DNA - anisam2
... Covalent bond between the phosphate group (attached to 5’ carbon) of one nucleotide and the 3’ carbon of the sugar of another nucleotide. This bond is very strong, and for this reason DNA is remarkably stable. DNA can be boiled and even autoclaved without degrading! 5’ and 3’ The ends of the DNA or ...
... Covalent bond between the phosphate group (attached to 5’ carbon) of one nucleotide and the 3’ carbon of the sugar of another nucleotide. This bond is very strong, and for this reason DNA is remarkably stable. DNA can be boiled and even autoclaved without degrading! 5’ and 3’ The ends of the DNA or ...
Fluorescent dye, SYBR Green, is incorporated into PCR reaction
... • Probably ~ 1 MB or more in humans • Need very many families to get closer than this in human, or very large populations ...
... • Probably ~ 1 MB or more in humans • Need very many families to get closer than this in human, or very large populations ...
Chapter 10 - Richsingiser.com
... • Length of 1.6 million nm (E. coli) • Compact and folded (E. coli cell is only 2000 nm long) • Eukaryotic DNA wrapped around histone proteins to form nucleosomes • Base pairs: A-T, G-C ...
... • Length of 1.6 million nm (E. coli) • Compact and folded (E. coli cell is only 2000 nm long) • Eukaryotic DNA wrapped around histone proteins to form nucleosomes • Base pairs: A-T, G-C ...
Protein Synthesis Activity
... 1. Colour your DNA strand so that the bases are different colours but all of the bases of the same type are the same colour. 2. Cut out the DNA and mRNA molecules. (mRNA is messenger RNA) TRANSCRIPTION: occurs in the nucleus 3. Create the nucleus on the left side of your desk by placing your DNA str ...
... 1. Colour your DNA strand so that the bases are different colours but all of the bases of the same type are the same colour. 2. Cut out the DNA and mRNA molecules. (mRNA is messenger RNA) TRANSCRIPTION: occurs in the nucleus 3. Create the nucleus on the left side of your desk by placing your DNA str ...
Unit: 2
... 21. Which type of reproduction creates the most opportunity for genetic diversity? Why is this important to life? 22. If a body cell has 52 chromosomes, how many chromosomes are in the haploid gamete? Unit: 7: 1. What is replication and when does it happen? Describe the 3 basic steps. 2. What is DNA ...
... 21. Which type of reproduction creates the most opportunity for genetic diversity? Why is this important to life? 22. If a body cell has 52 chromosomes, how many chromosomes are in the haploid gamete? Unit: 7: 1. What is replication and when does it happen? Describe the 3 basic steps. 2. What is DNA ...
Biologists have learned to manipulate DNA
... D. Prokaryotes waste little energy on unnecessary reactions due to many different operons II Regulation of Genes in Eukaryotes A. More elaborate and complicated than in prokaryotes B. Eukaryotic DNA includes promoter sequences before the point that transcription takes place C. Transcription factors- ...
... D. Prokaryotes waste little energy on unnecessary reactions due to many different operons II Regulation of Genes in Eukaryotes A. More elaborate and complicated than in prokaryotes B. Eukaryotic DNA includes promoter sequences before the point that transcription takes place C. Transcription factors- ...
DNA History and Structure
... is 3’ end – 5’ and 3’ refers to the carbon # on the pentose sugar that P or OH is attached to ...
... is 3’ end – 5’ and 3’ refers to the carbon # on the pentose sugar that P or OH is attached to ...
Structure of the Genome
... Sometimes nucleosomes are positioned in certain sites. This can have the effect of giving greater access or restricting access. The N-terminal of the core histones are not part of the tight DNA packing assembly and can be accessed even when the DNA is tightly wound around the octamer. Protease diges ...
... Sometimes nucleosomes are positioned in certain sites. This can have the effect of giving greater access or restricting access. The N-terminal of the core histones are not part of the tight DNA packing assembly and can be accessed even when the DNA is tightly wound around the octamer. Protease diges ...
Molecular Genetics
... The strand on the bottom runs in the opposite direction and is oriented 3′ to 5′. ...
... The strand on the bottom runs in the opposite direction and is oriented 3′ to 5′. ...
General Genetics Exam 1
... b) It must replicate accurately so that the information it contains is precisely inherited by the daughter cells c) It must be capable of undergoing occasional mutations, such that the information it carries is altered in a heritable way d) It must have highly repetitive DNA sequences. e) All are es ...
