Heredity Study
... haploid number occurs. While "n" normally signifies the gametic or haploid chromosomes number, "2n" is the somatic or diploid chromosome number in ...
... haploid number occurs. While "n" normally signifies the gametic or haploid chromosomes number, "2n" is the somatic or diploid chromosome number in ...
Getting Started With Gel Electrophoresis
... The gel used in electrophoresis is made of a solution of agarose, a polysaccharide obtained from red algae. When dissolved in hot water and allowed to cool, the solution turns into a gel because long agarose molecules hydrogen bond together, creating a large, threedimensional network trapping the wa ...
... The gel used in electrophoresis is made of a solution of agarose, a polysaccharide obtained from red algae. When dissolved in hot water and allowed to cool, the solution turns into a gel because long agarose molecules hydrogen bond together, creating a large, threedimensional network trapping the wa ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.