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Glossary of Biotechnology Terms
Glossary of Biotechnology Terms

... corresponding clone can be amplified using PCR primed off the vector sequences connected to the ends of the cDNA. complement: The complement of a nucleic acid sequence replaces each base by its complementary base: adenine (A) by thymidine (T), cytosine (C) by guanine (G), and vice versa. In RNA, ade ...
Insertion of gene into plasmid
Insertion of gene into plasmid

... Put plasmid into bacterium by transformation ...
Manipulating DNA - Biology R: 4(A,C)
Manipulating DNA - Biology R: 4(A,C)

...  Gel electrophoresis is used to compare DNA of different organisms or identifying one particular gene ...
Jeopardy Review - Trimble County Schools
Jeopardy Review - Trimble County Schools

... Hodgepodge ...
Creating a Plasmid with a Human Gene
Creating a Plasmid with a Human Gene

... 1. Locate informational markers (DNA bands that are either present or absent in both parents). If one parent has the marker and the other does not, that marker is considered non-informational. 2. Examine the offspring’s marker. (If both parents have the marker then the offspring would possess the ma ...
Molecular Genetics II (cont.) Mutation
Molecular Genetics II (cont.) Mutation

... genes or translocations of genes from one chromosome to f h t another. Major rearrangements may or may not have phenotypic consequences. ...
PSI Genes- Homework
PSI Genes- Homework

... a. 3’ TGCATCTAATGC 5’ synthesized from right to left b. DNA polymerase c. It is semi-conservative because each new DNA molecule contains one parent strand and one daughter strand. a. Gene expression is the process of taking the code from DNA and transcribing it into mRNA, and then into the amino aci ...
Connectivity of Earth`s largest biomes: the deep Atlantic to the
Connectivity of Earth`s largest biomes: the deep Atlantic to the

... How do we measure genetic diversity and connectivity? • Comparisons will be made across populations within a species across time and space • Extract DNA from ~20-30 individuals per population • Recent advancements in DNA sequencing technology allows us to “scan” the entire genome • Method called: D ...
pGlo Power Point Presentation
pGlo Power Point Presentation

...  Originally evolved by bacteria  May express antibiotic resistance gene or be modified to express proteins of interest ...
Unit 5 Test Review 14-15
Unit 5 Test Review 14-15

... 19. __________ is the genetic material found in each cell in your body. One segment of this molecule is called a _______________. Every gene expresses itself as a _________________. Many proteins put together make up all of your _____________________. ...
Ross - Tree Improvement Program
Ross - Tree Improvement Program

... • Two copies are identical = “homozygous” • Two copies are different = “heterozygous” Homozygous parent ...
RNA Ribonucleic Acid - McKinney ISD Staff Sites
RNA Ribonucleic Acid - McKinney ISD Staff Sites

De novo sample preparation guidelines
De novo sample preparation guidelines

Protein Synthesis (Transcription and Translation)
Protein Synthesis (Transcription and Translation)

Gene Technology
Gene Technology

... itself 0 Ex: bacteria, yeasts ...
Figure S1 - G3: Genes | Genomes | Genetics
Figure S1 - G3: Genes | Genomes | Genetics

review final answers
review final answers

DNA
DNA

... Semi-conservative Replication • Two parent strands serve as templates for new complementary strands of DNA. • Replication occurs before a cell divides. ...
Nucleic acids
Nucleic acids

... or bacterium) contains plans for making all of its proteins. There are many thousands of kinds of proteins in each organism. Proteins are chains of building blocks called amino acids. The DNA specifies the order or sequence in which the amino acids must be hooked together for each protein. The compl ...
AP Biology
AP Biology

... 13. How are bacteriophages used for making genomic libraries and what are some of the advantages of this? ...
DNA: Transcription & Translation
DNA: Transcription & Translation

... DNA/ Genes/ Codons • DNA is made of approximately 80,000 genes • Genes are sections of DNA that code for a single protein ...
Apple Molecular Biology: Animation 1
Apple Molecular Biology: Animation 1

... Cloning can mean several things. Most people associate cloning with 'copying'. In molecular biology, cloning can be a process of recreating individuals from their own DNA but a more common use of cloning refers to the insertion of a short piece of DNA into a bacterial plasmid for replication purpose ...
DNA analysis - Madeira City Schools
DNA analysis - Madeira City Schools

... C. Things you need to know to understand what you are doing 1. Inserting the gene is not enough…you have to have something to turn it on 2. Turning on and off genes (gene expression) is carefully regulated to allow for adaptation to different conditions a. prevents wasteful production of unneeded p ...
Protein Synthesis PowerPoint - Scotts Valley High School
Protein Synthesis PowerPoint - Scotts Valley High School

... Genetic information is encoded in the sequence of the DNA double helix. To access this information, the DNA sequence must be copied, or "transcribed", by enzymes known as RNA polymerases. The resulting messenger RNA (mRNA) molecules carry the genetic information to the protein-synthesizing machinery ...
Fianl Exam Review
Fianl Exam Review

... a. The difference in DNA between animals, plants, bacteria… is the order of the nitrogen bases. b. The difference in DNA between animals, plants, bacteria… is the number of the nitrogen bases. c. Neither A nor B d. Both A and B 63. One codon on mRNA has how many nitrogen bases? a. 1 b. 3 c. random d ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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