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EOC Practice Quiz (5) - Duplin County Schools
EOC Practice Quiz (5) - Duplin County Schools

... a. A mutant. b. a hybrid. c. a polyploidy. d. recombinant DNA. 16. A gene that makes it possible to distinguish bacteria that carry a plasmid containing foreign DNA from those that do not is called a (an) a. resistance gene. b. antibiotic. c. genetic marker. d. clone. Objective 3.3.3 17. The human ...
DNA - Corner Canyon Honors Biology
DNA - Corner Canyon Honors Biology

... makes copies of itself to prepare for cell division. ...
Microbial Genomics
Microbial Genomics

THE NUCLEIC ACIDS
THE NUCLEIC ACIDS

... Direction of Replication • The enzyme helicase unwinds several sections of parent DNA • At each open DNA section, called a replication fork, DNA polymerase catalyzes the formation of 5’-3’ester bonds of the leading strand • The lagging strand, which grows in the 3’-5’ direction, is synthesized in s ...
Gene Manipulation-2 - Workforce Solutions
Gene Manipulation-2 - Workforce Solutions

Bacteriophages
Bacteriophages

... ss-circular DNA (size 6407 bp) DNA enter in to cell converted to double stranded molecule known as replicative form or RF. Replicates until there are about 100 copies in the cell. ...
12.1 - DNA History / Discovery
12.1 - DNA History / Discovery

... ● Compacted DNA and proteins = chromosomes ● Genetic information is stored in the nucleus ...
Chapter 17 Guided Notes
Chapter 17 Guided Notes

NOTES: 12.1 - History of DNA (powerpoint)
NOTES: 12.1 - History of DNA (powerpoint)

... ● Compacted DNA and proteins = chromosomes ● Genetic information is stored in the nucleus ● Genetic information is essential; each cell must receive all info. (ensured by MITOSIS) ...
Unit review questions
Unit review questions

... 23. How does the nucleotide sequence in one chain of DNA compare with the other chain of DNA? 24. Why must DNA be able to make copies of itself? 25. Define DNA replication. 26. What is the first step that must occur in DNA replication? 27. What acts as the template in DNA replication? 28. What is a ...
Extracting DNA from Eukayotic Cells
Extracting DNA from Eukayotic Cells

... DNA is a nucleic acid found in the nucleus of cells that stores and transmits genetic information from one generation of an organism to the next by coding for the production of a cell’s proteins. Bacteria, fungi, plants, animals and all other living organisms on this planet contain nucleic acids. Th ...
Document
Document

... • When one nucleotide is replaced with another, it is called a substitution mutation. For example, changing ATCG to ATAG. • When a nucleotide is added into the sequence, it is called an insertion mutation. For example, changing ATCG to ATGCG. • When a nucleotide is lost from the DNA sequence, it is ...
restriction enzyme
restriction enzyme

... • If primers are too short, they will cause non-specific annealing and end up amplifying nonspecific sequences. ...
411-4 OUTLINE I. Spontaneous mutation A. Single base pair
411-4 OUTLINE I. Spontaneous mutation A. Single base pair

... normally used in DNA (why thymine used instead) If Uracil were used in DNA, no way to distinguish cytosine deamination product as “abnormal” C deamination AUGCUG---------->A UGUUG ...
DNA Replication
DNA Replication

... • As the 2 DNA strands open at the origin, Replication Bubbles form • Human chromosomes have MANY bubbles but bacteria have one. Bubbles ...
The Molecular Basis of Inheritance
The Molecular Basis of Inheritance

... leading strand requires the formation of only a single primer as the replication fork continues to separate.  For synthesis of the lagging strand, each Okazaki fragment must be primed separately.  Another DNA polymerase, DNA polymerase I, replaces the RNA nucleotides of the primers with DNA versio ...
Chapter 16 The Molecular Basis of Inheritance
Chapter 16 The Molecular Basis of Inheritance

... leading strand requires the formation of only a single primer as the replication fork continues to separate.  For synthesis of the lagging strand, each Okazaki fragment must be primed separately.  Another DNA polymerase, DNA polymerase I, replaces the RNA nucleotides of the primers with DNA versio ...
Chapter 15: Protein Synthesis
Chapter 15: Protein Synthesis

... • Enzymes unwind the double helix and separate the two strands by breaking the hydrogen bonds between the nitrogenous bases only in the region where the gene to be transcribed is located • RNA polymerase synthesises messenger RNA (mRNA) using one of the strands of DNA as RNA polymerase a template ...
10 gene expression: transcription
10 gene expression: transcription

... The presence of two Laf proteins can be explained by alternative splicing of the laf+ mRNA. So let’s first try to figure out the coding region of the laf+ gene. The initiation codon will have to be an AUG. There are two codons early on: at positions 83–85 and 118–120. The latter is almost immediatel ...
MICROBIAL GENETICS
MICROBIAL GENETICS

... • Eukaryotes: more than one, linear chromosome ...
DNA
DNA

... •  RNA molecules usually exist as single polypeptide chains •  DNA molecules have two polynucleotides spiraling around an imaginary axis, forming a double helix •  In the DNA double helix, the two backbones run in opposite 5ʹ→ 3ʹ directions from each other, an arrangement referred to as antiparallel ...
PHYSgeneticsnotes
PHYSgeneticsnotes

6 Review of Molecular Biology
6 Review of Molecular Biology

Chapter 15 - jl041.k12.sd.us
Chapter 15 - jl041.k12.sd.us

... Prokaryotes have only one DNA molecule (circular and not protected by nuclear envelope) and this DNA molecule is not bound up with histones. Thus, gene regulation in prokaryotes is unique. One of the best known pathways of gene recognition is the lac Operon, a regulatory pathway by which bacteria ar ...
Gel Electrophoresis
Gel Electrophoresis

... Stages of DNA Profiling Stage ____:  Fragments are _______ by size using process called __________________.  DNA fragments ______ into wells and _______ current is applied along gel.  A _________ material is added which combines with the DNA fragments to produce a _________ ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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