... b) It must replicate accurately so that the information it contains is precisely inherited by the daughter cells c) It must be capable of undergoing occasional mutations, such that the information it carries is altered in a heritable way d) It must have highly repetitive DNA sequences. e) All are es ...
Chapter 23 (Part 1)
... • The probability that a given piece of DNA will contain a specific restriction site is = n4 • n = the number of bases in the restriction site • So for a 6 base cutter (64), you would expect to find your site every ~1300 base pairs. So in a 10,000 bp fragment there is likely to by 7 or 8 restriction ...
... • The probability that a given piece of DNA will contain a specific restriction site is = n4 • n = the number of bases in the restriction site • So for a 6 base cutter (64), you would expect to find your site every ~1300 base pairs. So in a 10,000 bp fragment there is likely to by 7 or 8 restriction ...
Document
... • Some do not have a DNA typing facility owing to either a lack of resources or a lack of enough cases to warrant the investment. ...
... • Some do not have a DNA typing facility owing to either a lack of resources or a lack of enough cases to warrant the investment. ...
DNA Keychains - Kids in Need Foundation
... Step 5: Thread each end of the wire with the “bases” beads through the third and fourth beads from the bottom of each of the sugar and phosphate “uprights” and pull tight. You’ve made the first rung. Be sure that the “u-wire’s” ends are even. Step 6: Pull the ends of the bases wire into the center o ...
... Step 5: Thread each end of the wire with the “bases” beads through the third and fourth beads from the bottom of each of the sugar and phosphate “uprights” and pull tight. You’ve made the first rung. Be sure that the “u-wire’s” ends are even. Step 6: Pull the ends of the bases wire into the center o ...
Chapter 2
... The principal characteristics of the nomenclature aim for stability, meaningfulness, memorability and unambiguity. The nomenclature is documented using natural language and is mainly example-driven (see http://varnomen. hgvs.org). A formal definition of its syntax has been constructed in [Laros et a ...
... The principal characteristics of the nomenclature aim for stability, meaningfulness, memorability and unambiguity. The nomenclature is documented using natural language and is mainly example-driven (see http://varnomen. hgvs.org). A formal definition of its syntax has been constructed in [Laros et a ...
Designing Molecular Machines·
... protein to deduce what the gene looked like that gave rise to the protein, We want to do more than juSt find genes, which can comain as many as LOO,OOO letters. We now know that a change of one---or a fewieners out of the whole three bi llion is suffi cient to cause some diseases-not all, bur some. ...
... protein to deduce what the gene looked like that gave rise to the protein, We want to do more than juSt find genes, which can comain as many as LOO,OOO letters. We now know that a change of one---or a fewieners out of the whole three bi llion is suffi cient to cause some diseases-not all, bur some. ...
replicates
... Cells function according to the information contained in the master code of DNA (i.e., cell cycle, DNA to DNA, and DNA to RNA). ...
... Cells function according to the information contained in the master code of DNA (i.e., cell cycle, DNA to DNA, and DNA to RNA). ...
Lab 4 Restriction Analysis
... containing thousands of genes. The only way to break it into smaller segments was to physically shear it. But these fragments would be random, not reproducible, and were rapidly degraded by cellular nucleases if reintroduced into the cell. RE's, for the first time, allowed scientists to cut DNA into ...
... containing thousands of genes. The only way to break it into smaller segments was to physically shear it. But these fragments would be random, not reproducible, and were rapidly degraded by cellular nucleases if reintroduced into the cell. RE's, for the first time, allowed scientists to cut DNA into ...
Quiz-3
... was cloned using Pst-1 and restriction enzyme and the size of the recombinant plasmid is 5.0 kb. What will you do to confirm that you have the correct insert, and that it is the specific genes you wanted? Indicate the size of the DNA fragments on agarose gel. 23. What is RFLP and how is it useful fo ...
... was cloned using Pst-1 and restriction enzyme and the size of the recombinant plasmid is 5.0 kb. What will you do to confirm that you have the correct insert, and that it is the specific genes you wanted? Indicate the size of the DNA fragments on agarose gel. 23. What is RFLP and how is it useful fo ...
Replisome
The replisome is a complex molecular machine that carries out replication of DNA. The replisome first unwinds double stranded DNA into two single strands. For each of the resulting single strands, a new complementary sequence of DNA is synthesized. The net result is formation of two new double stranded DNA sequences that are exact copies of the original double stranded DNA sequence.In terms of structure, the replisome is composed of two replicative polymerase complexes, one of which synthesizes the leading strand, while the other synthesizes the lagging strand. The replisome is composed of a number of proteins including helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNA polymerase I, RNAse H, and ligase.