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Eukaryotic Transcription
Eukaryotic Transcription

... Eukaryotic DNA Replication - similar machinery but: more complex structure (chromatin) so replication forks move slower; multiple replicons/origins that may have different activation times - α – primase, not processive - δ works on leading strand, and ε works on lagging strand; maybe not always - te ...
Overview of recombinant technology
Overview of recombinant technology

... A DNA sequence can be changed by copying errors introduced by DNA polymerase during replication and by environmental agents such as chemical mutagens or radiation If uncorrected, such changes may interfere with the ability of the cell to function DNA damage can be repaired by several mechanisms All ...
DNA
DNA

... • 12) The process in which one bacteria is changed by the transfer of genetic material from another bacteria is called ...
Unit 3: CHEMICAL PRINCIPLES:
Unit 3: CHEMICAL PRINCIPLES:

... DNA REPLICATION - One "parental" double-stranded DNA molecule is converted to two "daughter" molecules. The Parental DNA unwinds and separates from each other as the hydrogen bonds holding the nitrogen bases together breaks apart. Each side of the parental DNA joins with nucleotides having the appro ...
What is DNA?
What is DNA?

... and bond to the unpaired strands of DNA. 4. 2 identical DNA molecules are formed Product: - 2 DNA molecules that are exactly alike. **Replication is Semi-Conservative – meaning that the 2 old DNA halves are used as patterns on which to build to new halves. ...
Glucose - St. Bonaventure College and High School
Glucose - St. Bonaventure College and High School

... The allele for normal body pigmentation is dominant (D) and the allele for albino is recessive (d). Explain by means of a genetic diagram, the expected phenotypic ratio of the offspring when individual 7 is married to individual 10. ...
Practicing Protein Synthesis
Practicing Protein Synthesis

... defective DNA sequence that codes for the making of the insulin protein. Suppose a person has a mutation in their DNA and the first triplet for the insulin gene reads T A T. The normal gene reads T A G. What amino acid does the mutant DNA and the normal DNA code for and will the person with this mut ...
Answers to End-of-Chapter Questions – Brooker et al ARIS site
Answers to End-of-Chapter Questions – Brooker et al ARIS site

... Recombinant DNA: Any DNA molecule that has been manipulated so that it contains DNA from two or more sources. 2. Explain how using one restriction enzyme to cut both a plasmid and a gene of interest will allow the gene to be inserted into the plasmid. Answer: The restriction enzyme cuts the plasmid ...
Gene Expression
Gene Expression

... A mutation can be harmful, beneficial, or cause no change. The more similar a substitution, the less of a problem. But there is always a change in physiology. Ten percent of all breast cancers are congenital genetic mutations (the woman is born with it), the rest are from a spontaneous mutation of t ...
DNA to Protein
DNA to Protein

... Tumor Viruses – viral DNA into host DNA Viral DNA initiates tumor growth (oncogenes) In terms of viral survival why would a virus want to do this? – Viruses linked to: Lymphoma’s, cervical cancers, leukemia ...
RNA and Central Dogma
RNA and Central Dogma

Genetic Mutations & Genetic Engineering
Genetic Mutations & Genetic Engineering

... • Some neither help nor harm, some are beneficial for genetic variation, some cause disorders and cancer ...
Biotech PPT - Groch Biology
Biotech PPT - Groch Biology

... • Use forensic genetics to identify the remains of people murdered in El Salvador, Mexico and especially in Argentina. • Help the "Abuelas," the Grandmothers of the Plaza de Mayo in Buenos Aires. During Argentina's military dictatorship of the 1970s and early 1980s, thousands of activists disappeare ...
Section 1: The Structure of DNA Key Ideas • What is genetic material
Section 1: The Structure of DNA Key Ideas • What is genetic material

... Because DNA is made of two strands of complementary base pairs, if the strands are separated then each strand can serve as a pattern to make a new complementary strand. The process of making a copy of DNA is called DNA replication. In DNA replication, the DNA molecule unwinds, and the two sides spli ...
Section 12-1
Section 12-1

Chapter 16 - drtracey.net
Chapter 16 - drtracey.net

... creating DNA fragments. – Type I - simple cuts – Type II - dyad symmetry  allows physical mapping  allows recombinant molecules ...
Bio 102 Practice Problems The Double Helix
Bio 102 Practice Problems The Double Helix

... In double-stranded DNA, G pairs with C, so the amounts of these two bases should be equal: C=23% Now, G+C = 46%, so the remaining 54% must be A and T Since A pairs with T, these two should be equal; therefore, A=27% and T=27% 2. Why couldn't Hershey and Chase have used radioactive nitrogen in their ...
DNA - The Double Helix Worksheet
DNA - The Double Helix Worksheet

... Recall that the nucleus is a small spherical, dense body in a cell. It is often called the “control center” because it controls all the activities of the cell including cell reproduction, and heredity. Chromosomes are microscopic, threadlike strands composed of the chemical DNA (short for deoxyribon ...
Biotechnology Powerpoint
Biotechnology Powerpoint

... That the parental rights held by the Twiggs compelled that they be granted custody of 14year-old Kimberley Mays who was switched at birth with another newborn. ...
DNA VACCINES
DNA VACCINES

... ENDOGENOUS Antigenic Protein is presented by cell in which it is produced ...
Part 1 – History, DNA Structure, DNA Replication
Part 1 – History, DNA Structure, DNA Replication

... Click on “Finding the Structure” at the bottom of the page, then click on “putting it together” at the top of the new page. Click on the picture next to “base pairing interactive”. Go through the steps to determine how the nitrogen bases pair, and how the sugar phosphate backbone is formed. Draw you ...
made from DNA aptamers核酸适配体, which are short
made from DNA aptamers核酸适配体, which are short

... Researchers have found that O- linked β- N –acetyl-Dglucosamine ( O-GlcNAc) discourages memory formation when it’s attached to the transcription factor CREB and that memory improves when the sugar is removed or absent. The finding reveals a previously unknown sugar-based mechanism for regulating控制 g ...
Genetic Engineering Powerpoint
Genetic Engineering Powerpoint

... polymerase chain reaction (PCR) allows them to make many copies of it. 1. A piece of DNA is heated, which separates its two strands. ...
無投影片標題
無投影片標題

... Chemical structure of poly(D,L-lactide-co-glycolide) and its degradation products. ‘m’ and ‘n’ refer to the relative amounts of lactide and glycolide units respectively in a specific PLGA ...
Forensic DNA Analysis
Forensic DNA Analysis

... Single-cell sensitivity because each cell contains ~1000 mitochondria = very high contamination risk! Heteroplasmy - more than one mtDNA type manifesting in different tissues in the same individual Lower power of discrimination - maternal relatives all share the same mtDNA ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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