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History of DNA - Duplin County Schools
History of DNA - Duplin County Schools

... of bases in DNA to come up with his rules for base pairing. What are four sources of DNA that he used? http://fig.cox.miami.edu/~cmallery/150/gene/chargaff.htm Adenine (A) pairs with _____________ Guanine (G) pairs with _____________ ...
HL-SAN for DNA removal in protein purification - A4
HL-SAN for DNA removal in protein purification - A4

Chromosome Contact Matrices
Chromosome Contact Matrices

... Aggregation of TADs by their contact frequency ...
Biology Chapter 9
Biology Chapter 9

... Dolly the sheep was the first cloned mammal in 1997 Clones may not look exactly or act exactly like the original since gene expression is also affected by other factors like environment Being studied to help endangered species and creating human organs for transplants Controversial because the succe ...
PCR
PCR

Comparing DNA
Comparing DNA

PROTIEN SYNTHESIS
PROTIEN SYNTHESIS

... The form of RNA that mediates the transfer of genetic information from the cell nucleus to ribosomes in the cytoplasm, where it serves as a template for protein synthesis. It is synthesized from a DNA template during the process of transcription. transfer RNA One of a class of RNA molecules that tra ...
Slides - Department of Computer Science
Slides - Department of Computer Science

Introduction to DNA
Introduction to DNA

... This project is funded by a grant awarded under the President’s Community Based Job Training Grant as implemented by the U.S. Department of Labor’s Employment and Training Administration (CB-15-162-06-60). NCC is an equal opportunity employer and does not discriminate on the following basis: agains ...
The nucleotides
The nucleotides

... synthesis . There are three distinct size species of rRNA (23S, 16S, and 5S) in prokaryotic cells • In the eukaryotic cytosol, there are four rRNA size species (28S, 18S, 5.8S, and 5S). Transfer RNAs (tRNAs), the smallest of the three major species of RNA molecules (4S), There is at least one specif ...
Protein Synthesis - Ms. Bruce's Science Classes
Protein Synthesis - Ms. Bruce's Science Classes

... Occurs when chromosomes duplicate (make copies) The enzyme DNA polymerase adds new nucleotides Each old strand of nucleotides serves as a template for each new strand ...
BIOL 241 Nucleic Acids and Gene Expression I. Genes (Overview) A
BIOL 241 Nucleic Acids and Gene Expression I. Genes (Overview) A

... 2. cytosine and thymine------> pyrimidines - single ring D. Nucleotides bonded by phosphate groups 1. phosphates (5’) bind to sugars (3’) ---> dehydration synthesis 2. sugars and phosphates form “backbone” 3. bases project from backbone (forming side chains) 4. H-bonds can form between bases (on oth ...
BOOSTER PCR FOR LOW-COPY NUMBER SAMPLES
BOOSTER PCR FOR LOW-COPY NUMBER SAMPLES

Biotechnology II PPT
Biotechnology II PPT

... When the restriction enzyme cuts the DNA at the restriction site, it creates fragments of DNA called restriction fragments. Restriction fragments have “sticky ends” that can match up with the ends of other fragments. ...
MCDB 1041: Using DNA To manipulate DNA in the laboratory, one
MCDB 1041: Using DNA To manipulate DNA in the laboratory, one

... The  letters  E,  P,  B,  and  H  along  the  DNA  signify  specific  RE  sites:  nucleotide  sequences  where  restriction   enzymes  cut  the  DNA.    For  example  “E”  is  where  a  restriction  enzyme  called  Eco  RI  cuts. ...
Solutions  - MIT OpenCourseWare
Solutions - MIT OpenCourseWare

... A permanent alters the shape of hair by changing the location of the disulfide bridges within the hair. This is accomplished by first applying a reducing agent to reduce all of the existing disulfide bridges in the protein strands. Then the hair is curled into the desired shape, and an oxidizing age ...
DNA and the Genetic Code
DNA and the Genetic Code

Beginning to crack the code of `junk DNA`
Beginning to crack the code of `junk DNA`

Transcripton/Translation Worksheet
Transcripton/Translation Worksheet

... 7. Where is DNA found in the cell? nucleus Where is RNA found in the cell? Cytoplasm and nucleus 8. Name the three types of RNA and what they do. Messenger RNA (mRNA) carries the message that will be translated to form a protein. Ribosomal RNA (rRNA) forms part of ribosomes where proteins are made. ...
Chapter 7 I. DNA Structure DNA
Chapter 7 I. DNA Structure DNA

... Purine bonds to a Pyrimidine – Adenine +Thymine (Two hydrogen bonds) – Guanine +Cyotosine (Three hydrogen bonds) ...
Incomplete handout - the Conway Group
Incomplete handout - the Conway Group

DNA Amplification Reagents
DNA Amplification Reagents

Document
Document

... What is PCR? (basically a xerox machine) • DNA replication gone crazy in a tube! • Makes many copies of a specific target sequence from a small amount of template DNA • Affects gene mapping and cloning and DNA sequencing and detection • Applications in the detection of specific mutations, criminal ...
Review: Genetics
Review: Genetics

... allele into a virus that attacks a target cell and inserts the normal allele into the body. Cloning is the process of making a new identical copy of an organism from a single adult cell. Cloning can occur naturally as twins, or to genetically engineer plants and animals, endangered or extinct specie ...
recombinant DNA - Cloudfront.net
recombinant DNA - Cloudfront.net

... • genetic engineering is possible because of restriction enzymes (restriction endonucleases): • Very specific – recognize and then cut DNA molecules at specific base sequences called a restriction site (recognition sequence) – These are often a symmetrical series of four to eight bases on both stra ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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