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12–1 - cloudfront.net
12–1 - cloudfront.net

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... Your boss in the state crime lab gives you a sample of DNA obtained from the back side of a postage stamp. She asks you to do DNA fingerprinting on the sample in order to determine if it matches the DNA of a particular suspect. You recognize that you will have to amplify the DNA sample since there ...
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Replisome



The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.
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